Experiment: Nickel (II) chloride 0.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0112 and Psest_0113 are separated by 58 nucleotides Psest_0113 and Psest_0114 overlap by 4 nucleotides Psest_0114 and Psest_0115 are separated by 191 nucleotides
Psest_0112: Psest_0112 - Transcriptional regulator, at 110,797 to 111,714
_0112
Psest_0113: Psest_0113 - Uncharacterized protein conserved in bacteria, at 111,773 to 112,414
_0113
Psest_0114: Psest_0114 - RNA polymerase sigma factor, sigma-70 family, at 112,411 to 112,980
_0114
Psest_0115: Psest_0115 - Predicted integral membrane protein, at 113,172 to 113,444
_0115
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 110.819 kb on + strand at 110.819 kb on + strand at 110.819 kb on + strand at 110.819 kb on + strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.820 kb on - strand at 110.949 kb on - strand, within Psest_0112 at 111.296 kb on + strand, within Psest_0112 at 111.297 kb on - strand, within Psest_0112 at 111.297 kb on - strand, within Psest_0112 at 111.297 kb on - strand, within Psest_0112 at 111.297 kb on - strand, within Psest_0112 at 111.401 kb on + strand, within Psest_0112 at 111.402 kb on - strand, within Psest_0112 at 111.402 kb on - strand, within Psest_0112 at 111.402 kb on - strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.512 kb on + strand, within Psest_0112 at 111.744 kb on + strand at 111.745 kb on - strand at 111.806 kb on - strand at 111.841 kb on - strand, within Psest_0113 at 112.281 kb on + strand, within Psest_0113 at 112.281 kb on + strand, within Psest_0113 at 112.577 kb on - strand, within Psest_0114 at 112.789 kb on + strand, within Psest_0114 at 112.789 kb on + strand, within Psest_0114 at 112.968 kb on + strand at 112.969 kb on - strand at 112.969 kb on - strand at 113.112 kb on + strand at 113.112 kb on + strand at 113.112 kb on + strand at 113.112 kb on + strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.384 kb on + strand, within Psest_0115
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.9 mM remove 110,819 + +0.9 110,819 + -1.1 110,819 + -0.2 110,819 + +0.2 110,820 - -1.5 110,820 - -0.7 110,820 - +1.7 110,820 - +0.3 110,820 - -1.2 110,820 - -1.1 110,820 - -0.5 110,949 - Psest_0112 0.17 -0.2 111,296 + Psest_0112 0.54 +0.3 111,297 - Psest_0112 0.54 +0.7 111,297 - Psest_0112 0.54 -0.2 111,297 - Psest_0112 0.54 +1.0 111,297 - Psest_0112 0.54 -0.4 111,401 + Psest_0112 0.66 +0.9 111,402 - Psest_0112 0.66 -2.0 111,402 - Psest_0112 0.66 -0.1 111,402 - Psest_0112 0.66 +0.3 111,512 + Psest_0112 0.78 +1.5 111,512 + Psest_0112 0.78 -1.8 111,512 + Psest_0112 0.78 -0.5 111,512 + Psest_0112 0.78 -1.3 111,512 + Psest_0112 0.78 +0.0 111,512 + Psest_0112 0.78 -0.6 111,744 + -1.1 111,745 - +0.9 111,806 - +2.1 111,841 - Psest_0113 0.11 -0.3 112,281 + Psest_0113 0.79 -0.7 112,281 + Psest_0113 0.79 -1.7 112,577 - Psest_0114 0.29 -0.0 112,789 + Psest_0114 0.66 +0.3 112,789 + Psest_0114 0.66 +0.5 112,968 + +0.7 112,969 - -0.0 112,969 - +1.0 113,112 + -1.1 113,112 + +1.7 113,112 + -1.3 113,112 + -0.2 113,113 - +0.4 113,113 - -0.0 113,113 - -1.1 113,113 - +0.1 113,113 - +1.0 113,384 + Psest_0115 0.78 -0.7
Or see this region's nucleotide sequence