Experiment: L-Lysine 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0481 and Psest_0482 are separated by 166 nucleotides Psest_0482 and Psest_0483 are separated by 63 nucleotides Psest_0483 and Psest_0484 overlap by 4 nucleotides Psest_0484 and Psest_0485 overlap by 4 nucleotides
Psest_0481: Psest_0481 - Predicted phosphohydrolases, at 530,851 to 531,666
_0481
Psest_0482: Psest_0482 - yqiA-like hydrolase, affects the cell envelope <small>(from data)</small>, at 531,833 to 532,438
_0482
Psest_0483: Psest_0483 - DNA topoisomerase IV, B subunit, proteobacterial, at 532,502 to 534,481
_0483
Psest_0484: Psest_0484 - hypothetical protein, at 534,478 to 535,461
_0484
Psest_0485: Psest_0485 - clan AA aspartic protease, TIGR02281 family, at 535,458 to 535,985
_0485
Position (kb) 532
533
534
535 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 531.662 kb on - strand at 531.738 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.802 kb on + strand at 531.802 kb on + strand at 531.803 kb on - strand at 531.803 kb on - strand at 531.820 kb on - strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 532.056 kb on - strand, within Psest_0482 at 532.058 kb on + strand, within Psest_0482 at 532.058 kb on + strand, within Psest_0482 at 532.058 kb on + strand, within Psest_0482 at 532.142 kb on + strand, within Psest_0482 at 532.166 kb on + strand, within Psest_0482 at 532.292 kb on + strand, within Psest_0482 at 532.313 kb on + strand, within Psest_0482 at 532.313 kb on + strand, within Psest_0482 at 532.313 kb on + strand, within Psest_0482 at 532.333 kb on + strand, within Psest_0482 at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 534.675 kb on + strand, within Psest_0484 at 534.759 kb on + strand, within Psest_0484 at 534.759 kb on + strand, within Psest_0484 at 534.841 kb on + strand, within Psest_0484 at 535.094 kb on + strand, within Psest_0484 at 535.095 kb on - strand, within Psest_0484 at 535.315 kb on + strand, within Psest_0484 at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand at 535.371 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 200 mM remove 531,662 - +0.9 531,738 + +1.5 531,746 + -2.5 531,746 + +0.5 531,746 + +0.8 531,746 + -0.8 531,746 + +0.3 531,746 + -0.3 531,747 - -2.9 531,747 - -0.7 531,747 - +0.3 531,747 - +0.5 531,747 - -2.5 531,747 - +0.6 531,802 + -0.2 531,802 + +1.0 531,803 - +0.9 531,803 - -0.5 531,820 - +0.4 531,860 + -1.7 531,860 + +2.7 531,860 + +1.2 531,860 + +1.8 531,860 + +2.3 531,860 + +1.4 531,860 + +1.1 531,860 + +2.3 531,860 + -0.6 531,861 - +1.5 531,861 - +1.4 531,861 - +2.0 531,861 - +0.9 531,861 - +1.6 531,861 - -0.0 531,861 - +1.7 531,862 + +0.2 531,862 + +3.1 531,862 + -1.0 531,862 + +1.2 531,862 + +1.9 531,862 + +0.1 531,862 + -1.6 531,862 + +0.2 531,862 + +2.0 531,862 + +1.4 531,862 + +1.8 531,862 + +2.2 531,862 - +0.2 531,863 - +1.3 531,863 - +1.4 531,863 - +0.3 531,863 - +0.5 531,863 - +1.2 531,863 - +2.0 531,863 - +0.9 531,863 - +1.3 531,863 - +0.2 531,863 - +1.4 531,863 - +1.0 531,863 - +0.4 531,863 - +2.6 531,864 + +1.6 531,864 + +1.6 531,864 + +0.5 531,864 + +1.0 531,864 + +2.3 531,864 + +1.4 531,864 + -0.1 531,864 + +0.0 531,864 + +1.7 531,864 + -0.2 531,864 + +0.2 531,864 + -0.2 531,865 - +1.1 531,865 - +1.0 531,865 - +1.3 531,865 - +0.5 531,865 - +1.6 531,865 - -0.8 531,865 - +1.8 531,865 - +0.8 531,865 - +0.9 531,865 - +1.6 531,865 - +0.9 531,865 - +1.2 531,865 - +0.4 532,056 - Psest_0482 0.37 -1.1 532,058 + Psest_0482 0.37 +0.6 532,058 + Psest_0482 0.37 +2.0 532,058 + Psest_0482 0.37 +2.0 532,142 + Psest_0482 0.51 +1.4 532,166 + Psest_0482 0.55 +3.2 532,292 + Psest_0482 0.76 +2.5 532,313 + Psest_0482 0.79 +1.8 532,313 + Psest_0482 0.79 -0.2 532,313 + Psest_0482 0.79 +0.5 532,333 + Psest_0482 0.83 +2.3 532,576 + -0.2 532,576 + -1.0 532,576 + -0.6 532,576 + +0.6 532,576 + +0.8 532,576 + -0.1 532,576 + +0.1 532,576 + -0.8 532,576 + +0.6 532,576 + +0.8 534,675 + Psest_0484 0.20 +1.3 534,759 + Psest_0484 0.29 +0.3 534,759 + Psest_0484 0.29 +1.5 534,841 + Psest_0484 0.37 -0.5 535,094 + Psest_0484 0.63 -3.4 535,095 - Psest_0484 0.63 +0.0 535,315 + Psest_0484 0.85 +1.5 535,371 + +1.0 535,371 + +1.2 535,371 + -1.1 535,371 + +1.9 535,371 + +0.7
Or see this region's nucleotide sequence