Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3203 and Psest_3204 are separated by 66 nucleotides Psest_3204 and Psest_3205 are separated by 18 nucleotides Psest_3205 and Psest_3206 are separated by 76 nucleotides Psest_3206 and Psest_3207 overlap by 4 nucleotides Psest_3207 and Psest_3208 are separated by 221 nucleotides
Psest_3203: Psest_3203 - hypothetical protein, at 3,374,062 to 3,374,688
_3203
Psest_3204: Psest_3204 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 3,374,755 to 3,375,459
_3204
Psest_3205: Psest_3205 - FolB domain, at 3,375,478 to 3,375,852
_3205
Psest_3206: Psest_3206 - Uncharacterized protein conserved in bacteria, at 3,375,929 to 3,376,222
_3206
Psest_3207: Psest_3207 - HopJ type III effector protein., at 3,376,219 to 3,376,557
_3207
Psest_3208: Psest_3208 - Uncharacterized protein involved in cysteine biosynthesis, at 3,376,779 to 3,377,534
_3208
Position (kb)
3375
3376 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3374.573 kb on - strand, within Psest_3203 at 3374.686 kb on + strand at 3374.686 kb on + strand at 3374.686 kb on + strand at 3374.686 kb on + strand at 3374.687 kb on - strand at 3374.703 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.752 kb on + strand at 3374.753 kb on - strand at 3374.753 kb on - strand at 3374.753 kb on - strand at 3374.753 kb on - strand at 3374.753 kb on - strand at 3374.753 kb on - strand at 3374.753 kb on - strand at 3374.753 kb on - strand at 3374.753 kb on - strand at 3374.753 kb on - strand at 3374.753 kb on - strand at 3374.753 kb on - strand at 3374.854 kb on + strand, within Psest_3204 at 3374.854 kb on + strand, within Psest_3204 at 3374.937 kb on + strand, within Psest_3204 at 3375.069 kb on + strand, within Psest_3204 at 3375.070 kb on - strand, within Psest_3204 at 3375.070 kb on - strand, within Psest_3204 at 3375.070 kb on - strand, within Psest_3204 at 3375.070 kb on - strand, within Psest_3204 at 3375.070 kb on - strand, within Psest_3204 at 3375.070 kb on - strand, within Psest_3204 at 3375.070 kb on - strand, within Psest_3204 at 3375.183 kb on + strand, within Psest_3204 at 3375.183 kb on + strand, within Psest_3204 at 3375.183 kb on + strand, within Psest_3204 at 3375.183 kb on + strand, within Psest_3204 at 3375.377 kb on - strand, within Psest_3204 at 3375.377 kb on - strand, within Psest_3204 at 3375.377 kb on - strand, within Psest_3204 at 3375.409 kb on + strand at 3375.410 kb on - strand at 3375.410 kb on - strand at 3375.499 kb on - strand at 3375.538 kb on + strand, within Psest_3205 at 3375.538 kb on + strand, within Psest_3205 at 3375.538 kb on + strand, within Psest_3205 at 3375.538 kb on + strand, within Psest_3205 at 3375.538 kb on + strand, within Psest_3205 at 3375.538 kb on + strand, within Psest_3205 at 3375.538 kb on + strand, within Psest_3205 at 3375.538 kb on + strand, within Psest_3205 at 3375.538 kb on + strand, within Psest_3205 at 3375.538 kb on + strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.539 kb on - strand, within