Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1054 and Psest_1055 are separated by 71 nucleotides Psest_1055 and Psest_1056 are separated by 348 nucleotides
Psest_1054: Psest_1054 - Diaminopimelate decarboxylase, at 1,142,571 to 1,143,668
_1054
Psest_1055: Psest_1055 - Saccharopine dehydrogenase and related proteins, at 1,143,740 to 1,144,936
_1055
Psest_1056: Psest_1056 - 2-isopropylmalate synthase, bacterial type, at 1,145,285 to 1,146,829
_1056
Position (kb)
1143
1144
1145 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1142.956 kb on - strand, within Psest_1054 at 1143.245 kb on - strand, within Psest_1054 at 1143.322 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.433 kb on - strand, within Psest_1054 at 1143.481 kb on - strand, within Psest_1054 at 1143.649 kb on - strand at 1143.649 kb on - strand at 1143.677 kb on + strand at 1143.678 kb on - strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.712 kb on + strand at 1143.713 kb on - strand at 1143.713 kb on - strand at 1143.751 kb on + strand at 1143.751 kb on + strand at 1143.751 kb on + strand at 1143.751 kb on + strand at 1143.752 kb on - strand at 1143.752 kb on - strand at 1143.790 kb on + strand at 1143.881 kb on + strand, within Psest_1055 at 1143.882 kb on - strand, within Psest_1055 at 1143.882 kb on - strand, within Psest_1055 at 1143.976 kb on + strand, within Psest_1055 at 1143.976 kb on + strand, within Psest_1055 at 1143.977 kb on - strand, within Psest_1055 at 1143.977 kb on - strand, within Psest_1055 at 1143.977 kb on - strand, within Psest_1055 at 1143.977 kb on - strand, within Psest_1055 at 1144.074 kb on - strand, within Psest_1055 at 1144.082 kb on + strand, within Psest_1055 at 1144.082 kb on - strand, within Psest_1055 at 1144.083 kb on - strand, within Psest_1055 at 1144.083 kb on - strand, within Psest_1055 at 1144.194 kb on - strand, within Psest_1055 at 1144.194 kb on - strand, within Psest_1055 at 1144.194 kb on - strand, within Psest_1055 at 1144.194 kb on - strand, within Psest_1055 at 1144.253 kb on + strand, within Psest_1055 at 1144.253 kb on + strand, within Psest_1055 at 1144.254 kb on - strand, within Psest_1055 at 1144.254 kb on - strand, within Psest_1055 at 1144.254 kb on - strand, within Psest_1055 at 1144.254 kb on - strand, within Psest_1055 at 1144.310 kb on + strand, within Psest_1055 at 1144.310 kb on + strand, within Psest_1055 at 1144.400 kb on + strand, within Psest_1055 at 1144.400 kb on + strand, within Psest_1055 at 1144.450 kb on - strand, within Psest_1055 at 1144.450 kb on - strand, within Psest_1055 at 1144.487 kb on + strand, within Psest_1055 at 1144.487 kb on + strand, within Psest_1055 at 1144.487 kb on + strand, within Psest_1055 at 1144.487 kb on + strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.488 kb on - strand, within Psest_1055 at 1144.501 kb on + strand, within Psest_1055 at 1144.502 kb on - strand, within Psest_1055 at 1144.625 kb on + strand, within Psest_1055 at 1144.626 kb on - strand, within Psest_1055 at 1144.697 kb on + strand, within Psest_1055 at 1144.697 kb on + strand, within Psest_1055 at 1144.698 kb on - strand, within Psest_1055 at 1144.698 kb on - strand, within Psest_1055 at 1144.698 kb on - strand, within Psest_1055 at 1144.724 kb on + strand, within Psest_1055 at 1144.754 kb on + strand, within Psest_1055 at 1144.754 kb on + strand, within Psest_1055 at 1144.755 kb on - strand, within Psest_1055 at 1144.767 kb on + strand, within Psest_1055 at 1144.988 kb on + strand at 1144.996 kb on + strand at 1144.997 kb on - strand at 1144.997 kb on - strand at 1144.997 kb on - strand at 1144.997 kb on - strand at 1145.036 kb on + strand at 1145.037 kb on - strand at 1145.241 kb on + strand at 1145.242 kb on - strand at 1145.284 kb on + strand at 1145.615 kb on + strand, within Psest_1056 at 1145.615 kb on + strand, within Psest_1056 at 1145.828 kb on + strand, within Psest_1056
