Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0397

Experiment: Nickel (II) chloride 0.9 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0396 and Psest_0397 are separated by 90 nucleotidesPsest_0397 and Psest_0398 are separated by 115 nucleotides Psest_0396: Psest_0396 - Uncharacterized conserved protein, at 425,924 to 427,384 _0396 Psest_0397: Psest_0397 - Small-conductance mechanosensitive channel, at 427,475 to 430,765 _0397 Psest_0398: Psest_0398 - NhaP-type Na+/H+ and K+/H+ antiporters with a unique C-terminal domain, at 430,881 to 432,626 _0398 Position (kb) 427 428 429 430 431Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 426.525 kb on + strand, within Psest_0396at 426.588 kb on + strand, within Psest_0396at 426.588 kb on + strand, within Psest_0396at 426.589 kb on - strand, within Psest_0396at 426.589 kb on - strand, within Psest_0396at 426.589 kb on - strand, within Psest_0396at 426.810 kb on + strand, within Psest_0396at 426.811 kb on - strand, within Psest_0396at 426.811 kb on - strand, within Psest_0396at 426.811 kb on - strand, within Psest_0396at 426.813 kb on + strand, within Psest_0396at 426.813 kb on + strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 427.131 kb on + strand, within Psest_0396at 427.131 kb on + strand, within Psest_0396at 427.132 kb on - strand, within Psest_0396at 427.132 kb on - strand, within Psest_0396at 427.132 kb on - strand, within Psest_0396at 427.132 kb on - strand, within Psest_0396at 427.132 kb on - strand, within Psest_0396at 427.156 kb on - strand, within Psest_0396at 427.156 kb on - strand, within Psest_0396at 427.280 kb on + strandat 427.280 kb on + strandat 427.280 kb on + strandat 427.280 kb on + strandat 427.281 kb on - strandat 427.281 kb on - strandat 427.403 kb on + strandat 427.404 kb on - strandat 427.435 kb on + strandat 427.779 kb on + strandat 427.779 kb on + strandat 427.779 kb on + strandat 427.779 kb on + strandat 427.780 kb on - strandat 427.780 kb on - strandat 427.780 kb on - strandat 428.249 kb on + strand, within Psest_0397at 428.364 kb on + strand, within Psest_0397at 428.365 kb on - strand, within Psest_0397at 428.365 kb on - strand, within Psest_0397at 428.365 kb on - strand, within Psest_0397at 428.365 kb on - strand, within Psest_0397at 428.392 kb on - strand, within Psest_0397at 428.392 kb on - strand, within Psest_0397at 428.392 kb on - strand, within Psest_0397at 428.427 kb on + strand, within Psest_0397at 428.427 kb on + strand, within Psest_0397at 428.665 kb on + strand, within Psest_0397at 428.666 kb on - strand, within Psest_0397at 428.700 kb on + strand, within Psest_0397at 428.700 kb on + strand, within Psest_0397at 428.701 kb on - strand, within Psest_0397at 428.701 kb on - strand, within Psest_0397at 428.701 kb on - strand, within Psest_0397at 428.701 kb on - strand, within Psest_0397at 428.701 kb on - strand, within Psest_0397at 428.701 kb on - strand, within Psest_0397at 428.701 kb on - strand, within Psest_0397at 428.701 kb on - strand, within Psest_0397at 428.703 kb on + strand, within Psest_0397at 428.703 kb on + strand, within Psest_0397at 428.703 kb on + strand, within Psest_0397at 428.703 kb on + strand, within Psest_0397at 428.703 kb on + strand, within Psest_0397at 428.703 kb on + strand, within Psest_0397at 428.703 kb on + strand, within Psest_0397at 428.703 kb on + strand, within Psest_0397at 428.703 kb on + strand, within Psest_0397at 428.704 kb on - strand, within Psest_0397at 428.704 kb on - strand, within Psest_0397at 428.704 kb on - strand, within Psest_0397at 429.048 kb on + strand, within Psest_0397at 429.048 kb on + strand, within Psest_0397at 429.049 kb on - strand, within Psest_0397at 429.049 kb on - strand, within Psest_0397at 429.049 kb on - strand, within Psest_0397at 429.099 kb on + strand, within Psest_0397at 429.099 kb on + strand, within Psest_0397at 429.099 kb on + strand, within Psest_0397at 429.100 kb on - strand, within Psest_0397at 429.100 kb on - strand, within Psest_0397at 429.951 kb on + strand, within Psest_0397at 430.471 kb on - strandat 430.690 kb on + strandat 430.882 kb on + strandat 431.252 kb on + strand, within Psest_0398