Psest_3205 at 3375.540 kb on + strand, within Psest_3205 at 3375.605 kb on - strand, within Psest_3205 at 3375.765 kb on - strand, within Psest_3205 at 3375.889 kb on + strand at 3376.068 kb on - strand, within Psest_3206 at 3376.068 kb on - strand, within Psest_3206 at 3376.068 kb on - strand, within Psest_3206 at 3376.068 kb on - strand, within Psest_3206 at 3376.068 kb on - strand, within Psest_3206 at 3376.068 kb on - strand, within Psest_3206 at 3376.130 kb on + strand, within Psest_3206 at 3376.130 kb on + strand, within Psest_3206 at 3376.130 kb on + strand, within Psest_3206 at 3376.143 kb on - strand, within Psest_3206 at 3376.303 kb on + strand, within Psest_3207 at 3376.304 kb on - strand, within Psest_3207 at 3376.304 kb on - strand, within Psest_3207 at 3376.304 kb on - strand, within Psest_3207 at 3376.304 kb on - strand, within Psest_3207 at 3376.304 kb on - strand, within Psest_3207 at 3376.304 kb on - strand, within Psest_3207 at 3376.304 kb on - strand, within Psest_3207 at 3376.304 kb on - strand, within Psest_3207 at 3376.304 kb on - strand, within Psest_3207 at 3376.304 kb on - strand, within Psest_3207 at 3376.309 kb on + strand, within Psest_3207 at 3376.310 kb on - strand, within Psest_3207 at 3376.310 kb on - strand, within Psest_3207 at 3376.555 kb on + strand at 3376.555 kb on + strand at 3376.555 kb on + strand at 3376.555 kb on + strand at 3376.563 kb on - strand at 3376.563 kb on - strand at 3376.600 kb on + strand at 3376.601 kb on - strand at 3376.671 kb on + strand at 3376.671 kb on + strand at 3376.671 kb on + strand at 3376.672 kb on - strand at 3376.672 kb on - strand at 3376.710 kb on + strand at 3376.710 kb on + strand at 3376.710 kb on + strand at 3376.710 kb on + strand at 3376.710 kb on + strand at 3376.710 kb on + strand at 3376.710 kb on + strand at 3376.711 kb on - strand at 3376.711 kb on - strand at 3376.711 kb on - strand at 3376.711 kb on - strand at 3376.711 kb on - strand at 3376.711 kb on - strand at 3376.711 kb on - strand at 3376.711 kb on - strand at 3376.711 kb on - strand at 3376.711 kb on - strand at 3376.719 kb on + strand at 3376.720 kb on - strand at 3376.720 kb on - strand at 3376.766 kb on - strand at 3376.809 kb on + strand at 3376.810 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 3,374,573 - Psest_3203 0.81 -1.4 3,374,686 + +0.2 3,374,686 + -0.8 3,374,686 + +2.4 3,374,686 + -0.6 3,374,687 - -0.5 3,374,703 + -0.1 3,374,752 + -2.3 3,374,752 + +0.6 3,374,752 + -1.4 3,374,752 + -1.1 3,374,752 + +1.6 3,374,752 + +0.4 3,374,752 + +2.3 3,374,752 + -1.3 3,374,752 + -0.8 3,374,752 + +0.6 3,374,752 + -1.1 3,374,752 + +0.8 3,374,752 + -0.5 3,374,752 + +0.9 3,374,752 + -0.7 3,374,752 + -2.7 3,374,752 + +0.6 3,374,752 + -1.0 3,374,752 + +0.8 3,374,752 + -0.3 3,374,752 + -0.3 3,374,753 - +4.1 3,374,753 - +2.8 3,374,753 - +2.9 3,374,753 - +0.7 3,374,753 - +1.8 3,374,753 - +2.4 3,374,753 - +1.2 3,374,753 - +0.8 3,374,753 - +0.2 3,374,753 - +1.8 3,374,753 - +0.7 3,374,753 - +3.3 3,374,854 + Psest_3204 0.14 -0.4 