Experiment: Hydrogen/Acetate-Thiosulfate (90%atm/5mM-20mM)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phrB and hisI are separated by 641 nucleotides hisI and hisG overlap by 4 nucleotides hisG and aroE are separated by 99 nucleotides
DVU0112: phrB - deoxyribodipyrimidine photolyase, putative (TIGR), at 145,051 to 146,475
phrB
DVU0113: hisI - phosphoribosyl-AMP cyclohydrolase (TIGR), at 147,117 to 147,497
hisI
DVU0114: hisG - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 147,494 to 148,375
hisG
DVU0115: aroE - shikimate 5-dehydrogenase (TIGR), at 148,475 to 149,380
aroE
Position (kb)
147
148 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 146.147 kb on - strand, within phrB at 146.156 kb on + strand, within phrB at 146.172 kb on - strand, within phrB at 146.308 kb on + strand, within phrB at 146.314 kb on - strand, within phrB at 146.316 kb on - strand, within phrB at 146.316 kb on - strand, within phrB at 146.338 kb on + strand at 146.346 kb on - strand at 146.362 kb on + strand at 146.362 kb on + strand at 146.364 kb on + strand at 146.372 kb on - strand at 146.387 kb on - strand at 146.506 kb on + strand at 146.542 kb on - strand at 146.547 kb on - strand at 146.563 kb on - strand at 146.575 kb on - strand at 146.612 kb on + strand at 146.627 kb on + strand at 146.627 kb on + strand at 146.652 kb on + strand at 146.660 kb on - strand at 146.704 kb on - strand at 146.704 kb on - strand at 146.708 kb on + strand at 146.739 kb on - strand at 146.796 kb on - strand at 146.881 kb on - strand at 146.882 kb on + strand at 146.883 kb on + strand at 146.912 kb on + strand at 146.912 kb on + strand at 146.912 kb on + strand at 146.920 kb on - strand at 146.939 kb on + strand at 146.939 kb on + strand at 146.947 kb on - strand at 146.948 kb on + strand at 146.956 kb on - strand at 146.971 kb on + strand at 146.971 kb on + strand at 147.098 kb on - strand at 147.215 kb on - strand, within hisI at 147.298 kb on - strand, within hisI at 147.304 kb on + strand, within hisI at 147.468 kb on - strand at 147.483 kb on - strand at 147.517 kb on - strand at 147.527 kb on + strand at 147.535 kb on - strand at 147.560 kb on - strand at 147.649 kb on - strand, within hisG at 147.680 kb on - strand, within hisG at 147.760 kb on + strand, within hisG at 147.821 kb on + strand, within hisG at 147.821 kb on + strand, within hisG at 147.828 kb on - strand, within hisG at 147.829 kb on - strand, within hisG at 147.829 kb on - strand, within hisG at 147.877 kb on - strand, within hisG at 147.894 kb on - strand, within hisG at 147.897 kb on - strand, within hisG at 147.946 kb on + strand, within hisG at 148.020 kb on - strand, within hisG at 148.071 kb on + strand, within hisG at 148.124 kb on - strand, within hisG at 148.172 kb on - strand, within hisG at 148.221 kb on + strand, within hisG at 148.280 kb on + strand, within hisG at 148.311 kb on + strand at 148.327 kb on + strand at 148.341 kb on - strand at 148.355 kb on + strand at 148.355 kb on + strand at 148.385 kb on + strand at 148.471 kb on + strand at 148.495 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hydrogen/Acetate-Thiosulfate (90%atm/5mM-20mM) remove 146,147 - phrB DVU0112 0.77 +2.6 146,156 + phrB DVU0112 0.78 +0.2 146,172 - phrB DVU0112 0.79 -0.5 146,308 + phrB DVU0112 0.88 -0.1 146,314 - phrB DVU0112 0.89 -0.3 146,316 - phrB DVU0112 0.89 -0.0 146,316 - phrB DVU0112 0.89 -0.5 146,338 + -0.9 146,346 - +0.3 146,362 + -0.5 146,362 + -0.2 146,364 + +0.1 146,372 - -0.5 146,387 - -0.2 146,506 + -0.3 146,542 - +1.9 146,547 - -0.7 146,563 - -1.2 146,575 - -0.5 146,612 + -0.3 146,627 + -0.3 146,627 + +0.1 146,652 + -0.3 146,660 - +1.6 146,704 - -0.3 146,704 - -0.1 146,708 + -1.1 146,739 - -0.9 146,796 - -0.5 146,881 - -0.2 146,882 + -0.1 146,883 + +1.6 146,912 + +0.5 146,912 + -0.1 146,912 + -0.0 146,920 - -0.6 146,939 + -0.1 146,939 + -0.2 146,947 - -2.4 146,948 + -0.2 146,956 - -0.2 146,971 + +0.8 146,971 + -0.9 147,098 - -0.0 147,215 - hisI DVU0113 0.26 -0.0 147,298 - hisI DVU0113 0.48 -0.7 147,304 + hisI DVU0113 0.49 -0.0 147,468 - +0.4 147,483 - -1.1 147,517 - -0.9 147,527 + -1.4 147,535 - +0.8 147,560 - +1.5 147,649 - hisG DVU0114 0.18 +0.5 147,680 - hisG DVU0114 0.21 +0.5 147,760 + hisG DVU0114 0.30 +0.3 147,821 + hisG DVU0114 0.37 -0.2 147,821 + hisG DVU0114 0.37 +0.1 147,828 - hisG DVU0114 0.38 -0.3 147,829 - hisG DVU0114 0.38 -1.5 147,829 - hisG DVU0114 0.38 -0.7 147,877 - hisG DVU0114 0.43 +0.3 147,894 - hisG DVU0114 0.45 -1.7 147,897 - hisG DVU0114 0.46 +1.0 147,946 + hisG DVU0114 0.51 +0.3 148,020 - hisG DVU0114 0.60 +2.6 148,071 + hisG DVU0114 0.65 -0.6 148,124 - hisG DVU0114 0.71 +0.6 148,172 - hisG DVU0114 0.77 -1.9 148,221 + hisG DVU0114 0.82 -0.6 148,280 + hisG DVU0114 0.89 -0.4 148,311 + -0.6 148,327 + +1.6 148,341 - -0.9 148,355 + -0.3 148,355 + -1.3 148,385 + -0.0 148,471 + -0.4 148,495 - -1.0
Or see this region's nucleotide sequence