Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2024 and Psest_2025 are separated by 61 nucleotides Psest_2025 and Psest_2026 are separated by 218 nucleotides Psest_2026 and Psest_2027 are separated by 29 nucleotides Psest_2027 and Psest_2028 are separated by 124 nucleotides Psest_2028 and Psest_2029 are separated by 100 nucleotides
Psest_2024: Psest_2024 - isocitrate dehydrogenase, NADP-dependent, prokaryotic type, at 2,144,486 to 2,145,742
_2024
Psest_2025: Psest_2025 - cold shock domain protein CspD, at 2,145,804 to 2,146,064
_2025
Psest_2026: Psest_2026 - Uncharacterized conserved protein, at 2,146,283 to 2,146,645
_2026
Psest_2027: Psest_2027 - ATP-dependent Clp protease ATP-binding subunit clpA, at 2,146,675 to 2,148,945
_2027
Psest_2028: Psest_2028 - translation initiation factor IF-1, at 2,149,070 to 2,149,288
_2028
Psest_2029: Psest_2029 - Putative arginyl-tRNA:protein arginylyltransferase, at 2,149,389 to 2,150,096
_2029
Position (kb)
2146
2147
2148
2149 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.736 kb on + strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.737 kb on - strand at 2145.740 kb on + strand at 2145.741 kb on - strand at 2146.008 kb on + strand, within Psest_2025 at 2146.059 kb on + strand at 2146.059 kb on + strand at 2146.060 kb on - strand at 2146.060 kb on - strand at 2146.101 kb on + strand at 2146.101 kb on + strand at 2146.101 kb on + strand at 2146.124 kb on + strand at 2146.167 kb on + strand at 2146.168 kb on - strand at 2146.175 kb on + strand at 2146.175 kb on + strand at 2146.176 kb on - strand at 2146.176 kb on - strand at 2146.184 kb on + strand at 2146.185 kb on - strand at 2146.241 kb on - strand at 2146.245 kb on - strand at 2146.245 kb on - strand at 2146.245 kb on - strand at 2146.245 kb on - strand at 2146.268 kb on - strand at 2146.308 kb on + strand at 2146.308 kb on + strand at 2146.308 kb on + strand at 2146.403 kb on + strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.404 kb on - strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.405 kb on + strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.406 kb on - strand, within Psest_2026 at 2146.420 kb on + strand, within Psest_2026 at 2146.421 kb on - strand, within Psest_2026 at 2146.431 kb on - strand, within Psest_2026 at 2146.547 kb on + strand, within Psest_2026 at 2146.547 kb on + strand, within Psest_2026 at 2146.548 kb on - strand, within Psest_2026 at 2146.592 kb on + strand, within Psest_2026 at 2146.592 kb on + strand, within Psest_2026 at 2146.593 kb on - strand, within Psest_2026 at 2146.674 kb on + strand at 2146.674 kb on + strand at 2146.674 kb on + strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.675 kb on - strand at 2146.728 kb on + strand at 2146.728 kb on + strand at 2146.879 kb on - strand at 2147.083 kb on + strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.084 kb on - strand, within Psest_2027 at 2147.200 kb on + strand, within Psest_2027 at 2147.200 kb on + strand, within Psest_2027 at 2147.200 kb on + strand, within Psest_2027 at 2147.201 kb on - strand, within Psest_2027 at 2147.201 kb on - strand, within Psest_2027 at 2147.201 kb on - strand, within Psest_2027 at 2147.253 kb on - strand, within Psest_2027 at 2147.423 kb on - strand, within Psest_2027 at 2147.447 kb on + strand, within Psest_2027 at 2147.454 kb on + strand, within Psest_2027 at 2147.550 kb on + strand, within Psest_2027 at 2147.603 kb on + strand, within Psest_2027 at 2147.803 kb on + strand, within Psest_2027 at 2147.804 kb on - strand, within Psest_2027 at 2147.848 kb on + strand, within Psest_2027 at 2147.848 kb on + strand, within Psest_2027 at 2147.848 kb on + strand, within Psest_2027 at 2147.849 kb on - strand, within Psest_2027 at 2147.849 kb on - strand, within Psest_2027 at 2147.849 kb on - strand, within Psest_2027 at 2147.850 kb on + strand, within Psest_2027 at 2147.850 kb on + strand, within Psest_2027 at 2147.850 kb on + strand, within Psest_2027 at 2147.850 kb on + strand, within Psest_2027 at 2147.851 kb on - strand, within Psest_2027 at 2147.865 kb on + strand, within Psest_2027 at 2147.866 kb on - strand, within Psest_2027 at 2147.866 kb on - strand, within Psest_2027 at 2147.909 kb on - strand, within Psest_2027 at 2148.256 kb on + strand, within Psest_2027 at 2148.257 kb on - strand, within Psest_2027 at 2148.258 kb on + strand, within Psest_2027 at 2148.258 kb on + strand, within Psest_2027 at 2148.259 kb on - strand, within Psest_2027 at 2148.259 kb on - strand, within Psest_2027 at 2148.259 kb on - strand, within Psest_2027 at 2148.304 kb on + strand, within Psest_2027 at 2148.329 kb on + strand, within Psest_2027 at 2148.329 kb on + strand, within Psest_2027 at 2148.329 kb on + strand, within Psest_2027 at 2148.330 kb on - strand, within Psest_2027 at 2148.477 kb on + strand, within Psest_2027 at 2148.543 kb on + strand, within Psest_2027 at 2148.543 kb on + strand, within Psest_2027 at 2148.621 kb on + strand, within Psest_2027 at 2148.769 kb on + strand at 2148.769 kb on + strand at 2149.450 kb on + strand at 2149.451 kb on - strand at 2149.451 kb on - strand at 2149.451 kb on - strand at 2149.579 kb on + strand, within Psest_2029 at 2149.588 kb on + strand, within Psest_2029 at 2149.588 kb on + strand, within Psest_2029 at 2149.589 kb on - strand, within Psest_2029 at 2149.607 kb on + strand, within Psest_2029 at 2149.700 kb on + strand, within Psest_2029 at 2149.700 kb on + strand, within Psest_2029 at 2149.726 kb on + strand, within Psest_2029 at 2149.726 kb on + strand, within Psest_2029 at 2149.727 kb on - strand, within Psest_2029 at 2149.731 kb on - strand, within Psest_2029 at 2149.758 kb on - strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.759 kb on + strand, within Psest_2029 at 