Experiment: 1-ethyl-3-methylimidazolium acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2436 and Psest_2437 are separated by 47 nucleotides Psest_2437 and Psest_2438 are separated by 36 nucleotides Psest_2438 and Psest_2439 are separated by 2 nucleotides
Psest_2436: Psest_2436 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (from data) , at 2,603,933 to 2,605,444
_2436
Psest_2437: Psest_2437 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (from data) , at 2,605,492 to 2,606,265
_2437
Psest_2438: Psest_2438 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) (from data) , at 2,606,302 to 2,607,192
_2438
Psest_2439: Psest_2439 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 2,607,195 to 2,608,289
_2439
Position (kb)
2606
2607
2608 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2605.358 kb on + strand at 2605.358 kb on + strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.359 kb on - strand at 2605.542 kb on + strand at 2605.714 kb on + strand, within Psest_2437 at 2605.726 kb on + strand, within Psest_2437 at 2605.727 kb on - strand, within Psest_2437 at 2605.727 kb on - strand, within Psest_2437 at 2605.898 kb on + strand, within Psest_2437 at 2606.280 kb on + strand at 2606.316 kb on + strand at 2606.317 kb on - strand at 2606.524 kb on + strand, within Psest_2438 at 2606.524 kb on + strand, within Psest_2438 at 2606.524 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2607.004 kb on + strand, within Psest_2438 at 2607.230 kb on - strand at 2607.468 kb on + strand, within Psest_2439 at 2607.469 kb on - strand, within Psest_2439 at 2607.483 kb on + strand, within Psest_2439 at 2607.483 kb on + strand, within Psest_2439 at 2607.484 kb on - strand, within Psest_2439 at 2607.602 kb on + strand, within Psest_2439 at 2607.625 kb on - strand, within Psest_2439 at 2607.648 kb on + strand, within Psest_2439 at 2607.648 kb on + strand, within Psest_2439 at 2607.648 kb on + strand, within Psest_2439 at 2607.649 kb on - strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 40 mM remove 2,605,358 + +0.5 2,605,358 + -1.0 2,605,359 - +0.3 2,605,359 - +0.6 2,605,359 - -0.6 2,605,359 - +0.9 2,605,542 + -1.8 2,605,714 + Psest_2437 0.29 -0.3 2,605,726 + Psest_2437 0.30 +0.8 2,605,727 - Psest_2437 0.30 +0.9 2,605,727 - Psest_2437 0.30 -0.8 2,605,898 + Psest_2437 0.52 +2.0 2,606,280 + +2.6 2,606,316 + -0.8 2,606,317 - -0.4 2,606,524 + Psest_2438 0.25 -1.7 2,606,524 + Psest_2438 0.25 -0.5 2,606,524 + Psest_2438 0.25 +2.1 2,606,956 + Psest_2438 0.73 -1.9 2,606,956 + Psest_2438 0.73 -1.4 2,606,956 + Psest_2438 0.73 +0.7 2,606,956 + Psest_2438 0.73 +0.6 2,606,956 + Psest_2438 0.73 -0.6 2,606,956 + Psest_2438 0.73 -0.1 2,606,956 + Psest_2438 0.73 +0.5 2,606,957 - Psest_2438 0.74 +0.6 2,606,957 - Psest_2438 0.74 +1.7 2,606,957 - Psest_2438 0.74 +0.9 2,606,957 - Psest_2438 0.74 +1.3 2,606,957 - Psest_2438 0.74 +0.9 2,606,957 - Psest_2438 0.74 +2.0 2,606,957 - Psest_2438 0.74 -0.0 2,606,957 - Psest_2438 0.74 +0.6 2,606,957 - Psest_2438 0.74 +0.7 2,606,957 - Psest_2438 0.74 +0.7 2,606,957 - Psest_2438 0.74 -0.7 2,606,957 - Psest_2438 0.74 +0.5 2,606,957 - Psest_2438 0.74 +2.4 2,606,957 - Psest_2438 0.74 +1.9 2,606,957 - Psest_2438 0.74 +1.1 2,607,004 + Psest_2438 0.79 +1.5 2,607,230 - -2.6 2,607,468 + Psest_2439 0.25 -0.6 2,607,469 - Psest_2439 0.25 -1.9 2,607,483 + Psest_2439 0.26 -1.6 2,607,483 + Psest_2439 0.26 +0.5 2,607,484 - Psest_2439 0.26 +0.2 2,607,602 + Psest_2439 0.37 +0.6 2,607,625 - Psest_2439 0.39 -0.2 2,607,648 + Psest_2439 0.41 +0.1 2,607,648 + Psest_2439 0.41 -0.7 2,607,648 + Psest_2439 0.41 +0.0 2,607,649 - Psest_2439 0.41 -1.2 2,607,684 + Psest_2439 0.45 +1.8 2,607,684 + Psest_2439 0.45 -0.2 2,607,684 + Psest_2439 0.45 -1.6 2,607,684 + Psest_2439 0.45 +0.0 2,607,684 + Psest_2439 0.45 -0.3 2,607,684 + Psest_2439 0.45 +1.1 2,607,684 + Psest_2439 0.45 +0.5 2,607,684 + Psest_2439 0.45 +0.3 2,607,685 - Psest_2439 0.45 -1.2 2,607,685 - Psest_2439 0.45 +1.2 2,607,685 - Psest_2439 0.45 -0.4 2,607,685 - Psest_2439 0.45 -0.8 2,607,685 - Psest_2439 0.45 +0.2 2,607,685 - Psest_2439 0.45 -2.9 2,607,685 - Psest_2439 0.45 -0.2 2,607,685 - Psest_2439 0.45 +1.2 2,607,685 - Psest_2439 0.45 +1.0 2,607,685 - Psest_2439 0.45 -0.5 2,607,685 - Psest_2439 0.45 -0.7 2,607,685 - Psest_2439 0.45 -0.4 2,607,685 - Psest_2439 0.45 +1.3 2,607,685 - Psest_2439 0.45 +0.2
Or see this region's nucleotide sequence