Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0515 and Psest_0516 are separated by 48 nucleotides Psest_0516 and Psest_0517 are separated by 66 nucleotides Psest_0517 and Psest_0518 overlap by 1 nucleotides
Psest_0515: Psest_0515 - rare lipoprotein A, at 568,809 to 569,801
_0515
Psest_0516: Psest_0516 - D-alanyl-D-alanine carboxypeptidase, at 569,850 to 571,007
_0516
Psest_0517: Psest_0517 - Uncharacterized conserved protein, at 571,074 to 571,355
_0517
Psest_0518: Psest_0518 - lipoate-protein ligase B, at 571,355 to 572,011
_0518
Psest_0519: Psest_0519 - lipoate synthase, at 572,004 to 572,981
_0519
Position (kb) 569
570
571
572 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 569.025 kb on + strand, within Psest_0515 at 569.026 kb on - strand, within Psest_0515 at 569.026 kb on - strand, within Psest_0515 at 569.026 kb on - strand, within Psest_0515 at 569.040 kb on + strand, within Psest_0515 at 569.061 kb on + strand, within Psest_0515 at 569.061 kb on + strand, within Psest_0515 at 569.061 kb on + strand, within Psest_0515 at 569.061 kb on + strand, within Psest_0515 at 569.061 kb on + strand, within Psest_0515 at 569.160 kb on + strand, within Psest_0515 at 569.211 kb on + strand, within Psest_0515 at 569.211 kb on + strand, within Psest_0515 at 569.302 kb on + strand, within Psest_0515 at 569.674 kb on + strand, within Psest_0515 at 569.675 kb on - strand, within Psest_0515 at 569.675 kb on - strand, within Psest_0515 at 569.818 kb on - strand at 569.818 kb on - strand at 569.818 kb on - strand at 569.826 kb on + strand at 569.826 kb on + strand at 569.827 kb on - strand at 569.881 kb on + strand at 569.881 kb on + strand at 569.882 kb on - strand at 569.882 kb on - strand at 569.882 kb on - strand at 569.996 kb on - strand, within Psest_0516 at 570.060 kb on + strand, within Psest_0516 at 570.061 kb on - strand, within Psest_0516 at 570.061 kb on - strand, within Psest_0516 at 570.061 kb on - strand, within Psest_0516 at 570.061 kb on - strand, within Psest_0516 at 570.061 kb on - strand, within Psest_0516 at 570.061 kb on - strand, within Psest_0516 at 570.061 kb on - strand, within Psest_0516 at 570.061 kb on - strand, within Psest_0516 at 570.152 kb on + strand, within Psest_0516 at 570.153 kb on - strand, within Psest_0516 at 570.153 kb on - strand, within Psest_0516 at 570.284 kb on + strand, within Psest_0516 at 570.284 kb on + strand, within Psest_0516 at 570.284 kb on + strand, within Psest_0516 at 570.285 kb on - strand, within Psest_0516 at 570.285 kb on - strand, within Psest_0516 at 570.285 kb on - strand, within Psest_0516 at 570.285 kb on - strand, within Psest_0516 at 570.408 kb on + strand, within Psest_0516 at 570.408 kb on + strand, within Psest_0516 at 570.408 kb on + strand, within Psest_0516 at 570.408 kb on + strand, within Psest_0516 at 570.408 kb on + strand, within Psest_0516 at 570.408 kb on + strand, within Psest_0516 at 570.408 kb on + strand, within Psest_0516 at 570.408 kb on + strand, within Psest_0516 at 570.408 kb on + strand, within Psest_0516 at 570.408 kb on + strand, within Psest_0516 at 570.408 kb on + strand, within Psest_0516 at 570.409 kb on - strand, within Psest_0516 at 570.409 kb on - strand, within Psest_0516 at 570.409 kb on - strand, within Psest_0516 at 570.409 kb on - strand, within Psest_0516 at 