Experiment: Choline Lysine 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1887 and Psest_1888 are separated by 13 nucleotides Psest_1888 and Psest_1889 are separated by 46 nucleotides Psest_1889 and Psest_1890 are separated by 28 nucleotides Psest_1890 and Psest_1891 are separated by 19 nucleotides
Psest_1887: Psest_1887 - Predicted metal-dependent phosphoesterases (PHP family), at 1,996,181 to 1,997,047
_1887
Psest_1888: Psest_1888 - Sua5/YciO/YrdC/YwlC family protein, at 1,997,061 to 1,997,684
_1888
Psest_1889: Psest_1889 - tryptophanyl-tRNA synthetase, at 1,997,731 to 1,998,945
_1889
Psest_1890: Psest_1890 - Uncharacterized conserved protein, at 1,998,974 to 1,999,795
_1890
Psest_1891: Psest_1891 - segregation and condensation protein B, at 1,999,815 to 2,000,585
_1891
Position (kb)
1997
1998
1999 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1996.897 kb on - strand, within Psest_1887 at 1997.564 kb on - strand, within Psest_1888 at 1997.642 kb on - strand at 1997.642 kb on - strand at 1997.711 kb on + strand at 1997.712 kb on - strand at 1997.712 kb on - strand at 1997.712 kb on - strand at 1997.718 kb on + strand at 1997.718 kb on + strand at 1997.719 kb on - strand at 1997.719 kb on - strand at 1997.803 kb on + strand at 1997.803 kb on + strand at 1997.932 kb on - strand, within Psest_1889 at 1997.981 kb on + strand, within Psest_1889 at 1998.074 kb on - strand, within Psest_1889 at 1998.074 kb on - strand, within Psest_1889 at 1998.074 kb on - strand, within Psest_1889 at 1998.115 kb on - strand, within Psest_1889 at 1998.119 kb on - strand, within Psest_1889 at 1998.119 kb on - strand, within Psest_1889 at 1998.166 kb on + strand, within Psest_1889 at 1998.167 kb on - strand, within Psest_1889 at 1998.253 kb on + strand, within Psest_1889 at 1998.254 kb on - strand, within Psest_1889 at 1998.329 kb on - strand, within Psest_1889 at 1998.329 kb on - strand, within Psest_1889 at 1998.350 kb on - strand, within Psest_1889 at 1998.443 kb on - strand, within Psest_1889 at 1998.565 kb on + strand, within Psest_1889 at 1998.565 kb on + strand, within Psest_1889 at 1998.566 kb on - strand, within Psest_1889 at 1998.566 kb on - strand, within Psest_1889 at 1998.566 kb on - strand, within Psest_1889 at 1998.566 kb on - strand, within Psest_1889 at 1998.632 kb on + strand, within Psest_1889 at 1998.632 kb on + strand, within Psest_1889 at 1998.632 kb on + strand, within Psest_1889 at 1998.633 kb on - strand, within Psest_1889 at 1998.633 kb on - strand, within Psest_1889 at 1998.826 kb on + strand at 1998.827 kb on - strand at 1999.026 kb on + strand at 1999.043 kb on + strand at 1999.044 kb on - strand at 1999.079 kb on + strand, within Psest_1890 at 1999.079 kb on + strand, within Psest_1890 at 1999.079 kb on + strand, within Psest_1890 at 1999.079 kb on + strand, within Psest_1890 at 1999.079 kb on + strand, within Psest_1890 at 1999.080 kb on - strand, within Psest_1890 at 1999.080 kb on - strand, within Psest_1890 at 1999.080 kb on - strand, within Psest_1890 at 1999.145 kb on + strand, within Psest_1890 at 1999.145 kb on + strand, within Psest_1890 at 1999.146 kb on - strand, within Psest_1890 at 1999.146 kb on - strand, within Psest_1890 at 1999.146 kb on - strand, within Psest_1890 at 1999.180 kb on + strand, within Psest_1890 at 1999.208 kb on + strand, within Psest_1890 at 1999.208 kb on + strand, within Psest_1890 at 1999.208 kb on + strand, within Psest_1890 at 1999.208 kb on + strand, within Psest_1890 at 1999.208 kb on + strand, within Psest_1890 at 1999.208 kb on + strand, within Psest_1890 at 1999.209 kb on - strand, within Psest_1890 at 1999.209 kb on - strand, within Psest_1890 at 1999.209 kb on - strand, within Psest_1890 at 1999.209 kb on - strand, within Psest_1890 at 1999.209 kb on - strand, within Psest_1890 at 1999.209 kb on - strand, within Psest_1890 at 1999.263 kb on - strand, within Psest_1890 at 1999.263 kb on - strand, within Psest_1890 at 1999.552 kb on + strand, within Psest_1890 at 1999.553 kb on - strand, within Psest_1890
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 20 mM remove 1,996,897 - Psest_1887 0.83 -0.5 1,997,564 - Psest_1888 0.81 +1.4 1,997,642 - +0.3 1,997,642 - +1.1 1,997,711 + -0.4 1,997,712 - -0.7 1,997,712 - +1.2 1,997,712 - -0.6 1,997,718 + +0.8 1,997,718 + -1.1 1,997,719 - +0.1 1,997,719 - +1.0 1,997,803 + +0.2 1,997,803 + +1.5 1,997,932 - Psest_1889 0.17 +2.2 1,997,981 + Psest_1889 0.21 +2.2 1,998,074 - Psest_1889 0.28 +1.6 1,998,074 - Psest_1889 0.28 +1.4 1,998,074 - Psest_1889 0.28 -0.7 1,998,115 - Psest_1889 0.32 -0.2 1,998,119 - Psest_1889 0.32 -0.1 1,998,119 - Psest_1889 0.32 +0.2 1,998,166 + Psest_1889 0.36 +0.7 1,998,167 - Psest_1889 0.36 +3.3 1,998,253 + Psest_1889 0.43 +1.5 1,998,254 - Psest_1889 0.43 +1.3 1,998,329 - Psest_1889 0.49 +1.8 1,998,329 - Psest_1889 0.49 +0.5 1,998,350 - Psest_1889 0.51 +0.8 1,998,443 - Psest_1889 0.59 +2.0 1,998,565 + Psest_1889 0.69 +1.2 1,998,565 + Psest_1889 0.69 -3.0 1,998,566 - Psest_1889 0.69 +0.8 1,998,566 - Psest_1889 0.69 +4.4 1,998,566 - Psest_1889 0.69 -0.6 1,998,566 - Psest_1889 0.69 +1.7 1,998,632 + Psest_1889 0.74 +0.4 1,998,632 + Psest_1889 0.74 -0.8 1,998,632 + Psest_1889 0.74 +0.6 1,998,633 - Psest_1889 0.74 -1.8 1,998,633 - Psest_1889 0.74 +0.4 1,998,826 + +0.5 1,998,827 - +0.5 1,999,026 + +1.7 1,999,043 + -1.0 1,999,044 - +1.1 1,999,079 + Psest_1890 0.13 -0.3 1,999,079 + Psest_1890 0.13 +1.9 1,999,079 + Psest_1890 0.13 -0.5 1,999,079 + Psest_1890 0.13 -0.9 1,999,079 + Psest_1890 0.13 -1.4 1,999,080 - Psest_1890 0.13 +0.4 1,999,080 - Psest_1890 0.13 +3.6 1,999,080 - Psest_1890 0.13 +0.5 1,999,145 + Psest_1890 0.21 -0.2 1,999,145 + Psest_1890 0.21 +0.1 1,999,146 - Psest_1890 0.21 +0.9 1,999,146 - Psest_1890 0.21 +1.1 1,999,146 - Psest_1890 0.21 -0.4 1,999,180 + Psest_1890 0.25 +2.3 1,999,208 + Psest_1890 0.28 -1.6 1,999,208 + Psest_1890 0.28 +1.8 1,999,208 + Psest_1890 0.28 -0.0 1,999,208 + Psest_1890 0.28 +1.2 1,999,208 + Psest_1890 0.28 +3.5 1,999,208 + Psest_1890 0.28 +1.7 1,999,209 - Psest_1890 0.29 -0.7 1,999,209 - Psest_1890 0.29 +0.1 1,999,209 - Psest_1890 0.29 +0.2 1,999,209 - Psest_1890 0.29 +0.3 1,999,209 - Psest_1890 0.29 +1.7 1,999,209 - Psest_1890 0.29 -0.1 1,999,263 - Psest_1890 0.35 +1.1 1,999,263 - Psest_1890 0.35 +1.6 1,999,552 + Psest_1890 0.70 -0.3 1,999,553 - Psest_1890 0.70 +1.2
Or see this region's nucleotide sequence