Experiment: PEG (Day 3) - Cage1;Mouse2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2330 and BT2331 are separated by 5 nucleotides BT2331 and BT2332 are separated by 254 nucleotides BT2332 and BT2333 are separated by 12 nucleotides BT2333 and BT2334 overlap by 17 nucleotides
BT2330: BT2330 - hypothetical protein (NCBI ptt file), at 2,911,335 to 2,914,052
BT2330
BT2331: BT2331 - conserved hypothetical protein, with a calcineurin-like phosphoesterase domain (NCBI ptt file), at 2,914,058 to 2,914,375
BT2331
BT2332: BT2332 - conserved hypothetical protein (NCBI ptt file), at 2,914,630 to 2,914,983
BT2332
BT2333: BT2333 - conserved hypothetical protein (NCBI ptt file), at 2,914,996 to 2,917,041
BT2333
BT2334: BT2334 - conserved hypothetical protein (NCBI ptt file), at 2,917,025 to 2,917,369
BT2334
Position (kb)
2914
2915
2916
2917
2918 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2914.015 kb on - strand at 2914.015 kb on - strand at 2914.017 kb on - strand at 2914.017 kb on - strand at 2914.023 kb on - strand at 2914.023 kb on - strand at 2914.023 kb on - strand at 2914.025 kb on + strand at 2914.037 kb on + strand at 2914.037 kb on + strand at 2914.039 kb on + strand at 2914.045 kb on + strand at 2914.046 kb on - strand at 2914.046 kb on - strand at 2914.059 kb on + strand at 2914.059 kb on + strand at 2914.150 kb on - strand, within BT2331 at 2914.164 kb on - strand, within BT2331 at 2914.203 kb on + strand, within BT2331 at 2914.203 kb on + strand, within BT2331 at 2914.225 kb on - strand, within BT2331 at 2914.240 kb on + strand, within BT2331 at 2914.241 kb on - strand, within BT2331 at 2914.247 kb on + strand, within BT2331 at 2914.247 kb on + strand, within BT2331 at 2914.248 kb on - strand, within BT2331 at 2914.248 kb on - strand, within BT2331 at 2914.249 kb on + strand, within BT2331 at 2914.249 kb on + strand, within BT2331 at 2914.249 kb on + strand, within BT2331 at 2914.250 kb on - strand, within BT2331 at 2914.250 kb on - strand, within BT2331 at 2914.250 kb on - strand, within BT2331 at 2914.251 kb on + strand, within BT2331 at 2914.254 kb on - strand, within BT2331 at 2914.272 kb on + strand, within BT2331 at 2914.337 kb on + strand, within BT2331 at 2914.338 kb on - strand, within BT2331 at 2914.358 kb on + strand at 2914.358 kb on + strand at 2914.359 kb on - strand at 2914.359 kb on - strand at 2914.376 kb on - strand at 2914.434 kb on + strand at 2914.448 kb on + strand at 2914.448 kb on + strand at 2914.449 kb on - strand at 2914.453 kb on - strand at 2914.490 kb on + strand at 2914.491 kb on - strand at 2914.537 kb on + strand at 2914.537 kb on + strand at 2914.537 kb on + strand at 2914.561 kb on + strand at 2914.567 kb on - strand at 2914.611 kb on - strand at 2914.742 kb on + strand, within BT2332 at 2914.743 kb on - strand, within BT2332 at 2914.745 kb on + strand, within BT2332 at 2914.746 kb on - strand, within BT2332 at 2914.746 kb on - strand, within BT2332 at 2914.759 kb on + strand, within BT2332 at 2914.834 kb on + strand, within BT2332 at 2914.834 kb on + strand, within BT2332 at 2914.835 kb on - strand, within BT2332 at 2914.872 kb on - strand, within BT2332 at 2914.872 kb on - strand, within BT2332 at 2914.941 kb on + strand, within BT2332 at 2914.942 kb on - strand, within BT2332 at 2914.978 kb on + strand at 2914.983 kb on + strand at 2914.983 kb on + strand at 2914.983 kb on + strand at 