Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0995 and Psest_0996 overlap by 4 nucleotides Psest_0996 and Psest_0997 are separated by 123 nucleotides Psest_0997 and Psest_0998 are separated by 1 nucleotides
Psest_0995: Psest_0995 - pantoate--beta-alanine ligase, at 1,078,150 to 1,079,010
_0995
Psest_0996: Psest_0996 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,079,007 to 1,079,807
_0996
Psest_0997: Psest_0997 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase, at 1,079,931 to 1,080,458
_0997
Psest_0998: Psest_0998 - poly(A) polymerase, at 1,080,460 to 1,081,854
_0998
Position (kb)
1079
1080
1081 Strain fitness (log2 ratio)
-1
0
1
2 at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.943 kb on + strand at 1079.944 kb on - strand at 1079.944 kb on - strand at 1081.359 kb on - strand, within Psest_0998 at 1081.362 kb on - strand, within Psest_0998
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,079,895 - +1.9 1,079,895 - +0.1 1,079,895 - +0.7 1,079,895 - +0.7 1,079,895 - +0.4 1,079,915 - +0.1 1,079,915 - +0.7 1,079,915 - +0.8 1,079,915 - +0.8 1,079,915 - +0.7 1,079,915 - -0.5 1,079,915 - -0.5 1,079,943 + -0.1 1,079,944 - +0.0 1,079,944 - -1.6 1,081,359 - Psest_0998 0.64 -1.3 1,081,362 - Psest_0998 0.65 +0.7
Or see this region's nucleotide sequence