Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0542

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0541 and Psest_0542 are separated by 220 nucleotidesPsest_0542 and Psest_0543 are separated by 80 nucleotidesPsest_0543 and Psest_0544 are separated by 15 nucleotidesPsest_0544 and Psest_0545 are separated by 76 nucleotides Psest_0541: Psest_0541 - succinate CoA transferases, at 596,161 to 597,672 _0541 Psest_0542: Psest_0542 - AMP nucleosidase, at 597,893 to 599,368 _0542 Psest_0543: Psest_0543 - Protein of unknown function (DUF2789)., at 599,449 to 599,691 _0543 Psest_0544: Psest_0544 - Hydrogenase/urease accessory protein, at 599,707 to 600,279 _0544 Psest_0545: Psest_0545 - urease accessory protein UreG, at 600,356 to 600,970 _0545 Position (kb) 597 598 599 600Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 597.158 kb on - strand, within Psest_0541at 597.158 kb on - strand, within Psest_0541at 597.158 kb on - strand, within Psest_0541at 597.262 kb on + strand, within Psest_0541at 597.262 kb on + strand, within Psest_0541at 597.263 kb on - strand, within Psest_0541at 597.263 kb on - strand, within Psest_0541at 597.263 kb on - strand, within Psest_0541at 597.348 kb on + strand, within Psest_0541at 597.348 kb on + strand, within Psest_0541at 597.348 kb on + strand, within Psest_0541at 597.348 kb on + strand, within Psest_0541at 597.349 kb on - strand, within Psest_0541at 597.349 kb on - strand, within Psest_0541at 597.349 kb on - strand, within Psest_0541at 597.349 kb on - strand, within Psest_0541at 597.469 kb on + strand, within Psest_0541at 597.470 kb on - strand, within Psest_0541at 597.470 kb on - strand, within Psest_0541at 597.562 kb on + strandat 597.670 kb on + strandat 597.670 kb on + strandat 597.670 kb on + strandat 597.670 kb on + strandat 597.670 kb on + strandat 597.671 kb on - strandat 597.671 kb on - strandat 597.835 kb on + strandat 597.836 kb on - strandat 597.837 kb on + strandat 597.837 kb on + strandat 597.837 kb on + strandat 597.837 kb on + strandat 597.837 kb on + strandat 597.837 kb on + strandat 597.837 kb on + strandat 597.837 kb on + strandat 597.837 kb on + strandat 597.838 kb on - strandat 597.838 kb on - strandat 597.838 kb on - strandat 597.838 kb on - strandat 597.838 kb on - strandat 597.838 kb on - strandat 597.838 kb on - strandat 597.838 kb on - strandat 597.865 kb on - strandat 597.865 kb on - strandat 597.877 kb on + strandat 597.888 kb on + strandat 598.010 kb on + strandat 598.011 kb on - strandat 598.011 kb on - strandat 598.015 kb on - strandat 598.083 kb on - strand, within Psest_0542at 598.083 kb on - strand, within Psest_0542at 598.122 kb on - strand, within Psest_0542at 598.148 kb on + strand, within Psest_0542at 598.148 kb on + strand, within Psest_0542at 598.275 kb on - strand, within Psest_0542at 598.275 kb on - strand, within Psest_0542at 598.275 kb on - strand, within Psest_0542at 598.275 kb on - strand, within Psest_0542at 598.375 kb on + strand, within Psest_0542at 598.392 kb on - strand, within Psest_0542at 598.475 kb on + strand, within Psest_0542at 598.475 kb on + strand, within Psest_0542at 598.475 kb on + strand, within Psest_0542at 598.476 kb on - strand, within Psest_0542at 598.476 kb on - strand, within Psest_0542at 598.500 kb on + strand, within Psest_0542at 598.500 kb on + strand, within Psest_0542at 598.500 kb on + strand, within Psest_0542at 598.501 kb on - strand, within Psest_0542at 598.501 kb on - strand, within Psest_0542at 598.526 kb on + strand, within Psest_0542at 598.526 kb on + strand, within Psest_0542at 598.527 kb on - strand, within Psest_0542at 598.527 kb on - strand, within Psest_0542at 598.798 kb on - strand, within Psest_0542at 598.865 kb on + strand, within Psest_0542at 598.865 kb on + strand, within Psest_0542at 598.865 kb on + strand, within Psest_0542at 598.866 kb on - strand, within Psest_0542at 598.883 kb on + strand, within Psest_0542at 598.883 kb on + strand, within Psest_0542at 598.883 kb on + strand, within Psest_0542at 598.883 kb on + strand, within Psest_0542at 598.883 kb on + strand, within Psest_0542at 598.884 kb on - strand, within Psest_0542at 598.884 kb on - strand, within Psest_0542at 598.884 kb on - strand, within Psest_0542at 598.884 kb on - strand, within Psest_0542at 598.884 kb on - strand, within Psest_0542at 599.171 kb on + strand, within Psest_0542at 599.246 kb on + strandat 599.246 kb on + strandat 599.247 kb on - strandat 599.247 kb on - strandat 599.247 kb on - strandat 599.408 kb on + strandat 599.409 kb on - strandat 599.415 kb on - strandat 599.415 kb on - strandat 599.415 kb on - strandat 599.415 kb on - strandat 599.475 kb on + strand, within Psest_0543at 599.563 kb on + strand, within Psest_0543at 600.124 kb on + strand, within Psest_0544at 600.125 kb on - strand, within Psest_0544at 600.271 kb on + strandat 600.271 kb on + strandat 600.271 kb on + strandat 600.271 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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597,158 - Psest_0541 0.66 -0.7
597,158 - Psest_0541 0.66 -0.6
597,158 - Psest_0541 0.66 -1.4
597,262 + Psest_0541 0.73 +1.5
597,262 + Psest_0541 0.73 +0.9
597,263 - Psest_0541 0.73 +1.8
597,263 - Psest_0541 0.73 +0.7
597,263 - Psest_0541 0.73 +0.5
597,348 + Psest_0541 0.79 -0.4
597,348 + Psest_0541 0.79 -0.1
597,348 + Psest_0541 0.79 +0.7
597,348 + Psest_0541 0.79 +1.4
597,349 - Psest_0541 0.79 -0.8
597,349 - Psest_0541 0.79 +0.8
597,349 - Psest_0541 0.79 -0.8
597,349 - Psest_0541 0.79 -0.8
597,469 + Psest_0541 0.87 -0.3
597,470 - Psest_0541 0.87 -2.8
597,470 - Psest_0541 0.87 +1.8
597,562 + +1.7
597,670 + +2.5
597,670 + -2.2
597,670 + -0.1
597,670 + +1.5
597,670 + -1.7
597,671 - +1.5
597,671 - -1.6
597,835 + +1.8
597,836 - +0.1
597,837 + +2.9
597,837 + +3.3
597,837 + +0.4
597,837 + +1.3
597,837 + -1.0
597,837 + +0.4
597,837 + -0.3
597,837 + -0.1
597,837 + +0.6
597,838 - -0.7
597,838 - -1.9
597,838 - -0.7
597,838 - +0.7
597,838 - -0.6
597,838 - +0.3
597,838 - +0.7
597,838 - +0.5
597,865 - +1.8
597,865 - -0.2
597,877 + -2.5
597,888 + +0.3
598,010 + -0.3
598,011 - -0.6
598,011 - +0.5
598,015 - +2.6
598,083 - Psest_0542 0.13 -0.9
598,083 - Psest_0542 0.13 -1.1
598,122 - Psest_0542 0.16 +0.5
598,148 + Psest_0542 0.17 -1.5
598,148 + Psest_0542 0.17 -0.3
598,275 - Psest_0542 0.26 -1.0
598,275 - Psest_0542 0.26 -0.4
598,275 - Psest_0542 0.26 -1.1
598,275 - Psest_0542 0.26 -2.6
598,375 + Psest_0542 0.33 -1.9
598,392 - Psest_0542 0.34 -1.7
598,475 + Psest_0542 0.39 -0.0
598,475 + Psest_0542 0.39 -0.0
598,475 + Psest_0542 0.39 -1.0
598,476 - Psest_0542 0.39 -0.2
598,476 - Psest_0542 0.39 -1.5
598,500 + Psest_0542 0.41 -0.5
598,500 + Psest_0542 0.41 -0.7
598,500 + Psest_0542 0.41 -1.8
598,501 - Psest_0542 0.41 -3.0
598,501 - Psest_0542 0.41 -1.0
598,526 + Psest_0542 0.43 -0.6
598,526 + Psest_0542 0.43 +0.0
598,527 - Psest_0542 0.43 -1.3
598,527 - Psest_0542 0.43 -0.3
598,798 - Psest_0542 0.61 +0.1
598,865 + Psest_0542 0.66 -2.4
598,865 + Psest_0542 0.66 -0.4
598,865 + Psest_0542 0.66 +0.9
598,866 - Psest_0542 0.66 +1.3
598,883 + Psest_0542 0.67 -0.7
598,883 + Psest_0542 0.67 -1.9
598,883 + Psest_0542 0.67 -1.1
598,883 + Psest_0542 0.67 -3.0
598,883 + Psest_0542 0.67 -0.6
598,884 - Psest_0542 0.67 -2.6
598,884 - Psest_0542 0.67 -1.9
598,884 - Psest_0542 0.67 -0.4
598,884 - Psest_0542 0.67 -2.0
598,884 - Psest_0542 0.67 -2.0
599,171 + Psest_0542 0.87 -1.3
599,246 + +0.7
599,246 + -0.4
599,247 - -1.5
599,247 - -0.5
599,247 - -1.5
599,408 + +0.3
599,409 - -0.5
599,415 - -1.0
599,415 - +0.1
599,415 - -2.4
599,415 - -0.0
599,475 + Psest_0543 0.11 +1.2
599,563 + Psest_0543 0.47 +0.6
600,124 + Psest_0544 0.73 -0.6
600,125 - Psest_0544 0.73 -2.6
600,271 + -0.1
600,271 + -1.3
600,271 + +2.1
600,271 + +1.5

Or see this region's nucleotide sequence