Experiment: L-Lysine 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0185 and Psest_0186 are separated by 183 nucleotides Psest_0186 and Psest_0187 are separated by 6 nucleotides Psest_0187 and Psest_0188 overlap by 4 nucleotides
Psest_0185: Psest_0185 - Cytochrome c553, at 180,926 to 181,558
_0185
Psest_0186: Psest_0186 - Protein-disulfide isomerase, at 181,742 to 182,371
_0186
Psest_0187: Psest_0187 - Metal-dependent hydrolase, at 182,378 to 183,247
_0187
Psest_0188: Psest_0188 - diguanylate cyclase (GGDEF) domain, at 183,244 to 185,142
_0188
Position (kb) 182
183
184 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 181.418 kb on + strand, within Psest_0185 at 181.419 kb on - strand, within Psest_0185 at 181.538 kb on + strand at 181.569 kb on - strand at 181.598 kb on + strand at 181.599 kb on - strand at 181.599 kb on - strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.736 kb on + strand at 181.832 kb on + strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 181.833 kb on - strand, within Psest_0186 at 182.016 kb on + strand, within Psest_0186 at 182.016 kb on + strand, within Psest_0186 at 182.017 kb on - strand, within Psest_0186 at 182.017 kb on - strand, within Psest_0186 at 182.017 kb on - strand, within Psest_0186 at 182.183 kb on + strand, within Psest_0186 at 182.184 kb on - strand, within Psest_0186 at 182.184 kb on - strand, within Psest_0186 at 182.211 kb on + strand, within Psest_0186 at 182.240 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.282 kb on + strand, within Psest_0186 at 182.534 kb on + strand, within Psest_0187 at 182.534 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.618 kb on + strand, within Psest_0187 at 182.619 kb on - strand, within Psest_0187 at 182.721 kb on - strand, within Psest_0187 at 182.933 kb on + strand, within Psest_0187 at 182.990 kb on - strand, within Psest_0187 at 183.277 kb on + strand at 183.278 kb on - strand at 183.341 kb on + strand at 183.488 kb on + strand, within Psest_0188 at 183.488 kb on + strand, within Psest_0188 at 183.601 kb on - strand, within Psest_0188 at 183.601 kb on - strand, within Psest_0188 at 184.069 kb on + strand, within Psest_0188 at 184.225 kb on + strand, within Psest_0188 at 184.226 kb on - strand, within Psest_0188
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine 200 mM remove 181,418 + Psest_0185 0.78 -0.6 181,419 - Psest_0185 0.78 +1.9 181,538 + +0.3 181,569 - -0.0 181,598 + +1.1 181,599 - +0.7 181,599 - -0.0 181,736 + -2.2 181,736 + +1.4 181,736 + -0.4 181,736 + -1.9 181,736 + -1.3 181,736 + +0.0 181,736 + +1.8 181,736 + -0.4 181,832 + Psest_0186 0.14 -0.9 181,833 - Psest_0186 0.14 +3.2 181,833 - Psest_0186 0.14 -1.1 181,833 - Psest_0186 0.14 +0.2 181,833 - Psest_0186 0.14 -1.4 182,016 + Psest_0186 0.43 -0.3 182,016 + Psest_0186 0.43 -0.7 182,017 - Psest_0186 0.44 -1.0 182,017 - Psest_0186 0.44 +0.8 182,017 - Psest_0186 0.44 -1.6 182,183 + Psest_0186 0.70 +0.7 182,184 - Psest_0186 0.70 -1.3 182,184 - Psest_0186 0.70 +0.3 182,211 + Psest_0186 0.74 -1.0 182,240 + Psest_0186 0.79 -0.2 182,282 + Psest_0186 0.86 -2.0 182,282 + Psest_0186 0.86 -1.4 182,282 + Psest_0186 0.86 +0.1 182,282 + Psest_0186 0.86 -0.7 182,282 + Psest_0186 0.86 +0.8 182,534 + Psest_0187 0.18 +2.7 182,534 + Psest_0187 0.18 +1.2 182,618 + Psest_0187 0.28 +1.3 182,618 + Psest_0187 0.28 +1.6 182,618 + Psest_0187 0.28 +0.6 182,618 + Psest_0187 0.28 -1.3 182,619 - Psest_0187 0.28 +1.4 182,721 - Psest_0187 0.39 -2.0 182,933 + Psest_0187 0.64 +2.2 182,990 - Psest_0187 0.70 -1.3 183,277 + -0.7 183,278 - +1.5 183,341 + +1.3 183,488 + Psest_0188 0.13 -1.7 183,488 + Psest_0188 0.13 +0.7 183,601 - Psest_0188 0.19 -0.2 183,601 - Psest_0188 0.19 -0.3 184,069 + Psest_0188 0.43 +0.5 184,225 + Psest_0188 0.52 +0.7 184,226 - Psest_0188 0.52 -1.6
Or see this region's nucleotide sequence