Experiment: Choline chloride 90 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1545 and Psest_1546 are separated by 95 nucleotides Psest_1546 and Psest_1547 are separated by 32 nucleotides Psest_1547 and Psest_1548 are separated by 125 nucleotides
Psest_1545: Psest_1545 - Anaerobic dehydrogenases, typically selenocysteine-containing, at 1,644,409 to 1,646,502
_1545
Psest_1546: Psest_1546 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 1,646,598 to 1,647,707
_1546
Psest_1547: Psest_1547 - Uncharacterized protein conserved in bacteria, at 1,647,740 to 1,648,456
_1547
Psest_1548: Psest_1548 - peptide chain release factor 2, at 1,648,582 to 1,649,677
_1548
Position (kb)
1646
1647
1648 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1645.657 kb on + strand, within Psest_1545 at 1645.657 kb on + strand, within Psest_1545 at 1645.657 kb on + strand, within Psest_1545 at 1645.658 kb on - strand, within Psest_1545 at 1645.658 kb on - strand, within Psest_1545 at 1645.658 kb on - strand, within Psest_1545 at 1645.679 kb on - strand, within Psest_1545 at 1645.679 kb on - strand, within Psest_1545 at 1645.727 kb on - strand, within Psest_1545 at 1645.727 kb on - strand, within Psest_1545 at 1645.759 kb on - strand, within Psest_1545 at 1645.995 kb on - strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.147 kb on + strand, within Psest_1545 at 1646.148 kb on - strand, within Psest_1545 at 1646.176 kb on + strand, within Psest_1545 at 1646.176 kb on + strand, within Psest_1545 at 1646.301 kb on - strand at 1646.301 kb on - strand at 1646.301 kb on - strand at 1646.451 kb on - strand at 1646.588 kb on - strand at 1646.792 kb on + strand, within Psest_1546 at 1646.792 kb on + strand, within Psest_1546 at 1646.792 kb on + strand, within Psest_1546 at 1646.793 kb on - strand, within Psest_1546 at 1646.793 kb on - strand, within Psest_1546 at 1646.913 kb on - strand, within Psest_1546 at 1646.913 kb on - strand, within Psest_1546 at 1646.913 kb on - strand, within Psest_1546 at 1647.124 kb on + strand, within Psest_1546 at 1647.124 kb on + strand, within Psest_1546 at 1647.124 kb on + strand, within Psest_1546 at 1647.125 kb on - strand, within Psest_1546 at 1647.125 kb on - strand, within Psest_1546 at 1647.648 kb on + strand at 1647.648 kb on + strand at 1647.648 kb on + strand at 1647.648 kb on + strand at 1647.648 kb on + strand at 1647.648 kb on + strand at 1647.649 kb on - strand at 1647.649 kb on - strand at 1647.649 kb on - strand at 1647.706 kb on - strand at 1647.706 kb on - strand at 1647.717 kb on - strand at 1647.983 kb on + strand, within Psest_1547 at 1647.983 kb on + strand, within Psest_1547 at 1647.984 kb on - strand, within Psest_1547 at 1647.984 kb on - strand, within Psest_1547 at 1648.017 kb on - strand, within Psest_1547 at 1648.017 kb on - strand, within Psest_1547 at 1648.319 kb on + strand, within Psest_1547 at 1648.319 kb on + strand, within Psest_1547 at 1648.319 kb on + strand, within Psest_1547 at 1648.319 kb on + strand, within