Experiment: Supernatant; Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (5 mM 3-keto-trehalose) (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20328 and SM_b20329 are separated by 165 nucleotides SM_b20329 and SM_b20330 overlap by 4 nucleotides SM_b20330 and SM_b20331 are separated by 212 nucleotides
SM_b20328: SM_b20328 - ABC transporter for D-trehalose/D-maltose/sucrose, ATPase component (ThuK) (from data) , at 331,283 to 332,311
_b20328
SM_b20329: SM_b20329 - required for glucoside 3-dehydrogenase activity on raffinose or trehalose (thuA) (EC 1.1.99.13) (from data) , at 332,477 to 333,289
_b20329
SM_b20330: SM_b20330 - glucoside 3-dehydrogenase; active on raffinose or trehalose (thuB) (EC 1.1.99.13) (from data) , at 333,286 to 334,383
_b20330
SM_b20331: SM_b20331 - hypothetical protein, at 334,596 to 334,952
_b20331
Position (kb)
333
334
335 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 332.395 kb on + strand at 332.396 kb on - strand at 332.751 kb on + strand, within SM_b20329 at 332.751 kb on + strand, within SM_b20329 at 332.752 kb on - strand, within SM_b20329 at 332.790 kb on + strand, within SM_b20329 at 332.790 kb on + strand, within SM_b20329 at 332.963 kb on + strand, within SM_b20329 at 332.963 kb on + strand, within SM_b20329 at 332.963 kb on + strand, within SM_b20329 at 332.963 kb on + strand, within SM_b20329 at 332.964 kb on - strand, within SM_b20329 at 332.964 kb on - strand, within SM_b20329 at 332.964 kb on - strand, within SM_b20329 at 333.073 kb on + strand, within SM_b20329 at 333.073 kb on + strand, within SM_b20329 at 333.081 kb on - strand, within SM_b20329 at 333.130 kb on - strand, within SM_b20329 at 333.130 kb on - strand, within SM_b20329 at 333.164 kb on + strand, within SM_b20329 at 333.165 kb on - strand, within SM_b20329 at 333.166 kb on + strand, within SM_b20329 at 333.166 kb on + strand at 333.182 kb on + strand, within SM_b20329 at 333.182 kb on + strand, within SM_b20329 at 333.183 kb on - strand, within SM_b20329 at 333.185 kb on - strand, within SM_b20329 at 333.185 kb on - strand, within SM_b20329 at 333.185 kb on - strand, within SM_b20329 at 333.748 kb on + strand, within SM_b20330 at 333.748 kb on + strand, within SM_b20330 at 333.749 kb on - strand, within SM_b20330 at 333.749 kb on - strand, within SM_b20330 at 333.967 kb on + strand at 333.968 kb on - strand, within SM_b20330 at 334.119 kb on + strand, within SM_b20330 at 334.119 kb on + strand, within SM_b20330 at 334.213 kb on + strand, within SM_b20330 at 334.214 kb on - strand, within SM_b20330 at 334.214 kb on - strand, within SM_b20330 at 334.391 kb on - strand at 334.466 kb on - strand at 334.495 kb on + strand at 334.574 kb on - strand at 334.598 kb on - strand at 334.598 kb on - strand at 334.618 kb on + strand at 335.004 kb on + strand at 335.004 kb on + strand at 335.004 kb on + strand at 335.043 kb on + strand at 335.136 kb on + strand at 335.147 kb on + strand at 335.148 kb on - strand at 335.148 kb on - strand at 335.154 kb on + strand at 335.154 kb on + strand at 335.154 kb on + strand at 335.154 kb on + strand at 335.155 kb on - strand at 335.155 kb on - strand at 335.155 kb on - strand at 335.158 kb on + strand at 335.159 kb on - strand at 335.159 kb on - strand at 335.236 kb on + strand at 335.327 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Agrobacterium rhizogenes K599 grown in Agro_defined_trehalose (5 mM 3-keto-trehalose) (C) remove 332,395 + -0.7 332,396 - +0.6 332,751 + SM_b20329 0.34 +0.3 332,751 + SM_b20329 0.34 -0.2 332,752 - SM_b20329 0.34 -0.8 332,790 + SM_b20329 0.38 -1.2 332,790 + SM_b20329 0.38 -0.9 332,963 + SM_b20329 0.60 +1.3 332,963 + SM_b20329 0.60 +0.7 332,963 + SM_b20329 0.60 -1.0 332,963 + SM_b20329 0.60 +0.4 332,964 - SM_b20329 0.60 +0.2 332,964 - SM_b20329 0.60 -0.5 332,964 - SM_b20329 0.60 +0.4 333,073 + SM_b20329 0.73 -0.6 333,073 + SM_b20329 0.73 -0.9 333,081 - SM_b20329 0.74 -1.5 333,130 - SM_b20329 0.80 -1.1 333,130 - SM_b20329 0.80 -0.8 333,164 + SM_b20329 0.85 -1.2 333,165 - SM_b20329 0.85 -0.8 333,166 + SM_b20329 0.85 -1.9 333,166 + -1.3 333,182 + SM_b20329 0.87 -0.7 333,182 + SM_b20329 0.87 -0.7 333,183 - SM_b20329 0.87 -1.3 333,185 - SM_b20329 0.87 -0.7 333,185 - SM_b20329 0.87 -0.3 333,185 - SM_b20329 0.87 -0.4 333,748 + SM_b20330 0.42 -0.3 333,748 + SM_b20330 0.42 -0.7 333,749 - SM_b20330 0.42 +0.1 333,749 - SM_b20330 0.42 -1.3 333,967 + +0.3 333,968 - SM_b20330 0.62 -0.8 334,119 + SM_b20330 0.76 -2.5 334,119 + SM_b20330 0.76 -0.8 334,213 + SM_b20330 0.84 -0.5 334,214 - SM_b20330 0.85 -0.4 334,214 - SM_b20330 0.85 -3.2 334,391 - -0.5 334,466 - +1.3 334,495 + -1.2 334,574 - -0.2 334,598 - +0.1 334,598 - -1.3 334,618 + -0.2 335,004 + +0.8 335,004 + +0.1 335,004 + -0.1 335,043 + +1.0 335,136 + +0.4 335,147 + -0.2 335,148 - +0.3 335,148 - +2.0 335,154 + -1.7 335,154 + +0.0 335,154 + +0.4 335,154 + -0.2 335,155 - -0.2 335,155 - -0.3 335,155 - -0.3 335,158 + +0.6 335,159 - +0.1 335,159 - +1.0 335,236 + +0.3 335,327 - +0.7
Or see this region's nucleotide sequence