Experiment: Choline chloride 90 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0189 and Psest_0190 are separated by 2 nucleotides Psest_0190 and Psest_0191 are separated by 21 nucleotides Psest_0191 and Psest_0192 overlap by 4 nucleotides
Psest_0189: Psest_0189 - Negative regulator of beta-lactamase expression, at 185,255 to 186,043
_0189
Psest_0190: Psest_0190 - Uncharacterized conserved protein, at 186,046 to 186,450
_0190
Psest_0191: Psest_0191 - PAS domain S-box, at 186,472 to 188,259
_0191
Psest_0192: Psest_0192 - Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains, at 188,256 to 189,602
_0192
Position (kb) 186
187
188
189 Strain fitness (log2 ratio) -2
-1
0
1
2 at 185.864 kb on + strand, within Psest_0189 at 185.961 kb on - strand, within Psest_0189 at 186.051 kb on - strand at 186.293 kb on + strand, within Psest_0190 at 186.293 kb on + strand, within Psest_0190 at 186.354 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.491 kb on + strand at 186.491 kb on + strand at 186.491 kb on + strand at 186.491 kb on + strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.565 kb on + strand at 186.645 kb on - strand at 186.735 kb on + strand, within Psest_0191 at 187.799 kb on + strand, within Psest_0191 at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.090 kb on - strand at 188.259 kb on + strand at 188.260 kb on - strand at 188.260 kb on - strand at 188.260 kb on - strand at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.686 kb on + strand, within Psest_0192 at 188.687 kb on - strand, within Psest_0192 at 188.800 kb on + strand, within Psest_0192 at 188.982 kb on + strand, within Psest_0192
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 90 mM remove 185,864 + Psest_0189 0.77 -1.9 185,961 - Psest_0189 0.89 -0.8 186,051 - -1.8 186,293 + Psest_0190 0.61 +0.2 186,293 + Psest_0190 0.61 +0.2 186,354 + Psest_0190 0.76 +0.4 186,356 + Psest_0190 0.77 -0.5 186,356 + Psest_0190 0.77 +1.4 186,356 + Psest_0190 0.77 -1.3 186,491 + +0.2 186,491 + -0.8 186,491 + -0.8 186,491 + -1.4 186,492 - -0.1 186,492 - -0.1 186,492 - +1.6 186,492 - -1.9 186,492 - +1.1 186,492 - +0.6 186,492 - +0.7 186,492 - -1.4 186,492 - -1.0 186,565 + -1.5 186,645 - +0.3 186,735 + Psest_0191 0.15 -0.4 187,799 + Psest_0191 0.74 -0.8 188,089 + +2.1 188,089 + +1.2 188,089 + +0.1 188,089 + +1.2 188,089 + -0.2 188,089 + -0.4 188,090 - -1.3 188,259 + +0.1 188,260 - -0.9 188,260 - -1.7 188,260 - -0.6 188,530 + Psest_0192 0.20 -1.2 188,530 + Psest_0192 0.20 +0.3 188,530 + Psest_0192 0.20 +0.2 188,530 + Psest_0192 0.20 -0.6 188,531 - Psest_0192 0.20 +0.0 188,531 - Psest_0192 0.20 -1.9 188,531 - Psest_0192 0.20 -0.3 188,686 + Psest_0192 0.32 +0.7 188,687 - Psest_0192 0.32 +0.3 188,800 + Psest_0192 0.40 -0.4 188,982 + Psest_0192 0.54 -1.0
Or see this region's nucleotide sequence