Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_4132

Experiment: Choline Lysine 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_4131 and Psest_4132 are separated by 31 nucleotidesPsest_4132 and Psest_4133 are separated by 315 nucleotides Psest_4131: Psest_4131 - alpha-ketoglutarate sensor protein (mifS) (from data), at 4,310,657 to 4,312,420 _4131 Psest_4132: Psest_4132 - alpha-ketoglutarate DNA-binding response regulator (mifR) (from data), at 4,312,452 to 4,313,795 _4132 Psest_4133: Psest_4133 - RNA polymerase sigma factor, sigma-70 family, at 4,314,111 to 4,315,352 _4133 Position (kb) 4312 4313 4314Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4311.513 kb on + strand, within Psest_4131at 4311.513 kb on + strand, within Psest_4131at 4311.514 kb on - strand, within Psest_4131at 4311.514 kb on - strand, within Psest_4131at 4311.514 kb on - strand, within Psest_4131at 4311.612 kb on - strand, within Psest_4131at 4311.981 kb on + strand, within Psest_4131at 4312.384 kb on + strandat 4312.465 kb on + strandat 4312.465 kb on + strandat 4312.465 kb on + strandat 4312.465 kb on + strandat 4312.466 kb on - strandat 4312.466 kb on - strandat 4312.743 kb on + strand, within Psest_4132at 4312.907 kb on + strand, within Psest_4132at 4312.908 kb on - strand, within Psest_4132at 4313.255 kb on - strand, within Psest_4132at 4313.384 kb on + strand, within Psest_4132at 4313.384 kb on + strand, within Psest_4132at 4313.385 kb on - strand, within Psest_4132at 4313.385 kb on - strand, within Psest_4132at 4313.766 kb on + strandat 4313.766 kb on + strandat 4313.766 kb on + strandat 4313.766 kb on + strandat 4313.766 kb on + strandat 4313.767 kb on - strandat 4313.767 kb on - strandat 4313.767 kb on - strandat 4313.767 kb on - strandat 4313.767 kb on - strandat 4313.789 kb on + strandat 4314.021 kb on + strandat 4314.022 kb on - strandat 4314.053 kb on - strandat 4314.053 kb on - strandat 4314.053 kb on - strandat 4314.053 kb on - strandat 4314.213 kb on - strandat 4314.213 kb on - strandat 4314.213 kb on - strandat 4314.213 kb on - strandat 4314.262 kb on + strand, within Psest_4133at 4314.397 kb on + strand, within Psest_4133at 4314.398 kb on - strand, within Psest_4133at 4314.577 kb on - strand, within Psest_4133at 4314.757 kb on + strand, within Psest_4133at 4314.758 kb on - strand, within Psest_4133at 4314.758 kb on - strand, within Psest_4133at 4314.758 kb on - strand, within Psest_4133at 4314.758 kb on - strand, within Psest_4133at 4314.778 kb on + strand, within Psest_4133at 4314.778 kb on + strand, within Psest_4133at 4314.778 kb on + strand, within Psest_4133at 4314.779 kb on - strand, within Psest_4133at 4314.779 kb on - strand, within Psest_4133at 4314.779 kb on - strand, within Psest_4133at 4314.779 kb on - strand, within Psest_4133

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline Lysine 20 mM
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4,311,513 + Psest_4131 0.49 -2.4
4,311,513 + Psest_4131 0.49 +0.1
4,311,514 - Psest_4131 0.49 +0.5
4,311,514 - Psest_4131 0.49 +0.4
4,311,514 - Psest_4131 0.49 -0.5
4,311,612 - Psest_4131 0.54 -0.3
4,311,981 + Psest_4131 0.75 +1.9
4,312,384 + +0.3
4,312,465 + +1.0
4,312,465 + -1.8
4,312,465 + -0.0
4,312,465 + -2.0
4,312,466 - -0.6
4,312,466 - -0.3
4,312,743 + Psest_4132 0.22 +0.6
4,312,907 + Psest_4132 0.34 -1.6
4,312,908 - Psest_4132 0.34 -0.7
4,313,255 - Psest_4132 0.60 -3.6
4,313,384 + Psest_4132 0.69 -0.1
4,313,384 + Psest_4132 0.69 -2.0
4,313,385 - Psest_4132 0.69 -1.0
4,313,385 - Psest_4132 0.69 -0.0
4,313,766 + -0.3
4,313,766 + +2.4
4,313,766 + -1.9
4,313,766 + +0.1
4,313,766 + -1.3
4,313,767 - -0.9
4,313,767 - -2.2
4,313,767 - -0.4
4,313,767 - -0.9
4,313,767 - +1.4
4,313,789 + -1.3
4,314,021 + +0.4
4,314,022 - +0.7
4,314,053 - +0.3
4,314,053 - +0.1
4,314,053 - -1.2
4,314,053 - +0.5
4,314,213 - -1.0
4,314,213 - +1.7
4,314,213 - +1.6
4,314,213 - +0.8
4,314,262 + Psest_4133 0.12 +1.2
4,314,397 + Psest_4133 0.23 -1.2
4,314,398 - Psest_4133 0.23 -0.0
4,314,577 - Psest_4133 0.38 +0.2
4,314,757 + Psest_4133 0.52 -2.2
4,314,758 - Psest_4133 0.52 +0.6
4,314,758 - Psest_4133 0.52 -1.0
4,314,758 - Psest_4133 0.52 -1.2
4,314,758 - Psest_4133 0.52 -1.1
4,314,778 + Psest_4133 0.54 -0.5
4,314,778 + Psest_4133 0.54 +1.6
4,314,778 + Psest_4133 0.54 -1.2
4,314,779 - Psest_4133 0.54 +1.2
4,314,779 - Psest_4133 0.54 -0.7
4,314,779 - Psest_4133 0.54 -0.7
4,314,779 - Psest_4133 0.54 +1.0

Or see this region's nucleotide sequence