2149.760 kb on - strand, within Psest_2029 at 2149.760 kb on - strand, within Psest_2029 at 2149.760 kb on - strand, within Psest_2029 at 2149.760 kb on - strand, within Psest_2029 at 2149.760 kb on - strand, within Psest_2029 at 2149.768 kb on + strand, within Psest_2029 at 2149.768 kb on + strand, within Psest_2029 at 2149.768 kb on + strand, within Psest_2029 at 2149.769 kb on - strand, within Psest_2029 at 2149.769 kb on - strand, within Psest_2029 at 2149.775 kb on + strand, within Psest_2029 at 2149.775 kb on + strand, within Psest_2029 at 2149.776 kb on - strand, within Psest_2029 at 2149.777 kb on + strand, within Psest_2029 at 2149.778 kb on - strand, within Psest_2029
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 2,145,736 + -1.5 2,145,736 + -0.6 2,145,736 + +1.4 2,145,736 + -0.5 2,145,736 + +0.5 2,145,736 + +3.1 2,145,736 + -1.1 2,145,736 + -0.9 2,145,737 - +0.6 2,145,737 - -0.8 2,145,737 - -0.9 2,145,737 - +0.5 2,145,737 - +2.4 2,145,737 - +0.6 2,145,740 + +1.3 2,145,741 - -0.9 2,146,008 + Psest_2025 0.78 +2.3 2,146,059 + +1.1 2,146,059 + +1.3 2,146,060 - +1.0 2,146,060 - +0.5 2,146,101 + -0.2 2,146,101 + +0.0 2,146,101 + +0.3 2,146,124 + -0.3 2,146,167 + -2.0 2,146,168 - +0.7 2,146,175 + +1.1 2,146,175 + +1.5 2,146,176 - -0.8 2,146,176 - +1.3 2,146,184 + +0.4 2,146,185 - -1.4 2,146,241 - -1.1 2,146,245 - +1.0 2,146,245 - -0.0 2,146,245 - +0.5 2,146,245 - +0.7 2,146,268 - -0.3 2,146,308 + -1.1 2,146,308 + +0.6 2,146,308 + +1.2 2,146,403 + Psest_2026 0.33 -0.1 2,146,404 - Psest_2026 0.33 -1.1 2,146,404 - Psest_2026 0.33 -0.2 2,146,404 - Psest_2026 0.33 -2.0 2,146,404 - Psest_2026 0.33 -0.0 2,146,404 - Psest_2026 0.33 -0.6 2,146,404 - Psest_2026 0.33 -0.4 2,146,405 + Psest_2026 0.34 +0.8 2,146,405 + Psest_2026 0.34 +0.7 2,146,405 + Psest_2026 0.34 +0.4 2,146,405 + Psest_2026 0.34 +0.2 2,146,405 + Psest_2026 0.34 +1.7 2,146,405 + Psest_2026 0.34 +2.4 2,146,405 + Psest_2026 0.34 +1.3 2,146,406 - Psest_2026 0.34 +0.7 2,146,406 - Psest_2026 0.34 +0.5 2,146,406 - Psest_2026 0.34 -1.3 2,146,406 - Psest_2026 0.34 -0.1 2,146,406 - Psest_2026 0.34 -0.2 2,146,406 - Psest_2026 0.34 -1.4 2,146,406 - Psest_2026 0.34 +1.2 2,146,406 - Psest_2026 0.34 -1.7 2,146,406 - Psest_2026 0.34 -0.4 2,146,420 + Psest_2026 0.38 +1.5 2,146,421 - Psest_2026 0.38 -0.9 2,146,431 - Psest_2026 0.41 -1.5 2,146,547 + Psest_2026 0.73 +1.4 2,146,547 + Psest_2026 0.73 +0.7 2,146,548 - Psest_2026 0.73 -0.3 2,146,592 + Psest_2026 0.85 +0.7 2,146,592 + Psest_2026 0.85 +0.4 2,146,593 - Psest_2026 0.85 -0.0 2,146,674 + +1.3 2,146,674 + +1.1 2,146,674 + -1.6 2,146,675 - -0.6 2,146,675 - +0.1 2,146,675 - +1.8 2,146,675 - +2.2 2,146,675 - -1.2 2,146,675 - -0.4 2,146,728 + +0.5 2,146,728 + -1.2 2,146,879 - -0.4 2,147,083 + Psest_2027 0.18 -0.0 2,147,084 - Psest_2027 0.18 +1.8 2,147,084 - Psest_2027 0.18 +0.6 2,147,084 - Psest_2027 0.18 +1.4 2,147,084 - Psest_2027 0.18 +0.8 2,147,084 - Psest_2027 0.18 +0.6 2,147,084 - Psest_2027 0.18 -0.8 2,147,200 + Psest_2027 0.23 +0.6 2,147,200 + Psest_2027 0.23 +0.7 2,147,200 + Psest_2027 0.23 -0.4 2,147,201 - Psest_2027 0.23 -0.3 2,147,201 - Psest_2027 0.23 +0.2 2,147,201 - Psest_2027 0.23 -1.7 2,147,253 - Psest_2027 0.25 +0.5 2,147,423 - Psest_2027 0.33 -0.5 2,147,447 + Psest_2027 0.34 +1.1 2,147,454 + Psest_2027 0.34 -1.3 2,147,550 + Psest_2027 0.39 -1.1 2,147,603 + Psest_2027 0.41 -0.4 2,147,803 + Psest_2027 0.50 -0.7 2,147,804 - Psest_2027 0.50 -0.3 2,147,848 + Psest_2027 0.52 +0.6 2,147,848 + Psest_2027 0.52 +2.0 2,147,848 + Psest_2027 0.52 +0.8 2,147,849 - Psest_2027 0.52 -1.0 2,147,849 - Psest_2027 0.52 -1.8 2,147,849 - Psest_2027 0.52 +0.0 2,147,850 + Psest_2027 0.52 -1.1 2,147,850 + Psest_2027 0.52 +1.8 2,147,850 + Psest_2027 0.52 -0.2 2,147,850 + Psest_2027 0.52 +0.3 2,147,851 - Psest_2027 0.52 -0.8 2,147,865 + Psest_2027 0.52 -1.0 2,147,866 - Psest_2027 0.52 +0.4 2,147,866 - Psest_2027 0.52 -0.3 2,147,909 - Psest_2027 0.54 -0.5 2,148,256 + Psest_2027 0.70 -1.1 2,148,257 - Psest_2027 0.70 +0.2 2,148,258 + Psest_2027 0.70 +0.5 2,148,258 + Psest_2027 0.70 +0.7 2,148,259 - Psest_2027 0.70 -0.8 2,148,259 - Psest_2027 0.70 -1.5 2,148,259 - Psest_2027 0.70 -0.7 2,148,304 + Psest_2027 0.72 +0.2 2,148,329 + Psest_2027 0.73 +0.5 2,148,329 + Psest_2027 0.73 +0.0 2,148,329 + Psest_2027 0.73 -1.9 2,148,330 - Psest_2027 0.73 +1.8 2,148,477 + Psest_2027 0.79 -2.0 2,148,543 + Psest_2027 0.82 +1.0 2,148,543 + Psest_2027 0.82 -0.8 2,148,621 + Psest_2027 0.86 +0.5 2,148,769 + +1.3 2,148,769 + -0.4 2,149,450 + +0.4 2,149,451 - -0.1 2,149,451 - +1.7 2,149,451 - +0.9 2,149,579 + Psest_2029 0.27 -0.0 2,149,588 + Psest_2029 0.28 +0.7 2,149,588 + Psest_2029 0.28 -0.4 2,149,589 - Psest_2029 0.28 -1.8 2,149,607 + Psest_2029 0.31 +0.3 2,149,700 + Psest_2029 0.44 +0.2 2,149,700 + Psest_2029 0.44 -2.4 2,149,726 + Psest_2029 0.48 -0.8 2,149,726 + Psest_2029 0.48 +0.7 2,149,727 - Psest_2029 0.48 +0.2 2,149,731 - Psest_2029 0.48 -0.3 2,149,758 - Psest_2029 0.52 +2.4 2,149,759 + Psest_2029 0.52 +1.9 2,149,759 + Psest_2029 0.52 -1.8 2,149,759 + Psest_2029 0.52 -1.8 2,149,759 + Psest_2029 0.52 +1.0 2,149,759 + Psest_2029 0.52 +1.0 2,149,759 + Psest_2029 0.52 -1.4 2,149,759 + Psest_2029 0.52 -0.1 2,149,759 + Psest_2029 0.52 +0.4 2,149,759 + Psest_2029 0.52 +1.4 2,149,760 - Psest_2029 0.52 +0.3 2,149,760 - Psest_2029 0.52 -1.3 2,149,760 - Psest_2029 0.52 +0.7 2,149,760 - Psest_2029 0.52 +2.0 2,149,760 - Psest_2029 0.52 +0.5 2,149,768 + Psest_2029 0.54 -0.4 2,149,768 + Psest_2029 0.54 +0.9 2,149,768 + Psest_2029 0.54 -1.3 2,149,769 - Psest_2029 0.54 -0.5 2,149,769 - Psest_2029 0.54 +0.2 2,149,775 + Psest_2029 0.55 +0.3 2,149,775 + Psest_2029 0.55 +1.2 2,149,776 - Psest_2029 0.55 -1.5 2,149,777 + Psest_2029 0.55 -0.4 2,149,778 - Psest_2029 0.55 +0.5
Or see this region's nucleotide sequence