Experiment: Nickel (II) chloride 0.75 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0013 and Psest_0014 are separated by 26 nucleotides Psest_0014 and Psest_0015 are separated by 25 nucleotides Psest_0015 and Psest_0016 are separated by 146 nucleotides Psest_0016 and Psest_0017 overlap by 4 nucleotides Psest_0017 and Psest_0018 overlap by 4 nucleotides
Psest_0013: Psest_0013 - Lauroyl/myristoyl acyltransferase, at 15,128 to 16,015
_0013
Psest_0014: Psest_0014 - PilZ domain., at 16,042 to 16,308
_0014
Psest_0015: Psest_0015 - Tetratricopeptide repeat., at 16,334 to 16,651
_0015
Psest_0016: Psest_0016 - ectoine/hydroxyectoine ABC transporter, ATP-binding protein, at 16,798 to 17,634
_0016
Psest_0017: Psest_0017 - ectoine/hydroxyectoine ABC transporter, permease protein EhuD, at 17,631 to 18,290
_0017
Psest_0018: Psest_0018 - ectoine/hydroxyectoine ABC transporter, permease protein EhuC, at 18,287 to 18,946
_0018
Position (kb) 16
17
18 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.932 kb on + strand at 15.932 kb on + strand at 15.932 kb on + strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.944 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 16.041 kb on + strand at 16.042 kb on - strand at 16.042 kb on - strand at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.157 kb on - strand, within Psest_0014 at 16.181 kb on + strand, within Psest_0014 at 16.181 kb on + strand, within Psest_0014 at 16.182 kb on - strand, within Psest_0014 at 16.506 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.576 kb on - strand, within Psest_0015 at 16.698 kb on + strand at 16.699 kb on - strand at 16.733 kb on + strand at 16.734 kb on - strand at 16.734 kb on - strand at 16.734 kb on - strand at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.288 kb on - strand, within Psest_0016 at 17.288 kb on - strand, within Psest_0016 at 17.288 kb on - strand, within Psest_0016 at 17.416 kb on + strand, within Psest_0016 at 17.417 kb on - strand, within Psest_0016 at 17.628 kb on + strand at 17.629 kb on - strand at 17.629 kb on - strand at 17.833 kb on + strand, within Psest_0017 at 17.833 kb on + strand, within Psest_0017 at 17.833 kb on + strand, within Psest_0017 at 17.834 kb on - strand, within Psest_0017 at 17.834 kb on - strand, within Psest_0017 at 17.834 kb on - strand, within Psest_0017 at 17.834 kb on - strand, within Psest_0017 at 17.983 kb on + strand, within Psest_0017 at 17.983 kb on + strand, within Psest_0017 at 17.983 kb on + strand, within Psest_0017 at 17.983 kb on + strand, within Psest_0017 at 17.984 kb on - strand, within Psest_0017 at 17.992 kb on + strand, within Psest_0017 at 18.046 kb on + strand, within Psest_0017 at 18.047 kb on - strand, within Psest_0017 at 18.057 kb on + strand, within Psest_0017 at 18.057 kb on + strand, within Psest_0017 at 18.058 kb on - strand, within Psest_0017 at 18.058 kb on - strand, within Psest_0017 at 18.058 kb on - strand, within Psest_0017 at 18.058 kb on - strand, within Psest_0017 at 18.061 kb on + strand, within Psest_0017 at 18.061 kb on + strand, within Psest_0017 at 18.061 kb on + strand, within Psest_0017 at 18.061 kb on + strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.062 kb on - strand, within Psest_0017 at 18.073 kb on + strand, within Psest_0017 at 18.073 kb on + strand, within Psest_0017 at 18.074 kb on - strand, within Psest_0017 at 18.074 kb on - strand, within Psest_0017 at 18.375 kb on + strand, within Psest_0018 at 18.375 kb on + strand, within Psest_0018 at 18.375 kb on + strand, within Psest_0018 at 18.375 kb on + strand, within Psest_0018 at 18.375 kb on + strand, within Psest_0018 at 18.375 kb on + strand, within Psest_0018 at 18.375 kb on + strand, within Psest_0018 at 18.375 kb on + strand, within Psest_0018 at 18.376 kb on - strand, within Psest_0018 at 18.376 kb on - strand, within Psest_0018 at 18.376 kb on - strand, within Psest_0018 at 18.376 kb on - strand, within Psest_0018 at 18.376 kb on - strand, within Psest_0018 at 18.376 kb on - strand, within Psest_0018
