Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3741 and Psest_3742 overlap by 25 nucleotides Psest_3742 and Psest_3743 are separated by 104 nucleotides Psest_3743 and Psest_3744 are separated by 257 nucleotides Psest_3744 and Psest_3745 are separated by 117 nucleotides
Psest_3741: Psest_3741 - TIGR02444 family protein, at 3,912,962 to 3,913,438
_3741
Psest_3742: Psest_3742 - hypothetical protein, at 3,913,414 to 3,914,244
_3742
Psest_3743: Psest_3743 - Regulator of sigma D, at 3,914,349 to 3,914,816
_3743
Psest_3744: Psest_3744 - Disulfide bond formation protein DsbB, at 3,915,074 to 3,915,565
_3744
Psest_3745: Psest_3745 - Uncharacterized enzyme of heme biosynthesis, at 3,915,683 to 3,916,903
_3745
Position (kb)
3914
3915 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3913.855 kb on + strand, within Psest_3742 at 3913.856 kb on - strand, within Psest_3742 at 3913.856 kb on - strand, within Psest_3742 at 3913.943 kb on + strand, within Psest_3742 at 3913.943 kb on + strand, within Psest_3742 at 3913.943 kb on + strand, within Psest_3742 at 3913.943 kb on + strand, within Psest_3742 at 3913.943 kb on + strand, within Psest_3742 at 3913.944 kb on - strand, within Psest_3742 at 3914.217 kb on + strand at 3914.217 kb on + strand at 3914.277 kb on + strand at 3914.277 kb on + strand at 3914.277 kb on + strand at 3914.280 kb on + strand at 3914.280 kb on + strand at 3914.281 kb on - strand at 3914.281 kb on - strand at 3914.281 kb on - strand at 3914.360 kb on + strand at 3914.546 kb on - strand, within Psest_3743 at 3914.615 kb on - strand, within Psest_3743 at 3914.641 kb on + strand, within Psest_3743 at 3914.641 kb on + strand, within Psest_3743 at 3914.642 kb on - strand, within Psest_3743 at 3914.642 kb on - strand, within Psest_3743 at 3914.653 kb on + strand, within Psest_3743 at 3914.766 kb on + strand, within Psest_3743 at 3914.766 kb on + strand, within Psest_3743 at 3914.766 kb on + strand, within Psest_3743 at 3914.766 kb on + strand, within Psest_3743 at 3914.766 kb on + strand, within Psest_3743 at 3914.767 kb on - strand, within Psest_3743 at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.772 kb on + strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.773 kb on - strand at 3914.834 kb on - strand at 3914.849 kb on - strand at 3914.940 kb on + strand at 3914.940 kb on + strand at 3914.941 kb on - strand at 3915.063 kb on - strand at 3915.246 kb on + strand, within Psest_3744 at 3915.246 kb on + strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.247 kb on - strand, within Psest_3744 at 3915.341 kb on + strand, within Psest_3744 at 3915.360 kb on + strand, within Psest_3744 at 3915.360 kb on + strand, within Psest_3744 at 3915.361 kb on - strand, within Psest_3744 at 3915.361 kb on - strand, within Psest_3744 at 3915.361 kb on - strand, within Psest_3744 at 3915.489 kb on + strand, within Psest_3744 at 3915.489 kb on + strand, within Psest_3744 at 3915.490 kb on - strand, within Psest_3744 at 3915.490 kb on - strand, within Psest_3744 at 3915.490 kb on - strand, within Psest_3744 at 3915.490 kb on - strand, within Psest_3744 at 3915.490 kb on - strand, within Psest_3744 at 3915.594 kb on + strand at 3915.595 kb on - strand at 3915.620 kb on + strand at 3915.620 kb on + strand at 3915.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 3,913,855 + Psest_3742 0.53 -1.6 3,913,856 - Psest_3742 0.53 +0.5 3,913,856 - Psest_3742 0.53 -1.8 3,913,943 + Psest_3742 0.64 -0.2 3,913,943 + Psest_3742 0.64 -0.5 3,913,943 + Psest_3742 0.64 +0.3 3,913,943 + Psest_3742 0.64 -1.7 3,913,943 + Psest_3742 0.64 +1.0 3,913,944 - Psest_3742 0.64 -1.3 3,914,217 + +1.1 3,914,217 + -2.1 3,914,277 + +3.7 3,914,277 + -0.0 3,914,277 + +1.3 3,914,280 + -0.2 3,914,280 + -2.6 3,914,281 - -1.0 3,914,281 - -1.1 3,914,281 - +0.0 3,914,360 + -0.2 3,914,546 - Psest_3743 0.42 -1.1 3,914,615 - Psest_3743 0.57 +1.0 3,914,641 + Psest_3743 0.62 -0.3 3,914,641 + Psest_3743 0.62 +0.2 3,914,642 - Psest_3743 0.63 -1.0 3,914,642 - Psest_3743 0.63 -0.5 3,914,653 + Psest_3743 0.65 -1.3 3,914,766 + Psest_3743 0.89 -1.0 3,914,766 + Psest_3743 0.89 -2.9 3,914,766 + Psest_3743 0.89 -1.9 3,914,766 + Psest_3743 0.89 -1.5 3,914,766 + Psest_3743 0.89 +0.1 3,914,767 - Psest_3743 0.89 +0.1 3,914,772 + -0.3 3,914,772 + -0.2 3,914,772 + +1.0 3,914,772 + -0.9 3,914,772 + +1.0 3,914,772 + +2.0 3,914,772 + -2.3 3,914,773 - -2.2 3,914,773 - -2.0 3,914,773 - -0.0 3,914,773 - +0.9 3,914,773 - -1.5 3,914,773 - -0.4 3,914,773 - -1.1 3,914,773 - -0.2 3,914,834 - -1.7 3,914,849 - -0.6 3,914,940 + -1.9 3,914,940 + -1.8 3,914,941 - +0.3 3,915,063 - -1.0 3,915,246 + Psest_3744 0.35 -1.3 3,915,246 + Psest_3744 0.35 -0.1 3,915,247 - Psest_3744 0.35 -1.7 3,915,247 - Psest_3744 0.35 -0.6 3,915,247 - Psest_3744 0.35 -1.4 3,915,247 - Psest_3744 0.35 -0.3 3,915,247 - Psest_3744 0.35 -1.4 3,915,247 - Psest_3744 0.35 -0.6 3,915,341 + Psest_3744 0.54 -1.9 3,915,360 + Psest_3744 0.58 -1.4 3,915,360 + Psest_3744 0.58 -1.9 3,915,361 - Psest_3744 0.58 -0.3 3,915,361 - Psest_3744 0.58 -0.8 3,915,361 - Psest_3744 0.58 -2.4 3,915,489 + Psest_3744 0.84 -1.1 3,915,489 + Psest_3744 0.84 -0.7 3,915,490 - Psest_3744 0.85 -3.5 3,915,490 - Psest_3744 0.85 -2.8 3,915,490 - Psest_3744 0.85 -1.3 3,915,490 - Psest_3744 0.85 -0.3 3,915,490 - Psest_3744 0.85 -0.2 3,915,594 + -2.3 3,915,595 - +0.2 3,915,620 + -0.3 3,915,620 + -1.7 3,915,621 - -1.7
Or see this region's nucleotide sequence