Experiment: Choline chloride 90 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0723 and Psest_0724 are separated by 156 nucleotides Psest_0724 and Psest_0725 are separated by 104 nucleotides Psest_0725 and Psest_0726 are separated by 16 nucleotides
Psest_0723: Psest_0723 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 792,717 to 793,706
_0723
Psest_0724: Psest_0724 - Deoxyinosine 3'endonuclease (endonuclease V), at 793,863 to 794,591
_0724
Psest_0725: Psest_0725 - Negative regulator of beta-lactamase expression, at 794,696 to 795,247
_0725
Psest_0726: Psest_0726 - Membrane protein required for beta-lactamase induction, at 795,264 to 796,100
_0726
Position (kb) 794
795
796 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 793.714 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 794.008 kb on - strand, within Psest_0724 at 794.121 kb on + strand, within Psest_0724 at 794.121 kb on + strand, within Psest_0724 at 794.121 kb on + strand, within Psest_0724 at 794.121 kb on + strand, within Psest_0724 at 794.122 kb on - strand, within Psest_0724 at 794.122 kb on - strand, within Psest_0724 at 794.266 kb on + strand, within Psest_0724 at 794.395 kb on + strand, within Psest_0724 at 794.605 kb on + strand at 794.781 kb on + strand, within Psest_0725 at 794.781 kb on + strand, within Psest_0725 at 794.793 kb on + strand, within Psest_0725 at 794.794 kb on - strand, within Psest_0725 at 794.794 kb on - strand, within Psest_0725 at 795.055 kb on + strand, within Psest_0725 at 795.238 kb on + strand at 795.239 kb on - strand at 795.239 kb on - strand at 795.415 kb on + strand, within Psest_0726 at 795.519 kb on + strand, within Psest_0726 at 795.519 kb on + strand, within Psest_0726 at 795.520 kb on - strand, within Psest_0726 at 795.520 kb on - strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.854 kb on - strand, within Psest_0726 at 795.859 kb on + strand, within Psest_0726 at 795.934 kb on + strand, within Psest_0726 at 795.934 kb on + strand, within Psest_0726 at 795.934 kb on + strand, within Psest_0726 at 795.934 kb on + strand, within Psest_0726 at 795.934 kb on + strand, within Psest_0726 at 795.935 kb on - strand, within Psest_0726 at 795.935 kb on - strand, within Psest_0726 at 796.045 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 90 mM remove 793,714 + +1.8 793,718 + +0.5 793,718 + -0.4 793,718 + +1.9 793,718 + -1.7 793,718 + -0.5 793,718 + +0.3 793,718 + -0.5 793,718 + -1.1 793,718 + -1.2 793,718 + -1.2 793,719 - +2.2 793,719 - +0.6 793,719 - -1.3 793,719 - -1.8 793,719 - +0.2 793,719 - +0.1 793,719 - -2.0 793,719 - -2.3 794,008 - Psest_0724 0.20 +0.7 794,121 + Psest_0724 0.35 -0.3 794,121 + Psest_0724 0.35 +0.3 794,121 + Psest_0724 0.35 +0.9 794,121 + Psest_0724 0.35 -1.1 794,122 - Psest_0724 0.36 -1.9 794,122 - Psest_0724 0.36 +0.9 794,266 + Psest_0724 0.55 +1.5 794,395 + Psest_0724 0.73 +1.0 794,605 + +0.0 794,781 + Psest_0725 0.15 +0.3 794,781 + Psest_0725 0.15 -1.5 794,793 + Psest_0725 0.18 -0.1 794,794 - Psest_0725 0.18 -0.8 794,794 - Psest_0725 0.18 +0.3 795,055 + Psest_0725 0.65 -0.7 795,238 + +1.5 795,239 - -2.4 795,239 - -0.5 795,415 + Psest_0726 0.18 -2.0 795,519 + Psest_0726 0.30 -0.7 795,519 + Psest_0726 0.30 -0.3 795,520 - Psest_0726 0.31 -1.1 795,520 - Psest_0726 0.31 +0.2 795,690 + Psest_0726 0.51 -2.0 795,690 + Psest_0726 0.51 +0.1 795,690 + Psest_0726 0.51 -0.0 795,690 + Psest_0726 0.51 -0.3 795,690 + Psest_0726 0.51 -1.0 795,690 + Psest_0726 0.51 +1.9 795,690 + Psest_0726 0.51 -1.9 795,690 + Psest_0726 0.51 +0.4 795,690 + Psest_0726 0.51 +0.6 795,690 + Psest_0726 0.51 -1.4 795,690 + Psest_0726 0.51 -0.7 795,691 - Psest_0726 0.51 -1.4 795,691 - Psest_0726 0.51 +1.9 795,691 - Psest_0726 0.51 +0.6 795,691 - Psest_0726 0.51 +1.4 795,691 - Psest_0726 0.51 -0.0 795,691 - Psest_0726 0.51 -1.1 795,691 - Psest_0726 0.51 +0.7 795,854 - Psest_0726 0.70 -1.6 795,859 + Psest_0726 0.71 +0.2 795,934 + Psest_0726 0.80 -2.0 795,934 + Psest_0726 0.80 +0.2 795,934 + Psest_0726 0.80 -3.0 795,934 + Psest_0726 0.80 -2.0 795,934 + Psest_0726 0.80 +0.4 795,935 - Psest_0726 0.80 -0.4 795,935 - Psest_0726 0.80 -2.3 796,045 + -0.9
Or see this region's nucleotide sequence