570.435 kb on + strand, within Psest_0516 at 570.435 kb on + strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.436 kb on - strand, within Psest_0516 at 570.657 kb on + strand, within Psest_0516 at 570.658 kb on - strand, within Psest_0516 at 570.690 kb on + strand, within Psest_0516 at 570.691 kb on - strand, within Psest_0516 at 570.702 kb on - strand, within Psest_0516 at 570.971 kb on + strand at 570.990 kb on + strand at 572.003 kb on + strand at 572.003 kb on + strand at 572.003 kb on + strand at 572.003 kb on + strand at 572.003 kb on + strand at 572.003 kb on + strand at 572.003 kb on + strand at 572.003 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 569,025 + Psest_0515 0.22 -2.5 569,026 - Psest_0515 0.22 +0.7 569,026 - Psest_0515 0.22 +0.7 569,026 - Psest_0515 0.22 +0.7 569,040 + Psest_0515 0.23 -0.1 569,061 + Psest_0515 0.25 -0.1 569,061 + Psest_0515 0.25 -1.8 569,061 + Psest_0515 0.25 -3.3 569,061 + Psest_0515 0.25 -1.7 569,061 + Psest_0515 0.25 +0.7 569,160 + Psest_0515 0.35 -0.7 569,211 + Psest_0515 0.40 -0.1 569,211 + Psest_0515 0.40 -0.7 569,302 + Psest_0515 0.50 -1.1 569,674 + Psest_0515 0.87 -0.9 569,675 - Psest_0515 0.87 -2.1 569,675 - Psest_0515 0.87 -2.2 569,818 - -2.4 569,818 - -1.6 569,818 - -4.1 569,826 + +0.1 569,826 + -2.1 569,827 - -2.8 569,881 + -1.5 569,881 + -2.3 569,882 - -2.8 569,882 - +0.7 569,882 - -3.4 569,996 - Psest_0516 0.13 -1.3 570,060 + Psest_0516 0.18 -2.5 570,061 - Psest_0516 0.18 -3.1 570,061 - Psest_0516 0.18 -1.1 570,061 - Psest_0516 0.18 -0.2 570,061 - Psest_0516 0.18 -3.4 570,061 - Psest_0516 0.18 -1.6 570,061 - Psest_0516 0.18 -3.0 570,061 - Psest_0516 0.18 -1.1 570,061 - Psest_0516 0.18 -2.1 570,152 + Psest_0516 0.26 -0.8 570,153 - Psest_0516 0.26 -1.2 570,153 - Psest_0516 0.26 -0.8 570,284 + Psest_0516 0.37 -0.9 570,284 + Psest_0516 0.37 -2.6 570,284 + Psest_0516 0.37 -2.3 570,285 - Psest_0516 0.38 -1.2 570,285 - Psest_0516 0.38 -1.9 570,285 - Psest_0516 0.38 -2.8 570,285 - Psest_0516 0.38 -0.7 570,408 + Psest_0516 0.48 -1.8 570,408 + Psest_0516 0.48 -1.1 570,408 + Psest_0516 0.48 -1.3 570,408 + Psest_0516 0.48 -1.1 570,408 + Psest_0516 0.48 -1.8 570,408 + Psest_0516 0.48 -2.2 570,408 + Psest_0516 0.48 -1.5 570,408 + Psest_0516 0.48 -0.9 570,408 + Psest_0516 0.48 -2.8 570,408 + Psest_0516 0.48 -0.9 570,408 + Psest_0516 0.48 -2.1 570,409 - Psest_0516 0.48 -1.9 570,409 - Psest_0516 0.48 -0.3 570,409 - Psest_0516 0.48 -2.3 570,409 - Psest_0516 0.48 -1.3 570,435 + Psest_0516 0.51 +0.2 570,435 + Psest_0516 0.51 -2.2 570,436 - Psest_0516 0.51 -1.6 570,436 - Psest_0516 0.51 -2.6 570,436 - Psest_0516 0.51 -2.6 570,436 - Psest_0516 0.51 -1.6 570,436 - Psest_0516 0.51 +0.4 570,436 - Psest_0516 0.51 -2.0 570,436 - Psest_0516 0.51 -2.9 570,436 - Psest_0516 0.51 -2.9 570,436 - Psest_0516 0.51 -2.0 570,436 - Psest_0516 0.51 -0.8 570,436 - Psest_0516 0.51 -1.8 570,436 - Psest_0516 0.51 +1.2 570,436 - Psest_0516 0.51 -0.3 570,436 - Psest_0516 0.51 -3.5 570,436 - Psest_0516 0.51 -2.0 570,657 + Psest_0516 0.70 -2.2 570,658 - Psest_0516 0.70 -2.0 570,690 + Psest_0516 0.73 -1.6 570,691 - Psest_0516 0.73 -2.5 570,702 - Psest_0516 0.74 -1.6 570,971 + -0.8 570,990 + +0.2 572,003 + +1.7 572,003 + +2.6 572,003 + +2.7 572,003 + +1.4 572,003 + +0.5 572,003 + +2.1 572,003 + +2.8 572,003 + -0.9
Or see this region's nucleotide sequence