Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0996

Experiment: Choline Lysine 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0994 and Psest_0995 are separated by 83 nucleotidesPsest_0995 and Psest_0996 overlap by 4 nucleotidesPsest_0996 and Psest_0997 are separated by 123 nucleotidesPsest_0997 and Psest_0998 are separated by 1 nucleotides Psest_0994: Psest_0994 - L-aspartate-alpha-decarboxylase, at 1,077,686 to 1,078,066 _0994 Psest_0995: Psest_0995 - pantoate--beta-alanine ligase, at 1,078,150 to 1,079,010 _0995 Psest_0996: Psest_0996 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,079,007 to 1,079,807 _0996 Psest_0997: Psest_0997 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase, at 1,079,931 to 1,080,458 _0997 Psest_0998: Psest_0998 - poly(A) polymerase, at 1,080,460 to 1,081,854 _0998 Position (kb) 1079 1080Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1079.895 kb on - strandat 1079.895 kb on - strandat 1079.895 kb on - strandat 1079.895 kb on - strandat 1079.895 kb on - strandat 1079.915 kb on - strandat 1079.915 kb on - strandat 1079.915 kb on - strandat 1079.915 kb on - strandat 1079.915 kb on - strandat 1079.915 kb on - strandat 1079.915 kb on - strandat 1079.943 kb on + strandat 1079.944 kb on - strandat 1079.944 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 20 mM
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1,079,895 - +2.3
1,079,895 - +2.1
1,079,895 - +2.4
1,079,895 - +2.1
1,079,895 - +2.5
1,079,915 - +2.4
1,079,915 - +1.9
1,079,915 - +1.3
1,079,915 - +3.2
1,079,915 - +2.9
1,079,915 - +2.5
1,079,915 - +1.8
1,079,943 + -1.5
1,079,944 - -2.0
1,079,944 - -0.2

Or see this region's nucleotide sequence