2915.015 kb on + strand at 2915.138 kb on + strand at 2915.142 kb on + strand at 2915.280 kb on + strand, within BT2333 at 2915.280 kb on + strand, within BT2333 at 2915.280 kb on + strand, within BT2333 at 2915.440 kb on + strand, within BT2333 at 2915.462 kb on + strand, within BT2333 at 2915.582 kb on + strand, within BT2333 at 2915.588 kb on + strand, within BT2333 at 2915.651 kb on + strand, within BT2333 at 2915.717 kb on + strand, within BT2333 at 2915.725 kb on + strand, within BT2333 at 2915.852 kb on + strand, within BT2333 at 2915.886 kb on + strand, within BT2333 at 2915.886 kb on + strand, within BT2333 at 2916.074 kb on + strand, within BT2333 at 2916.197 kb on + strand, within BT2333 at 2916.198 kb on - strand, within BT2333 at 2916.210 kb on + strand, within BT2333 at 2916.383 kb on + strand, within BT2333 at 2916.447 kb on + strand, within BT2333 at 2916.447 kb on + strand, within BT2333 at 2916.462 kb on + strand, within BT2333 at 2916.465 kb on - strand, within BT2333 at 2916.639 kb on + strand, within BT2333 at 2916.641 kb on + strand, within BT2333 at 2916.719 kb on + strand, within BT2333 at 2916.719 kb on + strand, within BT2333 at 2916.720 kb on - strand, within BT2333 at 2916.848 kb on + strand at 2916.849 kb on - strand at 2916.902 kb on + strand at 2916.930 kb on + strand at 2917.024 kb on - strand at 2917.105 kb on + strand, within BT2334 at 2917.105 kb on + strand, within BT2334 at 2917.147 kb on + strand, within BT2334 at 2917.147 kb on + strand, within BT2334 at 2917.207 kb on + strand, within BT2334 at 2917.207 kb on + strand, within BT2334 at 2917.207 kb on + strand, within BT2334 at 2917.207 kb on + strand, within BT2334 at 2917.207 kb on - strand, within BT2334 at 2917.213 kb on + strand, within BT2334 at 2917.213 kb on + strand, within BT2334 at 2917.214 kb on - strand, within BT2334 at 2917.295 kb on + strand, within BT2334 at 2917.338 kb on + strand at 2917.361 kb on - strand at 2917.369 kb on + strand at 2917.369 kb on + strand at 2917.411 kb on + strand at 2917.411 kb on + strand at 2917.431 kb on - strand at 2917.433 kb on + strand at 2917.434 kb on - strand at 2917.435 kb on + strand at 2917.435 kb on + strand at 2917.435 kb on + strand at 2917.435 kb on + strand at 2917.435 kb on + strand at 2917.436 kb on - strand at 2917.444 kb on + strand at 2917.444 kb on + strand at 2917.445 kb on - strand at 2917.574 kb on + strand at 2917.574 kb on + strand at 2917.574 kb on + strand at 2917.574 kb on + strand at 2917.574 kb on + strand at 2917.575 kb on - strand at 2917.575 kb on - strand at 2917.575 kb on - strand at 2917.576 kb on + strand at 2917.623 kb on + strand at 2917.720 kb on - strand at 2917.720 kb on - strand at 2917.729 kb on + strand at 2917.732 kb on + strand at 2917.733 kb on - strand at 2917.746 kb on - strand at 2917.746 kb on - strand at 2917.774 kb on + strand at 2917.775 kb on - strand at 2917.775 kb on - strand at 2917.785 kb on + strand at 2917.917 kb on + strand at 2917.996 kb on - strand at 2918.013 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PEG (Day 3) - Cage1;Mouse2 remove 2,914,015 - +0.6 2,914,015 - -1.3 2,914,017 - -0.9 2,914,017 - +1.7 2,914,023 - -0.9 2,914,023 - -0.4 2,914,023 - -1.9 2,914,025 + -1.3 2,914,037 + -1.5 2,914,037 + +3.2 2,914,039 + -0.7 2,914,045 + +1.7 2,914,046 - +0.2 2,914,046 - -1.1 2,914,059 + -1.1 2,914,059 + +0.2 2,914,150 - BT2331 0.29 +0.1 2,914,164 - BT2331 0.33 -2.6 2,914,203 + BT2331 0.46 -1.1 2,914,203 + BT2331 0.46 -1.5 2,914,225 - BT2331 0.53 -0.3 2,914,240 + BT2331 0.57 -2.5 2,914,241 - BT2331 0.58 +0.9 2,914,247 + BT2331 0.59 +1.0 2,914,247 + BT2331 0.59 -1.1 2,914,248 - BT2331 0.60 +0.7 2,914,248 - BT2331 0.60 -1.0 2,914,249 + BT2331 0.60 -2.5 2,914,249 + BT2331 0.60 -1.3 2,914,249 + BT2331 0.60 -2.0 2,914,250 - BT2331 0.60 +1.2 2,914,250 - BT2331 0.60 -0.3 2,914,250 - BT2331 0.60 +1.7 2,914,251 + BT2331 0.61 -1.1 2,914,254 - BT2331 0.62 -1.3 2,914,272 + BT2331 0.67 -0.8 2,914,337 + BT2331 0.88 -0.4 2,914,338 - BT2331 0.88 +0.1 2,914,358 + -1.0 2,914,358 + -0.9 2,914,359 - -0.6 2,914,359 - +0.2 2,914,376 - +0.1 2,914,434 + -3.2 2,914,448 + -1.7 2,914,448 + -1.5 2,914,449 - -0.3 2,914,453 - +0.1 2,914,490 + -0.7 2,914,491 - -0.8 2,914,537 + -0.7 2,914,537 + -1.5 2,914,537 + -0.6 2,914,561 + -0.7 2,914,567 - -0.3 2,914,611 - +0.2 2,914,742 + BT2332 0.32 -1.2 2,914,743 - BT2332 0.32 -1.3 2,914,745 + BT2332 0.32 +0.4 2,914,746 - BT2332 0.33 -1.2 2,914,746 - BT2332 0.33 +0.5 2,914,759 + BT2332 0.36 -1.7 2,914,834 + BT2332 0.58 -0.7 2,914,834 + BT2332 0.58 +3.0 2,914,835 - BT2332 0.58 -1.7 2,914,872 - BT2332 0.68 -0.7 2,914,872 - BT2332 0.68 -0.7 2,914,941 + BT2332 0.88 +0.1 2,914,942 - BT2332 0.88 -2.7 2,914,978 + -1.2 2,914,983 + -0.7 2,914,983 + -1.5 2,914,983 + -0.9 2,915,015 + -1.3 2,915,138 + +0.3 2,915,142 + -1.2 2,915,280 + BT2333 0.14 +0.1 2,915,280 + BT2333 0.14 -0.3 2,915,280 + BT2333 0.14 -0.6 2,915,440 + BT2333 0.22 -2.0 2,915,462 + BT2333 0.23 +0.7 2,915,582 + BT2333 0.29 -1.3 2,915,588 + BT2333 0.29 -0.3 2,915,651 + BT2333 0.32 +0.7 2,915,717 + BT2333 0.35 +0.1 2,915,725 + BT2333 0.36 -1.7 2,915,852 + BT2333 0.42 -0.3 2,915,886 + BT2333 0.43 -2.4 2,915,886 + BT2333 0.43 -1.3 2,916,074 + BT2333 0.53 -1.8 2,916,197 + BT2333 0.59 -2.5 2,916,198 - BT2333 0.59 -0.9 2,916,210 + BT2333 0.59 -0.3 2,916,383 + BT2333 0.68 -0.7 2,916,447 + BT2333 0.71 -0.6 2,916,447 + BT2333 0.71 -0.9 2,916,462 + BT2333 0.72 -0.7 2,916,465 - BT2333 0.72 -1.7 2,916,639 + BT2333 0.80 +0.7 2,916,641 + BT2333 0.80 -0.5 2,916,719 + BT2333 0.84 -0.5 2,916,719 + BT2333 0.84 -1.3 2,916,720 - BT2333 0.84 -2.7 2,916,848 + -1.4 2,916,849 - +1.7 2,916,902 + -1.6 2,916,930 + -0.9 2,917,024 - -1.6 2,917,105 + BT2334 0.23 -1.3 2,917,105 + BT2334 0.23 -2.6 2,917,147 + BT2334 0.35 +0.3 2,917,147 + BT2334 0.35 +0.5 2,917,207 + BT2334 0.53 -0.2 2,917,207 + BT2334 0.53 -1.7 2,917,207 + BT2334 0.53 -3.0 2,917,207 + BT2334 0.53 -0.7 2,917,207 - BT2334 0.53 +1.2 2,917,213 + BT2334 0.54 -1.2 2,917,213 + BT2334 0.54 -1.2 2,917,214 - BT2334 0.55 -1.5 2,917,295 + BT2334 0.78 -0.5 2,917,338 + -1.8 2,917,361 - -2.8 2,917,369 + -1.4 2,917,369 + -0.5 2,917,411 + -0.6 2,917,411 + -0.5 2,917,431 - +0.3 2,917,433 + -1.5 2,917,434 - -1.9 2,917,435 + -1.9 2,917,435 + -2.7 2,917,435 + -1.4 2,917,435 + +2.7 2,917,435 + +0.1 2,917,436 - +0.1 2,917,444 + +0.3 2,917,444 + -0.9 2,917,445 - -2.6 2,917,574 + +0.1 2,917,574 + -0.1 2,917,574 + -0.3 2,917,574 + -1.3 2,917,574 + -3.6 2,917,575 - -2.0 2,917,575 - -1.6 2,917,575 - -0.7 2,917,576 + -0.3 2,917,623 + -0.5 2,917,720 - -0.5 2,917,720 - -0.7 2,917,729 + +0.1 2,917,732 + +1.1 2,917,733 - -1.3 2,917,746 - -1.8 2,917,746 - -0.2 2,917,774 + +0.1 2,917,775 - +0.4 2,917,775 - -0.9 2,917,785 + -2.5 2,917,917 + +0.1 2,917,996 - +0.7 2,918,013 + -0.8
Or see this region's nucleotide sequence