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.75 mM remove 15,878 + Psest_0013 0.84 +0.5 15,878 + Psest_0013 0.84 -0.5 15,878 + Psest_0013 0.84 +0.3 15,878 + Psest_0013 0.84 +0.4 15,878 + Psest_0013 0.84 +0.3 15,878 + Psest_0013 0.84 -0.1 15,878 + Psest_0013 0.84 -1.4 15,878 + Psest_0013 0.84 +0.2 15,878 + Psest_0013 0.84 -0.8 15,878 + Psest_0013 0.84 +1.2 15,878 + Psest_0013 0.84 -1.0 15,879 - Psest_0013 0.85 +0.0 15,879 - Psest_0013 0.85 +0.7 15,879 - Psest_0013 0.85 -0.3 15,879 - Psest_0013 0.85 -1.5 15,879 - Psest_0013 0.85 +0.3 15,879 - Psest_0013 0.85 -1.6 15,879 - Psest_0013 0.85 -0.3 15,879 - Psest_0013 0.85 -0.3 15,932 + +0.4 15,932 + -1.0 15,932 + -2.6 15,933 - -0.5 15,933 - -1.6 15,933 - -0.1 15,933 - -0.3 15,944 + +0.3 15,956 + +0.2 15,956 + +0.3 15,956 + +1.7 15,956 + -1.3 15,956 + -0.8 15,956 + +1.3 15,956 + -0.5 15,956 + +1.2 15,956 + -2.2 15,956 + +1.3 15,956 + +0.7 15,956 + +0.0 15,956 + +1.9 15,956 + -0.5 15,956 + +1.5 15,956 + +0.7 15,956 + +0.3 15,957 - +0.3 15,957 - +1.3 15,957 - +0.3 15,957 - +1.3 15,957 - +1.7 15,957 - +0.3 15,957 - +2.3 16,041 + -2.3 16,042 - -1.1 16,042 - -1.1 16,156 + Psest_0014 0.43 -0.8 16,156 + Psest_0014 0.43 -0.7 16,156 + Psest_0014 0.43 +0.2 16,156 + Psest_0014 0.43 +1.0 16,157 - Psest_0014 0.43 +0.4 16,181 + Psest_0014 0.52 +1.0 16,181 + Psest_0014 0.52 -1.3 16,182 - Psest_0014 0.52 -0.9 16,506 + Psest_0015 0.54 -0.8 16,575 + Psest_0015 0.76 +1.4 16,575 + Psest_0015 0.76 +0.1 16,575 + Psest_0015 0.76 +1.1 16,576 - Psest_0015 0.76 -1.0 16,698 + +0.1 16,699 - +0.3 16,733 + -0.4 16,734 - -0.0 16,734 - +0.9 16,734 - -0.4 17,287 + Psest_0016 0.58 +0.5 17,287 + Psest_0016 0.58 +1.9 17,287 + Psest_0016 0.58 -1.0 17,287 + Psest_0016 0.58 -0.9 17,287 + Psest_0016 0.58 -0.7 17,287 + Psest_0016 0.58 -1.1 17,287 + Psest_0016 0.58 -1.5 17,288 - Psest_0016 0.59 +1.0 17,288 - Psest_0016 0.59 +4.3 17,288 - Psest_0016 0.59 +0.5 17,416 + Psest_0016 0.74 -0.2 17,417 - Psest_0016 0.74 +2.2 17,628 + -0.2 17,629 - +0.7 17,629 - -2.1 17,833 + Psest_0017 0.31 +0.2 17,833 + Psest_0017 0.31 -2.5 17,833 + Psest_0017 0.31 -0.7 17,834 - Psest_0017 0.31 -0.2 17,834 - Psest_0017 0.31 -0.3 17,834 - Psest_0017 0.31 -1.4 17,834 - Psest_0017 0.31 +0.0 17,983 + Psest_0017 0.53 +1.7 17,983 + Psest_0017 0.53 +1.0 17,983 + Psest_0017 0.53 +0.0 17,983 + Psest_0017 0.53 -1.3 17,984 - Psest_0017 0.53 +0.4 17,992 + Psest_0017 0.55 +0.8 18,046 + Psest_0017 0.63 +3.5 18,047 - Psest_0017 0.63 +2.0 18,057 + Psest_0017 0.65 +0.1 18,057 + Psest_0017 0.65 +0.1 18,058 - Psest_0017 0.65 +0.4 18,058 - Psest_0017 0.65 +1.1 18,058 - Psest_0017 0.65 +0.3 18,058 - Psest_0017 0.65 -0.6 18,061 + Psest_0017 0.65 -0.1 18,061 + Psest_0017 0.65 -2.0 18,061 + Psest_0017 0.65 +0.3 18,061 + Psest_0017 0.65 -0.6 18,062 - Psest_0017 0.65 +0.2 18,062 - Psest_0017 0.65 -0.3 18,062 - Psest_0017 0.65 +1.0 18,062 - Psest_0017 0.65 -0.5 18,062 - Psest_0017 0.65 -1.4 18,062 - Psest_0017 0.65 -0.5 18,062 - Psest_0017 0.65 +0.1 18,062 - Psest_0017 0.65 -0.7 18,062 - Psest_0017 0.65 -1.5 18,062 - Psest_0017 0.65 +0.7 18,062 - Psest_0017 0.65 -0.0 18,062 - Psest_0017 0.65 +0.3 18,073 + Psest_0017 0.67 +1.0 18,073 + Psest_0017 0.67 +0.2 18,074 - Psest_0017 0.67 +0.5 18,074 - Psest_0017 0.67 -1.7 18,375 + Psest_0018 0.13 -0.2 18,375 + Psest_0018 0.13 -0.2 18,375 + Psest_0018 0.13 -2.0 18,375 + Psest_0018 0.13 +0.5 18,375 + Psest_0018 0.13 +0.7 18,375 + Psest_0018 0.13 -0.9 18,375 + Psest_0018 0.13 -1.8 18,375 + Psest_0018 0.13 +0.2 18,376 - Psest_0018 0.13 +0.6 18,376 - Psest_0018 0.13 +2.3 18,376 - Psest_0018 0.13 +1.8 18,376 - Psest_0018 0.13 -0.1 18,376 - Psest_0018 0.13 +0.6 18,376 - Psest_0018 0.13 -0.3
Or see this region's nucleotide sequence