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,142,956 - Psest_1054 0.35 -2.9 1,143,245 - Psest_1054 0.61 -0.4 1,143,322 - Psest_1054 0.68 -1.4 1,143,433 - Psest_1054 0.79 -1.2 1,143,433 - Psest_1054 0.79 +0.8 1,143,433 - Psest_1054 0.79 -2.2 1,143,433 - Psest_1054 0.79 -2.2 1,143,433 - Psest_1054 0.79 -0.0 1,143,433 - Psest_1054 0.79 -1.7 1,143,433 - Psest_1054 0.79 -2.8 1,143,433 - Psest_1054 0.79 -0.3 1,143,481 - Psest_1054 0.83 -1.3 1,143,649 - +0.2 1,143,649 - -1.4 1,143,677 + -0.2 1,143,678 - -1.4 1,143,712 + -1.2 1,143,712 + -1.8 1,143,712 + -0.4 1,143,712 + -1.5 1,143,712 + +0.2 1,143,712 + -0.7 1,143,712 + -0.6 1,143,713 - -0.7 1,143,713 - -0.2 1,143,751 + -2.2 1,143,751 + -0.6 1,143,751 + +0.9 1,143,751 + -1.5 1,143,752 - -1.2 1,143,752 - -0.9 1,143,790 + -2.3 1,143,881 + Psest_1055 0.12 -2.7 1,143,882 - Psest_1055 0.12 -0.4 1,143,882 - Psest_1055 0.12 -1.7 1,143,976 + Psest_1055 0.20 -1.6 1,143,976 + Psest_1055 0.20 -1.7 1,143,977 - Psest_1055 0.20 -1.3 1,143,977 - Psest_1055 0.20 -1.8 1,143,977 - Psest_1055 0.20 +0.3 1,143,977 - Psest_1055 0.20 -2.6 1,144,074 - Psest_1055 0.28 +0.8 1,144,082 + Psest_1055 0.29 +1.8 1,144,082 - Psest_1055 0.29 -0.7 1,144,083 - Psest_1055 0.29 -1.3 1,144,083 - Psest_1055 0.29 -1.4 1,144,194 - Psest_1055 0.38 -0.8 1,144,194 - Psest_1055 0.38 -1.6 1,144,194 - Psest_1055 0.38 -0.6 1,144,194 - Psest_1055 0.38 -1.2 1,144,253 + Psest_1055 0.43 -0.9 1,144,253 + Psest_1055 0.43 -3.2 1,144,254 - Psest_1055 0.43 -1.9 1,144,254 - Psest_1055 0.43 -1.7 1,144,254 - Psest_1055 0.43 -2.7 1,144,254 - Psest_1055 0.43 +0.5 1,144,310 + Psest_1055 0.48 -0.4 1,144,310 + Psest_1055 0.48 -0.3 1,144,400 + Psest_1055 0.55 -1.8 1,144,400 + Psest_1055 0.55 +1.0 1,144,450 - Psest_1055 0.59 -0.5 1,144,450 - Psest_1055 0.59 -0.1 1,144,487 + Psest_1055 0.62 -1.8 1,144,487 + Psest_1055 0.62 -1.6 1,144,487 + Psest_1055 0.62 +0.4 1,144,487 + Psest_1055 0.62 -0.1 1,144,488 - Psest_1055 0.62 -0.4 1,144,488 - Psest_1055 0.62 +1.9 1,144,488 - Psest_1055 0.62 -1.8 1,144,488 - Psest_1055 0.62 -0.7 1,144,488 - Psest_1055 0.62 -1.5 1,144,488 - Psest_1055 0.62 -1.8 1,144,488 - Psest_1055 0.62 -3.6 1,144,488 - Psest_1055 0.62 -0.7 1,144,501 + Psest_1055 0.64 -0.6 1,144,502 - Psest_1055 0.64 -0.4 1,144,625 + Psest_1055 0.74 -0.5 1,144,626 - Psest_1055 0.74 -1.8 1,144,697 + Psest_1055 0.80 -1.2 1,144,697 + Psest_1055 0.80 +0.5 1,144,698 - Psest_1055 0.80 -1.8 1,144,698 - Psest_1055 0.80 -2.7 1,144,698 - Psest_1055 0.80 -0.4 1,144,724 + Psest_1055 0.82 -0.2 1,144,754 + Psest_1055 0.85 -0.3 1,144,754 + Psest_1055 0.85 -1.5 1,144,755 - Psest_1055 0.85 -1.4 1,144,767 + Psest_1055 0.86 -2.2 1,144,988 + +0.1 1,144,996 + -1.4 1,144,997 - -0.8 1,144,997 - +0.6 1,144,997 - -0.3 1,144,997 - -1.0 1,145,036 + -0.3 1,145,037 - +0.4 1,145,241 + +1.5 1,145,242 - -0.0 1,145,284 + +3.2 1,145,615 + Psest_1056 0.21 +1.8 1,145,615 + Psest_1056 0.21 -0.1 1,145,828 + Psest_1056 0.35 -0.7
Or see this region's nucleotide sequence