Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1964 and Psest_1965 overlap by 4 nucleotides Psest_1965 and Psest_1966 are separated by 3 nucleotides Psest_1966 and Psest_1967 are separated by 168 nucleotides Psest_1967 and Psest_1968 are separated by 230 nucleotides
Psest_1964: Psest_1964 - hypothetical protein, at 2,078,544 to 2,078,945
_1964
Psest_1965: Psest_1965 - ABC-type bacteriocin/lantibiotic exporters, contain an N-terminal double-glycine peptidase domain, at 2,078,942 to 2,079,895
_1965
Psest_1966: Psest_1966 - Glycine/D-amino acid oxidases (deaminating), at 2,079,899 to 2,081,182
_1966
Psest_1967: Psest_1967 - tRNA_Val_GAC, at 2,081,351 to 2,081,427
_1967
Psest_1968: Psest_1968 - threonyl-tRNA synthetase, at 2,081,658 to 2,083,580
_1968
Position (kb)
2079
2080
2081
2082 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2078.961 kb on + strand at 2078.961 kb on + strand at 2078.961 kb on + strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2078.962 kb on - strand at 2079.007 kb on + strand at 2079.065 kb on + strand, within Psest_1965 at 2079.066 kb on - strand, within Psest_1965 at 2079.080 kb on + strand, within Psest_1965 at 2079.164 kb on + strand, within Psest_1965 at 2079.164 kb on + strand, within Psest_1965 at 2079.164 kb on + strand, within Psest_1965 at 2079.164 kb on + strand, within Psest_1965 at 2079.164 kb on + strand, within Psest_1965 at 2079.165 kb on - strand, within Psest_1965 at 2079.165 kb on - strand, within Psest_1965 at 2079.195 kb on + strand, within Psest_1965 at 2079.195 kb on + strand, within Psest_1965 at 2079.237 kb on - strand, within Psest_1965 at 2079.400 kb on + strand, within Psest_1965 at 2079.742 kb on - strand, within Psest_1965 at 2079.927 kb on + strand at 2079.927 kb on + strand at 2079.928 kb on - strand at 2079.928 kb on - strand at 2079.928 kb on - strand at 2079.928 kb on - strand at 2079.928 kb on - strand at 2079.930 kb on + strand at 2079.931 kb on - strand at 2079.931 kb on - strand at 2079.931 kb on - strand at 2079.988 kb on - strand at 2080.069 kb on + strand, within Psest_1966 at 2080.070 kb on - strand, within Psest_1966 at 2080.071 kb on + strand, within Psest_1966 at 2080.071 kb on + strand, within Psest_1966 at 2080.071 kb on + strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.072 kb on - strand, within Psest_1966 at 2080.105 kb on - strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.107 kb on + strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.108 kb on - strand, within Psest_1966 at 2080.112 kb on - strand, within Psest_1966 at 2080.179 kb on + strand, within Psest_1966 at 2080.179 kb on + strand, within Psest_1966 at 2080.180 kb on - strand, within Psest_1966 at 2080.216 kb on + strand, within Psest_1966 at 2080.269 kb on + strand, within Psest_1966 at 2080.270 kb on - strand, within Psest_1966 at 2080.287 kb on + strand, within Psest_1966 at 2080.287 kb on + strand, within Psest_1966 at 2080.287 kb on + strand, within Psest_1966 at 2080.288 kb on - strand, within Psest_1966 at 2080.320 kb on + strand, within Psest_1966 at 2080.320 kb on + strand, within Psest_1966 at 2080.320 kb on + strand, within Psest_1966 at 2080.332 kb on + strand, within Psest_1966 at 2080.468 kb on - strand, within Psest_1966 at 2080.468 kb on - strand, within Psest_1966 at 2080.519 kb on + strand, within Psest_1966 at 2080.520 kb on - strand, within Psest_1966 at 2080.697 kb on + strand, within Psest_1966 at 2080.705 kb on + strand, within Psest_1966 at 2080.706 kb on - strand, within Psest_1966 at 2080.802 kb on + strand, within Psest_1966 at 2080.802 kb on + strand, within Psest_1966 at 2080.803 kb on - strand, within Psest_1966 at 2080.831 kb on + strand, within Psest_1966 at 2080.836 kb on + strand, within Psest_1966 at 2080.837 kb on - strand, within Psest_1966 at 2080.945 kb on + strand, within Psest_1966 at 2080.947 kb on + strand, within Psest_1966 at 2080.947 kb on + strand, within Psest_1966 at 2080.947 kb on + strand, within Psest_1966 at 2080.948 kb on - strand, within Psest_1966 at 2080.948 kb on - strand, within Psest_1966 at 2080.948 kb on - strand, within Psest_1966 at 2080.948 kb on - strand, within Psest_1966 at 2080.951 kb on + strand, within Psest_1966 at 2080.951 kb on + strand, within Psest_1966 at 2080.951 kb on + strand, within Psest_1966 at 2080.951 kb on + strand, within Psest_1966 at 2080.952 kb on - strand, within Psest_1966 at 2081.067 kb on + strand at 2081.151 kb on + strand at 2081.151 kb on + strand at 2081.154 kb on + strand at 2081.154 kb on + strand at 2081.154 kb on + strand at 2081.154 kb on + strand at 2081.155 kb on - strand at 2081.155 kb on - strand at 2081.155 kb on - strand at 2081.155 kb on - strand at 2081.155 kb on - strand at 2081.182 kb on + strand at 2081.183 kb on - strand at 2081.189 kb on + strand at 2081.261 kb on - strand at 2081.261 kb on - strand at 2081.261 kb on - strand at 2081.264 kb on + strand at 2081.264 kb on + strand at 2081.283 kb on + strand at 2081.295 kb on - strand at 2081.629 kb on + strand at 2081.642 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 2,078,961 + +0.8 2,078,961 + -0.4 2,078,961 + -1.4 2,078,962 - +0.8 2,078,962 - -0.0 2,078,962 - +1.0 2,078,962 - +1.7 2,078,962 - -0.3 2,078,962 - -0.6 2,078,962 - -3.2 2,078,962 - +0.3 2,078,962 - +0.8 2,079,007 + -0.2 2,079,065 + Psest_1965 0.13 +0.6 2,079,066 - Psest_1965 0.13 -1.0 2,079,080 + Psest_1965 0.14 -1.0 2,079,164 + Psest_1965 0.23 +0.5 2,079,164 + Psest_1965 0.23 -0.0 2,079,164 + Psest_1965 0.23 +1.0 2,079,164 + Psest_1965 0.23 +1.0 2,079,164 + Psest_1965 0.23 +0.8 2,079,165 - Psest_1965 0.23 +0.2 2,079,165 - Psest_1965 0.23 -0.7 2,079,195 + Psest_1965 0.27 -0.9 2,079,195 + Psest_1965 0.27 -1.6 2,079,237 - Psest_1965 0.31 -0.1 2,079,400 + Psest_1965 0.48 -1.0 2,079,742 - Psest_1965 0.84 +1.8 2,079,927 + -0.7 2,079,927 + -0.5 2,079,928 - +0.1 2,079,928 - -0.2 2,079,928 - -0.5 2,079,928 - -0.4 