Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1549

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1548 and Psest_1549 are separated by 64 nucleotidesPsest_1549 and Psest_1550 are separated by 95 nucleotides Psest_1548: Psest_1548 - peptide chain release factor 2, at 1,648,582 to 1,649,677 _1548 Psest_1549: Psest_1549 - Lysyl-tRNA synthetase (class II), at 1,649,742 to 1,651,244 _1549 Psest_1550: Psest_1550 - conserved hypothetical protein, at 1,651,340 to 1,652,635 _1550 Position (kb) 1649 1650 1651 1652Strain fitness (log2 ratio) -1 0 1 2at 1651.335 kb on + strandat 1651.335 kb on + strandat 1651.335 kb on + strandat 1651.335 kb on + strandat 1651.336 kb on - strandat 1651.368 kb on - strandat 1651.515 kb on - strand, within Psest_1550at 1651.517 kb on + strand, within Psest_1550at 1651.518 kb on - strand, within Psest_1550at 1651.649 kb on + strand, within Psest_1550at 1651.650 kb on - strand, within Psest_1550at 1651.791 kb on - strand, within Psest_1550at 1651.967 kb on + strand, within Psest_1550at 1651.968 kb on - strand, within Psest_1550

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,651,335 + -0.8
1,651,335 + -1.1
1,651,335 + -0.8
1,651,335 + -0.4
1,651,336 - -0.6
1,651,368 - +0.1
1,651,515 - Psest_1550 0.14 +0.5
1,651,517 + Psest_1550 0.14 +2.3
1,651,518 - Psest_1550 0.14 +1.0
1,651,649 + Psest_1550 0.24 -0.4
1,651,650 - Psest_1550 0.24 -0.8
1,651,791 - Psest_1550 0.35 +0.3
1,651,967 + Psest_1550 0.48 -0.7
1,651,968 - Psest_1550 0.48 +0.3

Or see this region's nucleotide sequence