Experiment: Hydrogen/Acetate-Sulfite (90%atm/5mM-20mM) with 0.1% Yeast Extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3227 and cheY-3 are separated by 75 nucleotides cheY-3 and fliA are separated by 22 nucleotides fliA and DVU3230 overlap by 47 nucleotides DVU3230 and DVU3231 overlap by 1 nucleotides
DVU3227: DVU3227 - flagellar basal body-associated protein, putative (TIGR), at 3,395,658 to 3,396,341
DVU3227
DVU3228: cheY-3 - chemotaxis protein CheY (TIGR), at 3,396,417 to 3,396,797
cheY-3
DVU3229: fliA - RNA polymerase sigma factor for flagellar operon FliA (TIGR), at 3,396,820 to 3,397,617
fliA
DVU3230: DVU3230 - flagellar synthesis regulator FleN (TIGR), at 3,397,571 to 3,398,398
DVU3230
DVU3231: DVU3231 - flagellar biosynthesis protein FlhF, putative (TIGR), at 3,398,398 to 3,399,495
DVU3231
Position (kb)
3396
3397
3398 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3395.862 kb on - strand, within DVU3227 at 3395.937 kb on - strand, within DVU3227 at 3395.967 kb on - strand, within DVU3227 at 3396.004 kb on + strand, within DVU3227 at 3396.010 kb on + strand, within DVU3227 at 3396.012 kb on + strand, within DVU3227 at 3396.018 kb on - strand, within DVU3227 at 3396.077 kb on - strand, within DVU3227 at 3396.106 kb on + strand, within DVU3227 at 3396.134 kb on - strand, within DVU3227 at 3396.304 kb on + strand at 3396.310 kb on + strand at 3396.312 kb on - strand at 3396.443 kb on + strand at 3396.510 kb on - strand, within cheY-3 at 3396.566 kb on + strand, within cheY-3 at 3396.602 kb on + strand, within cheY-3 at 3396.646 kb on - strand, within cheY-3 at 3396.651 kb on - strand, within cheY-3 at 3396.673 kb on + strand, within cheY-3 at 3396.795 kb on + strand at 3396.797 kb on + strand at 3396.813 kb on - strand at 3396.817 kb on + strand at 3396.825 kb on - strand at 3396.825 kb on - strand at 3396.839 kb on + strand at 3396.876 kb on + strand at 3396.879 kb on + strand at 3397.086 kb on - strand, within fliA at 3397.262 kb on - strand, within fliA at 3397.401 kb on + strand, within fliA at 3397.417 kb on - strand, within fliA at 3397.417 kb on - strand, within fliA at 3397.436 kb on - strand, within fliA at 3397.453 kb on - strand, within fliA at 3397.459 kb on - strand, within fliA at 3397.472 kb on - strand, within fliA at 3397.513 kb on - strand, within fliA at 3397.610 kb on - strand at 3397.669 kb on + strand, within DVU3230 at 3398.159 kb on - strand, within DVU3230 at 3398.363 kb on + strand at 3398.477 kb on - strand at 3398.540 kb on + strand, within DVU3231 at 3398.563 kb on - strand, within DVU3231 at 3398.570 kb on - strand, within DVU3231
Per-strain Table
Position Strand Gene LocusTag Fraction Hydrogen/Acetate-Sulfite (90%atm/5mM-20mM) with 0.1% Yeast Extract remove 3,395,862 - DVU3227 0.30 -0.5 3,395,937 - DVU3227 0.41 -0.2 3,395,967 - DVU3227 0.45 -0.9 3,396,004 + DVU3227 0.51 -1.2 3,396,010 + DVU3227 0.51 -0.1 3,396,012 + DVU3227 0.52 +0.3 3,396,018 - DVU3227 0.53 -0.7 3,396,077 - DVU3227 0.61 -2.9 3,396,106 + DVU3227 0.65 -0.3 3,396,134 - DVU3227 0.70 -0.5 3,396,304 + +0.1 3,396,310 + -1.8 3,396,312 - -0.0 3,396,443 + -1.0 3,396,510 - cheY-3 DVU3228 0.24 -2.5 3,396,566 + cheY-3 DVU3228 0.39 -1.1 3,396,602 + cheY-3 DVU3228 0.49 -1.4 3,396,646 - cheY-3 DVU3228 0.60 +0.8 3,396,651 - cheY-3 DVU3228 0.61 -2.1 3,396,673 + cheY-3 DVU3228 0.67 -2.5 3,396,795 + -0.8 3,396,797 + -1.2 3,396,813 - +2.4 3,396,817 + -0.6 3,396,825 - +0.7 3,396,825 - +0.8 3,396,839 + -3.6 3,396,876 + -1.0 3,396,879 + -0.0 3,397,086 - fliA DVU3229 0.33 -1.0 3,397,262 - fliA DVU3229 0.55 +0.1 3,397,401 + fliA DVU3229 0.73 -0.5 3,397,417 - fliA DVU3229 0.75 -0.1 3,397,417 - fliA DVU3229 0.75 -0.6 3,397,436 - fliA DVU3229 0.77 +0.1 3,397,453 - fliA DVU3229 0.79 -0.7 3,397,459 - fliA DVU3229 0.80 -0.6 3,397,472 - fliA DVU3229 0.82 -0.3 3,397,513 - fliA DVU3229 0.87 -1.8 3,397,610 - -1.8 3,397,669 + DVU3230 0.12 +0.8 3,398,159 - DVU3230 0.71 -1.5 3,398,363 + -2.4 3,398,477 - -0.5 3,398,540 + DVU3231 0.13 +2.2 3,398,563 - DVU3231 0.15 +0.8 3,398,570 - DVU3231 0.16 -1.3
Or see this region's nucleotide sequence