Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1910 and Psest_1911 are separated by 187 nucleotides Psest_1911 and Psest_1912 are separated by 327 nucleotides Psest_1912 and Psest_1913 are separated by 6 nucleotides
Psest_1910: Psest_1910 - 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase, at 2,017,763 to 2,018,647
_1910
Psest_1911: Psest_1911 - spermidine synthase, at 2,018,835 to 2,019,695
_1911
Psest_1912: Psest_1912 - Protein of unknown function (DUF1260)., at 2,020,023 to 2,020,364
_1912
Psest_1913: Psest_1913 - Transcriptional regulator, at 2,020,371 to 2,021,327
_1913
Position (kb)
2018
2019
2020 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2017.959 kb on + strand, within Psest_1910 at 2017.960 kb on - strand, within Psest_1910 at 2017.960 kb on - strand, within Psest_1910 at 2018.078 kb on + strand, within Psest_1910 at 2018.405 kb on + strand, within Psest_1910 at 2018.405 kb on + strand, within Psest_1910 at 2018.406 kb on - strand, within Psest_1910 at 2018.406 kb on - strand, within Psest_1910 at 2018.647 kb on + strand at 2018.659 kb on - strand at 2018.734 kb on + strand at 2018.734 kb on + strand at 2018.735 kb on - strand at 2018.735 kb on - strand at 2019.012 kb on + strand, within Psest_1911 at 2019.013 kb on - strand, within Psest_1911 at 2019.013 kb on - strand, within Psest_1911 at 2019.013 kb on - strand, within Psest_1911 at 2019.013 kb on - strand, within Psest_1911 at 2019.348 kb on + strand, within Psest_1911 at 2019.480 kb on + strand, within Psest_1911 at 2019.481 kb on - strand, within Psest_1911 at 2019.481 kb on - strand, within Psest_1911 at 2019.481 kb on - strand, within Psest_1911 at 2019.497 kb on + strand, within Psest_1911 at 2019.497 kb on + strand, within Psest_1911 at 2019.497 kb on + strand, within Psest_1911 at 2019.498 kb on - strand, within Psest_1911 at 2019.498 kb on - strand, within Psest_1911 at 2019.498 kb on - strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.501 kb on + strand, within Psest_1911 at 2019.502 kb on - strand, within Psest_1911 at 2019.502 kb on - strand, within Psest_1911 at 2019.502 kb on - strand, within Psest_1911 at 2019.612 kb on + strand at 2019.613 kb on - strand at 2019.615 kb on + strand at 2019.615 kb on + strand at 2019.615 kb on + strand at 2019.615 kb on + strand at 2019.616 kb on - strand at 2019.616 kb on - strand at 2019.658 kb on - strand at 2019.658 kb on - strand at 2019.658 kb on - strand at 2019.658 kb on - strand at 2019.658 kb on - strand at 2019.883 kb on + strand at 2019.884 kb on - strand at 2019.884 kb on - strand at 2019.884 kb on - strand at 2019.924 kb on + strand at 2020.005 kb on - strand at 2020.005 kb on - strand at 2020.005 kb on - strand at 2020.119 kb on + strand, within Psest_1912 at 2020.120 kb on - strand, within Psest_1912 at 2020.187 kb on + strand, within Psest_1912 at 2020.231 kb on + strand, within Psest_1912 at 2020.245 kb on + strand, within Psest_1912 at 2020.245 kb on + strand, within Psest_1912 at 2020.245 kb on + strand, within Psest_1912 at 2020.245 kb on + strand, within Psest_1912 at 2020.246 kb on - strand, within Psest_1912 at 2020.246 kb on - strand, within Psest_1912 at 2020.246 kb on - strand, within Psest_1912 at 2020.246 kb on - strand, within Psest_1912 at 2020.246 kb on - strand, within Psest_1912 at 2020.246 kb on - strand, within Psest_1912 at 2020.246 kb on - strand, within Psest_1912 at 2020.496 kb on - strand, within Psest_1913
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 2,017,959 + Psest_1910 0.22 -1.5 2,017,960 - Psest_1910 0.22 -3.0 2,017,960 - Psest_1910 0.22 +0.3 2,018,078 + Psest_1910 0.36 +0.8 2,018,405 + Psest_1910 0.73 +1.0 2,018,405 + Psest_1910 0.73 +0.2 2,018,406 - Psest_1910 0.73 +0.8 2,018,406 - Psest_1910 0.73 +1.1 2,018,647 + +1.1 2,018,659 - +1.4 2,018,734 + -0.4 2,018,734 + +0.0 2,018,735 - -0.6 2,018,735 - -0.6 2,019,012 + Psest_1911 0.21 -0.2 2,019,013 - Psest_1911 0.21 +0.1 2,019,013 - Psest_1911 0.21 +1.1 2,019,013 - Psest_1911 0.21 +1.9 2,019,013 - Psest_1911 0.21 +1.5 2,019,348 + Psest_1911 0.60 +0.5 2,019,480 + Psest_1911 0.75 -0.7 2,019,481 - Psest_1911 0.75 -0.7 2,019,481 - Psest_1911 0.75 -0.5 2,019,481 - Psest_1911 0.75 +0.9 2,019,497 + Psest_1911 0.77 -0.3 2,019,497 + Psest_1911 0.77 -0.6 2,019,497 + Psest_1911 0.77 -0.4 2,019,498 - Psest_1911 0.77 +1.6 2,019,498 - Psest_1911 0.77 -0.6 2,019,498 - Psest_1911 0.77 +0.9 2,019,501 + Psest_1911 0.77 -0.3 2,019,501 + Psest_1911 0.77 +1.3 2,019,501 + Psest_1911 0.77 +0.6 2,019,501 + Psest_1911 0.77 +0.0 2,019,501 + Psest_1911 0.77 -1.2 2,019,501 + Psest_1911 0.77 +0.4 2,019,502 - Psest_1911 0.77 -2.0 2,019,502 - Psest_1911 0.77 +0.2 2,019,502 - Psest_1911 0.77 -1.1 2,019,612 + +1.7 2,019,613 - -2.4 2,019,615 + -1.8 2,019,615 + +1.3 2,019,615 + +0.2 2,019,615 + -0.1 2,019,616 - -1.2 2,019,616 - -2.6 2,019,658 - -0.4 2,019,658 - +0.3 2,019,658 - +0.7 2,019,658 - -0.8 2,019,658 - +0.9 2,019,883 + +0.2 2,019,884 - +2.3 2,019,884 - +0.4 2,019,884 - +1.2 2,019,924 + +0.1 2,020,005 - -0.8 2,020,005 - -0.4 2,020,005 - +0.4 2,020,119 + Psest_1912 0.28 -0.8 2,020,120 - Psest_1912 0.28 +0.0 2,020,187 + Psest_1912 0.48 +0.8 2,020,231 + Psest_1912 0.61 +0.6 2,020,245 + Psest_1912 0.65 +1.2 2,020,245 + Psest_1912 0.65 -0.7 2,020,245 + Psest_1912 0.65 -1.6 2,020,245 + Psest_1912 0.65 -1.5 2,020,246 - Psest_1912 0.65 -0.3 2,020,246 - Psest_1912 0.65 -2.0 2,020,246 - Psest_1912 0.65 -0.1 2,020,246 - Psest_1912 0.65 -1.2 2,020,246 - Psest_1912 0.65 -0.2 2,020,246 - Psest_1912 0.65 +0.5 2,020,246 - Psest_1912 0.65 -0.8 2,020,496 - Psest_1913 0.13 -1.1
Or see this region's nucleotide sequence