Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2438

Experiment: L-Lysine 200 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPsest_2436 and Psest_2437 are separated by 47 nucleotidesPsest_2437 and Psest_2438 are separated by 36 nucleotidesPsest_2438 and Psest_2439 are separated by 2 nucleotides Psest_2436: Psest_2436 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data), at 2,603,933 to 2,605,444 _2436 Psest_2437: Psest_2437 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (from data), at 2,605,492 to 2,606,265 _2437 Psest_2438: Psest_2438 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) (from data), at 2,606,302 to 2,607,192 _2438 Psest_2439: Psest_2439 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data), at 2,607,195 to 2,608,289 _2439 Position (kb) 2606 2607 2608Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2605.358 kb on + strandat 2605.358 kb on + strandat 2605.359 kb on - strandat 2605.359 kb on - strandat 2605.359 kb on - strandat 2605.359 kb on - strandat 2605.542 kb on + strandat 2605.714 kb on + strand, within Psest_2437at 2605.726 kb on + strand, within Psest_2437at 2605.727 kb on - strand, within Psest_2437at 2605.727 kb on - strand, within Psest_2437at 2605.898 kb on + strand, within Psest_2437at 2606.280 kb on + strandat 2606.316 kb on + strandat 2606.317 kb on - strandat 2606.524 kb on + strand, within Psest_2438at 2606.524 kb on + strand, within Psest_2438at 2606.524 kb on + strand, within Psest_2438at 2606.956 kb on + strand, within Psest_2438at 2606.956 kb on + strand, within Psest_2438at 2606.956 kb on + strand, within Psest_2438at 2606.956 kb on + strand, within Psest_2438at 2606.956 kb on + strand, within Psest_2438at 2606.956 kb on + strand, within Psest_2438at 2606.956 kb on + strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2606.957 kb on - strand, within Psest_2438at 2607.004 kb on + strand, within Psest_2438at 2607.230 kb on - strandat 2607.468 kb on + strand, within Psest_2439at 2607.469 kb on - strand, within Psest_2439at 2607.483 kb on + strand, within Psest_2439at 2607.483 kb on + strand, within Psest_2439at 2607.484 kb on - strand, within Psest_2439at 2607.602 kb on + strand, within Psest_2439at 2607.625 kb on - strand, within Psest_2439at 2607.648 kb on + strand, within Psest_2439at 2607.648 kb on + strand, within Psest_2439at 2607.648 kb on + strand, within Psest_2439at 2607.649 kb on - strand, within Psest_2439at 2607.684 kb on + strand, within Psest_2439at 2607.684 kb on + strand, within Psest_2439at 2607.684 kb on + strand, within Psest_2439at 2607.684 kb on + strand, within Psest_2439at 2607.684 kb on + strand, within Psest_2439at 2607.684 kb on + strand, within Psest_2439at 2607.684 kb on + strand, within Psest_2439at 2607.684 kb on + strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439at 2607.685 kb on - strand, within Psest_2439

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction L-Lysine 200 mM
remove
2,605,358 + +1.9
2,605,358 + -1.3
2,605,359 - -0.8
2,605,359 - +0.4
2,605,359 - -0.5
2,605,359 - -0.0
2,605,542 + +0.6
2,605,714 + Psest_2437 0.29 +0.8
2,605,726 + Psest_2437 0.30 +1.1
2,605,727 - Psest_2437 0.30 +0.0
2,605,727 - Psest_2437 0.30 -1.0
2,605,898 + Psest_2437 0.52 -0.3
2,606,280 + -1.1
2,606,316 + +0.8
2,606,317 - -0.3
2,606,524 + Psest_2438 0.25 +0.9
2,606,524 + Psest_2438 0.25 -1.6
2,606,524 + Psest_2438 0.25 -0.1
2,606,956 + Psest_2438 0.73 -0.3
2,606,956 + Psest_2438 0.73 -3.3
2,606,956 + Psest_2438 0.73 +0.3
2,606,956 + Psest_2438 0.73 +0.3
2,606,956 + Psest_2438 0.73 +0.6
2,606,956 + Psest_2438 0.73 -0.6
2,606,956 + Psest_2438 0.73 -0.7
2,606,957 - Psest_2438 0.74 +0.7
2,606,957 - Psest_2438 0.74 +0.2
2,606,957 - Psest_2438 0.74 -1.8
2,606,957 - Psest_2438 0.74 -0.6
2,606,957 - Psest_2438 0.74 +0.5
2,606,957 - Psest_2438 0.74 +1.0
2,606,957 - Psest_2438 0.74 -0.2
2,606,957 - Psest_2438 0.74 -1.9
2,606,957 - Psest_2438 0.74 -0.2
2,606,957 - Psest_2438 0.74 -1.6
2,606,957 - Psest_2438 0.74 -0.7
2,606,957 - Psest_2438 0.74 -1.8
2,606,957 - Psest_2438 0.74 -2.0
2,606,957 - Psest_2438 0.74 -0.1
2,606,957 - Psest_2438 0.74 -0.5
2,607,004 + Psest_2438 0.79 -0.5
2,607,230 - -1.0
2,607,468 + Psest_2439 0.25 +0.8
2,607,469 - Psest_2439 0.25 -0.2
2,607,483 + Psest_2439 0.26 -1.6
2,607,483 + Psest_2439 0.26 -0.1
2,607,484 - Psest_2439 0.26 +0.1
2,607,602 + Psest_2439 0.37 +0.9
2,607,625 - Psest_2439 0.39 +0.9
2,607,648 + Psest_2439 0.41 -0.5
2,607,648 + Psest_2439 0.41 -0.2
2,607,648 + Psest_2439 0.41 -2.7
2,607,649 - Psest_2439 0.41 -1.2
2,607,684 + Psest_2439 0.45 -1.6
2,607,684 + Psest_2439 0.45 -1.5
2,607,684 + Psest_2439 0.45 +2.4
2,607,684 + Psest_2439 0.45 +1.0
2,607,684 + Psest_2439 0.45 +0.5
2,607,684 + Psest_2439 0.45 +1.2
2,607,684 + Psest_2439 0.45 -0.6
2,607,684 + Psest_2439 0.45 -1.3
2,607,685 - Psest_2439 0.45 -0.2
2,607,685 - Psest_2439 0.45 -0.4
2,607,685 - Psest_2439 0.45 -1.4
2,607,685 - Psest_2439 0.45 +0.8
2,607,685 - Psest_2439 0.45 -0.0
2,607,685 - Psest_2439 0.45 -0.9
2,607,685 - Psest_2439 0.45 +0.3
2,607,685 - Psest_2439 0.45 +1.8
2,607,685 - Psest_2439 0.45 -1.9
2,607,685 - Psest_2439 0.45 +0.5
2,607,685 - Psest_2439 0.45 -0.6
2,607,685 - Psest_2439 0.45 -0.7
2,607,685 - Psest_2439 0.45 +2.6
2,607,685 - Psest_2439 0.45 +0.3

Or see this region's nucleotide sequence