Experiment: R2A control for volatile experiments
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b22005 and SM_b20161 overlap by 4 nucleotides SM_b20161 and SM_b20162 are separated by 193 nucleotides SM_b20162 and SM_b20163 are separated by 44 nucleotides
SM_b22005: SM_b22005 - hypothetical protein, at 179,168 to 179,536
_b22005
SM_b20161: SM_b20161 - hypothetical protein, at 179,533 to 179,883
_b20161
SM_b20162: SM_b20162 - transcriptional regulator, at 180,077 to 180,766
_b20162
SM_b20163: SM_b20163 - hypothetical protein, at 180,811 to 181,980
_b20163
Position (kb) 180
181 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 179.088 kb on + strand at 179.089 kb on - strand at 179.089 kb on - strand at 179.089 kb on - strand at 179.089 kb on - strand at 179.089 kb on - strand at 179.089 kb on - strand at 179.497 kb on - strand, within SM_b22005 at 179.599 kb on + strand, within SM_b20161 at 179.601 kb on + strand, within SM_b20161 at 179.601 kb on + strand, within SM_b20161 at 179.602 kb on - strand, within SM_b20161 at 179.605 kb on - strand, within SM_b20161 at 179.670 kb on + strand, within SM_b20161 at 179.671 kb on - strand, within SM_b20161 at 179.671 kb on - strand, within SM_b20161 at 179.765 kb on + strand, within SM_b20161 at 179.766 kb on - strand, within SM_b20161 at 179.910 kb on + strand at 179.910 kb on + strand at 179.911 kb on - strand at 180.059 kb on + strand at 180.060 kb on - strand at 180.149 kb on + strand, within SM_b20162 at 180.150 kb on - strand, within SM_b20162 at 180.190 kb on + strand, within SM_b20162 at 180.190 kb on + strand, within SM_b20162 at 180.304 kb on - strand, within SM_b20162 at 180.442 kb on + strand, within SM_b20162 at 180.443 kb on - strand, within SM_b20162 at 180.466 kb on + strand, within SM_b20162 at 180.485 kb on + strand, within SM_b20162 at 180.486 kb on - strand, within SM_b20162 at 180.486 kb on - strand, within SM_b20162 at 180.486 kb on - strand, within SM_b20162 at 180.486 kb on - strand, within SM_b20162 at 180.486 kb on - strand, within SM_b20162 at 180.501 kb on - strand, within SM_b20162 at 180.673 kb on + strand, within SM_b20162 at 180.673 kb on + strand, within SM_b20162 at 180.673 kb on + strand, within SM_b20162 at 180.674 kb on - strand, within SM_b20162 at 180.741 kb on + strand at 180.742 kb on - strand at 181.261 kb on - strand, within SM_b20163 at 181.261 kb on - strand, within SM_b20163 at 181.535 kb on - strand, within SM_b20163 at 181.535 kb on - strand, within SM_b20163 at 181.535 kb on - strand, within SM_b20163 at 181.621 kb on + strand, within SM_b20163 at 181.621 kb on + strand, within SM_b20163 at 181.622 kb on - strand, within SM_b20163
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control for volatile experiments remove 179,088 + -0.4 179,089 - +0.2 179,089 - -0.7 179,089 - -1.2 179,089 - -0.9 179,089 - -1.2 179,089 - -0.9 179,497 - SM_b22005 0.89 -0.6 179,599 + SM_b20161 0.19 +1.1 179,601 + SM_b20161 0.19 +0.7 179,601 + SM_b20161 0.19 +0.7 179,602 - SM_b20161 0.20 -1.6 179,605 - SM_b20161 0.21 +0.8 179,670 + SM_b20161 0.39 +0.3 179,671 - SM_b20161 0.39 -0.2 179,671 - SM_b20161 0.39 -0.4 179,765 + SM_b20161 0.66 +0.5 179,766 - SM_b20161 0.66 -0.2 179,910 + -1.0 179,910 + +0.4 179,911 - -1.4 180,059 + +0.4 180,060 - -0.4 180,149 + SM_b20162 0.10 -0.7 180,150 - SM_b20162 0.11 +0.1 180,190 + SM_b20162 0.16 +0.2 180,190 + SM_b20162 0.16 -0.5 180,304 - SM_b20162 0.33 -0.7 180,442 + SM_b20162 0.53 +1.2 180,443 - SM_b20162 0.53 -0.5 180,466 + SM_b20162 0.56 -1.5 180,485 + SM_b20162 0.59 +0.0 180,486 - SM_b20162 0.59 -0.3 180,486 - SM_b20162 0.59 -0.3 180,486 - SM_b20162 0.59 -1.6 180,486 - SM_b20162 0.59 -2.1 180,486 - SM_b20162 0.59 -1.3 180,501 - SM_b20162 0.61 -1.9 180,673 + SM_b20162 0.86 +0.8 180,673 + SM_b20162 0.86 -0.1 180,673 + SM_b20162 0.86 -3.3 180,674 - SM_b20162 0.87 -0.9 180,741 + +0.7 180,742 - +0.0 181,261 - SM_b20163 0.38 +0.3 181,261 - SM_b20163 0.38 -0.1 181,535 - SM_b20163 0.62 -0.2 181,535 - SM_b20163 0.62 +0.2 181,535 - SM_b20163 0.62 -0.6 181,621 + SM_b20163 0.69 -0.3 181,621 + SM_b20163 0.69 +0.3 181,622 - SM_b20163 0.69 +0.1
Or see this region's nucleotide sequence