3,374,854 + Psest_3204 0.14 +0.5 3,374,937 + Psest_3204 0.26 +1.5 3,375,069 + Psest_3204 0.45 -0.3 3,375,070 - Psest_3204 0.45 +0.6 3,375,070 - Psest_3204 0.45 +1.0 3,375,070 - Psest_3204 0.45 +3.7 3,375,070 - Psest_3204 0.45 +0.7 3,375,070 - Psest_3204 0.45 +0.4 3,375,070 - Psest_3204 0.45 +2.0 3,375,070 - Psest_3204 0.45 +0.5 3,375,183 + Psest_3204 0.61 +0.3 3,375,183 + Psest_3204 0.61 +0.3 3,375,183 + Psest_3204 0.61 +2.2 3,375,183 + Psest_3204 0.61 +3.0 3,375,377 - Psest_3204 0.88 +0.6 3,375,377 - Psest_3204 0.88 -0.1 3,375,377 - Psest_3204 0.88 +2.1 3,375,409 + -1.0 3,375,410 - +1.2 3,375,410 - +2.7 3,375,499 - +4.6 3,375,538 + Psest_3205 0.16 +2.5 3,375,538 + Psest_3205 0.16 +1.3 3,375,538 + Psest_3205 0.16 +0.0 3,375,538 + Psest_3205 0.16 +1.1 3,375,538 + Psest_3205 0.16 +2.6 3,375,538 + Psest_3205 0.16 +2.7 3,375,538 + Psest_3205 0.16 +2.6 3,375,538 + Psest_3205 0.16 +3.8 3,375,538 + Psest_3205 0.16 +1.4 3,375,538 + Psest_3205 0.16 -0.5 3,375,539 - Psest_3205 0.16 +1.6 3,375,539 - Psest_3205 0.16 +0.3 3,375,539 - Psest_3205 0.16 -0.7 3,375,539 - Psest_3205 0.16 +0.6 3,375,539 - Psest_3205 0.16 +0.5 3,375,539 - Psest_3205 0.16 +1.0 3,375,539 - Psest_3205 0.16 +2.3 3,375,539 - Psest_3205 0.16 -1.0 3,375,539 - Psest_3205 0.16 +0.8 3,375,539 - Psest_3205 0.16 -0.1 3,375,539 - Psest_3205 0.16 +0.0 3,375,539 - Psest_3205 0.16 +2.2 3,375,539 - Psest_3205 0.16 +1.9 3,375,539 - Psest_3205 0.16 +2.8 3,375,539 - Psest_3205 0.16 +3.1 3,375,540 + Psest_3205 0.17 +1.0 3,375,605 - Psest_3205 0.34 +1.2 3,375,765 - Psest_3205 0.77 -0.5 3,375,889 + -0.3 3,376,068 - Psest_3206 0.47 +0.8 3,376,068 - Psest_3206 0.47 +1.0 3,376,068 - Psest_3206 0.47 +0.7 3,376,068 - Psest_3206 0.47 +0.5 3,376,068 - Psest_3206 0.47 +0.3 3,376,068 - Psest_3206 0.47 +0.0 3,376,130 + Psest_3206 0.68 -0.7 3,376,130 + Psest_3206 0.68 +0.7 3,376,130 + Psest_3206 0.68 +1.5 3,376,143 - Psest_3206 0.73 -0.4 3,376,303 + Psest_3207 0.25 -1.2 3,376,304 - Psest_3207 0.25 +0.3 3,376,304 - Psest_3207 0.25 -0.8 3,376,304 - Psest_3207 0.25 -0.4 3,376,304 - Psest_3207 0.25 -0.9 3,376,304 - Psest_3207 0.25 -1.5 3,376,304 - Psest_3207 0.25 +1.8 3,376,304 - Psest_3207 0.25 -1.2 3,376,304 - Psest_3207 0.25 +0.8 3,376,304 - Psest_3207 0.25 -0.3 3,376,304 - Psest_3207 0.25 -0.4 3,376,309 + Psest_3207 0.27 -0.2 3,376,310 - Psest_3207 0.27 -0.4 3,376,310 - Psest_3207 0.27 +0.8 3,376,555 + -0.2 3,376,555 + +1.6 3,376,555 + -2.1 3,376,555 + +0.1 3,376,563 - +0.1 3,376,563 - -1.4 3,376,600 + +0.1 3,376,601 - -0.6 3,376,671 + -1.6 3,376,671 + -2.3 3,376,671 + -1.2 3,376,672 - +1.2 3,376,672 - -1.3 3,376,710 + -0.9 3,376,710 + -0.3 3,376,710 + +1.6 3,376,710 + -0.7 3,376,710 + -0.9 3,376,710 + +1.2 3,376,710 + +0.0 3,376,711 - +0.1 3,376,711 - +0.3 3,376,711 - +0.0 3,376,711 - +0.3 3,376,711 - -0.5 3,376,711 - +1.1 3,376,711 - +0.3 3,376,711 - +0.7 3,376,711 - +0.0 3,376,711 - -0.2 3,376,719 + +1.5 3,376,720 - +0.3 3,376,720 - -1.2 3,376,766 - -0.2 3,376,809 + -2.2 3,376,810 - -0.7
Or see this region's nucleotide sequence