2,079,928 - -0.0 2,079,930 + -0.0 2,079,931 - +1.1 2,079,931 - -2.1 2,079,931 - -0.2 2,079,988 - +1.2 2,080,069 + Psest_1966 0.13 +0.3 2,080,070 - Psest_1966 0.13 +0.1 2,080,071 + Psest_1966 0.13 -0.0 2,080,071 + Psest_1966 0.13 +0.3 2,080,071 + Psest_1966 0.13 +1.6 2,080,072 - Psest_1966 0.13 -0.8 2,080,072 - Psest_1966 0.13 +0.3 2,080,072 - Psest_1966 0.13 -2.2 2,080,072 - Psest_1966 0.13 +0.5 2,080,072 - Psest_1966 0.13 +0.2 2,080,072 - Psest_1966 0.13 +0.8 2,080,105 - Psest_1966 0.16 -1.8 2,080,107 + Psest_1966 0.16 -1.5 2,080,107 + Psest_1966 0.16 -0.8 2,080,107 + Psest_1966 0.16 -0.2 2,080,107 + Psest_1966 0.16 -1.6 2,080,107 + Psest_1966 0.16 +0.4 2,080,107 + Psest_1966 0.16 -1.5 2,080,107 + Psest_1966 0.16 +0.4 2,080,107 + Psest_1966 0.16 -2.6 2,080,107 + Psest_1966 0.16 -0.2 2,080,108 - Psest_1966 0.16 -1.5 2,080,108 - Psest_1966 0.16 -0.8 2,080,108 - Psest_1966 0.16 -2.5 2,080,108 - Psest_1966 0.16 +0.2 2,080,108 - Psest_1966 0.16 -0.6 2,080,108 - Psest_1966 0.16 -0.5 2,080,108 - Psest_1966 0.16 -1.5 2,080,108 - Psest_1966 0.16 -0.4 2,080,108 - Psest_1966 0.16 +0.2 2,080,108 - Psest_1966 0.16 -0.3 2,080,108 - Psest_1966 0.16 -0.7 2,080,112 - Psest_1966 0.17 -0.2 2,080,179 + Psest_1966 0.22 +0.5 2,080,179 + Psest_1966 0.22 -1.2 2,080,180 - Psest_1966 0.22 -2.9 2,080,216 + Psest_1966 0.25 -0.2 2,080,269 + Psest_1966 0.29 -0.9 2,080,270 - Psest_1966 0.29 +0.5 2,080,287 + Psest_1966 0.30 -0.8 2,080,287 + Psest_1966 0.30 +0.2 2,080,287 + Psest_1966 0.30 -0.1 2,080,288 - Psest_1966 0.30 -1.2 2,080,320 + Psest_1966 0.33 -1.0 2,080,320 + Psest_1966 0.33 -1.0 2,080,320 + Psest_1966 0.33 +0.6 2,080,332 + Psest_1966 0.34 +0.3 2,080,468 - Psest_1966 0.44 -0.5 2,080,468 - Psest_1966 0.44 -0.1 2,080,519 + Psest_1966 0.48 -0.2 2,080,520 - Psest_1966 0.48 -1.4 2,080,697 + Psest_1966 0.62 -0.7 2,080,705 + Psest_1966 0.63 -1.5 2,080,706 - Psest_1966 0.63 +1.1 2,080,802 + Psest_1966 0.70 -1.7 2,080,802 + Psest_1966 0.70 -0.6 2,080,803 - Psest_1966 0.70 -0.5 2,080,831 + Psest_1966 0.73 -0.1 2,080,836 + Psest_1966 0.73 +1.9 2,080,837 - Psest_1966 0.73 -1.3 2,080,945 + Psest_1966 0.81 -0.6 2,080,947 + Psest_1966 0.82 +1.2 2,080,947 + Psest_1966 0.82 -1.0 2,080,947 + Psest_1966 0.82 -1.3 2,080,948 - Psest_1966 0.82 +0.4 2,080,948 - Psest_1966 0.82 -2.5 2,080,948 - Psest_1966 0.82 -0.0 2,080,948 - Psest_1966 0.82 -0.9 2,080,951 + Psest_1966 0.82 +0.2 2,080,951 + Psest_1966 0.82 +1.7 2,080,951 + Psest_1966 0.82 +0.4 2,080,951 + Psest_1966 0.82 -0.6 2,080,952 - Psest_1966 0.82 -1.3 2,081,067 + -0.5 2,081,151 + -0.7 2,081,151 + +1.1 2,081,154 + -1.0 2,081,154 + +0.5 2,081,154 + -0.3 2,081,154 + +0.5 2,081,155 - -1.6 2,081,155 - -1.3 2,081,155 - -0.7 2,081,155 - +1.1 2,081,155 - -0.4 2,081,182 + -0.3 2,081,183 - -1.5 2,081,189 + +0.3 2,081,261 - -1.3 2,081,261 - -2.0 2,081,261 - -0.0 2,081,264 + +0.4 2,081,264 + +1.0 2,081,283 + -1.0 2,081,295 - -2.1 2,081,629 + -2.0 2,081,642 + -0.0
Or see this region's nucleotide sequence