Psest_1547 at 1648.319 kb on + strand, within Psest_1547 at 1648.319 kb on + strand, within Psest_1547 at 1648.320 kb on - strand, within Psest_1547 at 1648.320 kb on - strand, within Psest_1547 at 1648.320 kb on - strand, within Psest_1547 at 1648.320 kb on - strand, within Psest_1547 at 1648.320 kb on - strand, within Psest_1547 at 1648.320 kb on - strand, within Psest_1547 at 1648.320 kb on - strand, within Psest_1547 at 1648.320 kb on - strand, within Psest_1547 at 1648.320 kb on - strand, within Psest_1547 at 1648.320 kb on - strand, within Psest_1547 at 1648.437 kb on + strand at 1648.465 kb on + strand at 1648.466 kb on - strand at 1648.466 kb on - strand at 1648.537 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 90 mM remove 1,645,657 + Psest_1545 0.60 -0.1 1,645,657 + Psest_1545 0.60 -2.2 1,645,657 + Psest_1545 0.60 +0.1 1,645,658 - Psest_1545 0.60 +0.8 1,645,658 - Psest_1545 0.60 +0.2 1,645,658 - Psest_1545 0.60 -0.1 1,645,679 - Psest_1545 0.61 +1.6 1,645,679 - Psest_1545 0.61 -1.8 1,645,727 - Psest_1545 0.63 -0.6 1,645,727 - Psest_1545 0.63 -2.3 1,645,759 - Psest_1545 0.64 -0.3 1,645,995 - Psest_1545 0.76 -0.4 1,646,147 + Psest_1545 0.83 +0.1 1,646,147 + Psest_1545 0.83 -0.6 1,646,147 + Psest_1545 0.83 +2.5 1,646,147 + Psest_1545 0.83 -0.6 1,646,147 + Psest_1545 0.83 +1.7 1,646,147 + Psest_1545 0.83 +0.4 1,646,148 - Psest_1545 0.83 +0.0 1,646,176 + Psest_1545 0.84 +1.1 1,646,176 + Psest_1545 0.84 -0.7 1,646,301 - -0.1 1,646,301 - -0.8 1,646,301 - +0.1 1,646,451 - +1.5 1,646,588 - -3.5 1,646,792 + Psest_1546 0.17 +0.4 1,646,792 + Psest_1546 0.17 -0.6 1,646,792 + Psest_1546 0.17 -0.2 1,646,793 - Psest_1546 0.18 -0.1 1,646,793 - Psest_1546 0.18 -1.8 1,646,913 - Psest_1546 0.28 -0.5 1,646,913 - Psest_1546 0.28 +1.1 1,646,913 - Psest_1546 0.28 -2.3 1,647,124 + Psest_1546 0.47 -0.1 1,647,124 + Psest_1546 0.47 +0.2 1,647,124 + Psest_1546 0.47 -1.2 1,647,125 - Psest_1546 0.47 -1.1 1,647,125 - Psest_1546 0.47 -0.6 1,647,648 + +0.3 1,647,648 + +1.3 1,647,648 + +1.9 1,647,648 + +0.4 1,647,648 + -0.9 1,647,648 + -1.2 1,647,649 - +1.0 1,647,649 - +0.9 1,647,649 - +0.0 1,647,706 - -0.4 1,647,706 - +0.5 1,647,717 - -1.3 1,647,983 + Psest_1547 0.34 -1.6 1,647,983 + Psest_1547 0.34 -0.1 1,647,984 - Psest_1547 0.34 -1.4 1,647,984 - Psest_1547 0.34 +0.3 1,648,017 - Psest_1547 0.39 -0.3 1,648,017 - Psest_1547 0.39 +0.4 1,648,319 + Psest_1547 0.81 -0.4 1,648,319 + Psest_1547 0.81 -1.0 1,648,319 + Psest_1547 0.81 +0.2 1,648,319 + Psest_1547 0.81 -0.4 1,648,319 + Psest_1547 0.81 +0.0 1,648,319 + Psest_1547 0.81 +0.1 1,648,320 - Psest_1547 0.81 -0.8 1,648,320 - Psest_1547 0.81 +2.9 1,648,320 - Psest_1547 0.81 -0.7 1,648,320 - Psest_1547 0.81 -0.3 1,648,320 - Psest_1547 0.81 +1.1 1,648,320 - Psest_1547 0.81 -0.9 1,648,320 - Psest_1547 0.81 -0.8 1,648,320 - Psest_1547 0.81 -0.6 1,648,320 - Psest_1547 0.81 +0.4 1,648,320 - Psest_1547 0.81 +0.2 1,648,437 + -2.7 1,648,465 + +0.2 1,648,466 - -1.4 1,648,466 - -0.0 1,648,537 + -1.3
Or see this region's nucleotide sequence