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.9 mM
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426,525 + Psest_0396 0.41 -1.4
426,588 + Psest_0396 0.45 -0.3
426,588 + Psest_0396 0.45 -2.4
426,589 - Psest_0396 0.46 -0.2
426,589 - Psest_0396 0.46 +0.7
426,589 - Psest_0396 0.46 -1.6
426,810 + Psest_0396 0.61 +0.5
426,811 - Psest_0396 0.61 +0.4
426,811 - Psest_0396 0.61 +1.9
426,811 - Psest_0396 0.61 -0.3
426,813 + Psest_0396 0.61 -0.9
426,813 + Psest_0396 0.61 -1.7
426,814 - Psest_0396 0.61 +0.1
426,814 - Psest_0396 0.61 +0.0
426,814 - Psest_0396 0.61 +0.7
426,814 - Psest_0396 0.61 +1.7
426,814 - Psest_0396 0.61 +1.8
426,814 - Psest_0396 0.61 +1.6
426,814 - Psest_0396 0.61 -0.2
427,131 + Psest_0396 0.83 +0.3
427,131 + Psest_0396 0.83 -0.9
427,132 - Psest_0396 0.83 -2.0
427,132 - Psest_0396 0.83 -1.9
427,132 - Psest_0396 0.83 -0.9
427,132 - Psest_0396 0.83 +0.7
427,132 - Psest_0396 0.83 +0.1
427,156 - Psest_0396 0.84 -0.0
427,156 - Psest_0396 0.84 -0.2
427,280 + -2.2
427,280 + -0.6
427,280 + -2.4
427,280 + +1.2
427,281 - -1.0
427,281 - +0.2
427,403 + -0.2
427,404 - -2.4
427,435 + -0.7
427,779 + -1.5
427,779 + -2.0
427,779 + -2.7
427,779 + -3.1
427,780 - -1.2
427,780 - -1.8
427,780 - -2.5
428,249 + Psest_0397 0.24 -1.6
428,364 + Psest_0397 0.27 -1.6
428,365 - Psest_0397 0.27 -1.4
428,365 - Psest_0397 0.27 -1.7
428,365 - Psest_0397 0.27 -3.8
428,365 - Psest_0397 0.27 -2.0
428,392 - Psest_0397 0.28 -3.5
428,392 - Psest_0397 0.28 -2.9
428,392 - Psest_0397 0.28 -2.8
428,427 + Psest_0397 0.29 -2.1
428,427 + Psest_0397 0.29 -3.7
428,665 + Psest_0397 0.36 -2.8
428,666 - Psest_0397 0.36 -3.3
428,700 + Psest_0397 0.37 -2.5
428,700 + Psest_0397 0.37 -2.5
428,701 - Psest_0397 0.37 -2.0
428,701 - Psest_0397 0.37 -4.4
428,701 - Psest_0397 0.37 -2.2
428,701 - Psest_0397 0.37 -2.9
428,701 - Psest_0397 0.37 -2.7
428,701 - Psest_0397 0.37 -2.9
428,701 - Psest_0397 0.37 -4.1
428,701 - Psest_0397 0.37 -3.3
428,703 + Psest_0397 0.37 -2.2
428,703 + Psest_0397 0.37 -1.4
428,703 + Psest_0397 0.37 -0.7
428,703 + Psest_0397 0.37 -4.1
428,703 + Psest_0397 0.37 -0.9
428,703 + Psest_0397 0.37 -3.0
428,703 + Psest_0397 0.37 -1.7
428,703 + Psest_0397 0.37 -2.0
428,703 + Psest_0397 0.37 -1.7
428,704 - Psest_0397 0.37 -2.0
428,704 - Psest_0397 0.37 -4.5
428,704 - Psest_0397 0.37 -0.5
429,048 + Psest_0397 0.48 -1.9
429,048 + Psest_0397 0.48 -1.6
429,049 - Psest_0397 0.48 -2.6
429,049 - Psest_0397 0.48 -2.7
429,049 - Psest_0397 0.48 -2.0
429,099 + Psest_0397 0.49 -3.2
429,099 + Psest_0397 0.49 +0.3
429,099 + Psest_0397 0.49 -1.4
429,100 - Psest_0397 0.49 -2.4
429,100 - Psest_0397 0.49 -3.3
429,951 + Psest_0397 0.75 -3.6
430,471 - -3.1
430,690 + -1.9
430,882 + -1.3
431,252 + Psest_0398 0.21 +0.3

Or see this region's nucleotide sequence