Experiment: 1-ethyl-3-methylimidazolium acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1349 and Psest_1350 are separated by 186 nucleotides Psest_1350 and Psest_1351 are separated by 131 nucleotides
Psest_1349: Psest_1349 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 1,459,778 to 1,460,593
_1349
Psest_1350: Psest_1350 - Metal-dependent hydrolases of the beta-lactamase superfamily III, at 1,460,780 to 1,461,772
_1350
Psest_1351: Psest_1351 - hypothetical protein, at 1,461,904 to 1,463,505
_1351
Position (kb)
1460
1461
1462 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1459.780 kb on - strand at 1459.844 kb on + strand at 1459.844 kb on + strand at 1459.845 kb on - strand at 1459.845 kb on - strand at 1459.845 kb on - strand at 1459.845 kb on - strand at 1459.845 kb on - strand at 1460.468 kb on + strand, within Psest_1349 at 1460.468 kb on + strand, within Psest_1349 at 1460.468 kb on + strand, within Psest_1349 at 1460.625 kb on + strand at 1460.625 kb on + strand at 1460.625 kb on + strand at 1460.642 kb on + strand at 1460.643 kb on - strand at 1460.643 kb on - strand at 1460.643 kb on - strand at 1460.647 kb on - strand at 1460.672 kb on + strand at 1460.673 kb on - strand at 1460.826 kb on - strand at 1460.826 kb on - strand at 1460.892 kb on + strand, within Psest_1350 at 1460.892 kb on + strand, within Psest_1350 at 1460.893 kb on - strand, within Psest_1350 at 1461.078 kb on + strand, within Psest_1350 at 1461.078 kb on + strand, within Psest_1350 at 1461.193 kb on - strand, within Psest_1350 at 1461.336 kb on + strand, within Psest_1350 at 1461.336 kb on + strand, within Psest_1350 at 1461.337 kb on - strand, within Psest_1350 at 1461.342 kb on + strand, within Psest_1350 at 1461.584 kb on + strand, within Psest_1350 at 1461.584 kb on + strand, within Psest_1350 at 1461.584 kb on + strand, within Psest_1350 at 1461.584 kb on + strand, within Psest_1350 at 1461.584 kb on + strand, within Psest_1350 at 1461.584 kb on + strand, within Psest_1350 at 1461.584 kb on + strand, within Psest_1350 at 1461.584 kb on + strand, within Psest_1350 at 1461.584 kb on + strand, within Psest_1350 at 1461.584 kb on + strand, within Psest_1350 at 1461.584 kb on + strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.585 kb on - strand, within Psest_1350 at 1461.800 kb on + strand at 1461.800 kb on + strand at 1461.803 kb on + strand at 1461.803 kb on + strand at 1462.194 kb on + strand, within Psest_1351 at 1462.453 kb on + strand, within Psest_1351 at 1462.453 kb on + strand, within Psest_1351 at 1462.453 kb on + strand, within Psest_1351 at 1462.454 kb on - strand, within Psest_1351 at 1462.454 kb on - strand, within Psest_1351 at 1462.454 kb on - strand, within Psest_1351 at 1462.456 kb on + strand, within Psest_1351 at 1462.457 kb on - strand, within Psest_1351 at 1462.524 kb on + strand, within Psest_1351 at 1462.524 kb on + strand, within Psest_1351 at 1462.525 kb on - strand, within Psest_1351 at 1462.525 kb on - strand, within Psest_1351 at 1462.525 kb on - strand, within Psest_1351 at 1462.525 kb on - strand, within Psest_1351 at 1462.534 kb on + strand, within Psest_1351 at 1462.534 kb on + strand, within Psest_1351 at 1462.534 kb on + strand, within Psest_1351 at 1462.546 kb on + strand, within Psest_1351 at 1462.547 kb on - strand, within Psest_1351
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 40 mM remove 1,459,780 - +0.3 1,459,844 + -1.1 1,459,844 + -1.7 1,459,845 - -0.1 1,459,845 - +1.9 1,459,845 - +0.3 1,459,845 - -0.4 1,459,845 - +1.4 1,460,468 + Psest_1349 0.85 -0.0 1,460,468 + Psest_1349 0.85 +1.3 1,460,468 + Psest_1349 0.85 +0.7 1,460,625 + -2.3 1,460,625 + -0.8 1,460,625 + -0.1 1,460,642 + +0.0 1,460,643 - +1.4 1,460,643 - +2.2 1,460,643 - -1.0 1,460,647 - -1.0 1,460,672 + -1.7 1,460,673 - -1.4 1,460,826 - +0.1 1,460,826 - -1.2 1,460,892 + Psest_1350 0.11 -1.0 1,460,892 + Psest_1350 0.11 +1.5 1,460,893 - Psest_1350 0.11 +1.5 1,461,078 + Psest_1350 0.30 -0.5 1,461,078 + Psest_1350 0.30 -0.3 1,461,193 - Psest_1350 0.42 +0.1 1,461,336 + Psest_1350 0.56 -2.3 1,461,336 + Psest_1350 0.56 -0.4 1,461,337 - Psest_1350 0.56 -1.1 1,461,342 + Psest_1350 0.57 -0.6 1,461,584 + Psest_1350 0.81 -0.7 1,461,584 + Psest_1350 0.81 -1.2 1,461,584 + Psest_1350 0.81 -0.4 1,461,584 + Psest_1350 0.81 -0.6 1,461,584 + Psest_1350 0.81 +2.2 1,461,584 + Psest_1350 0.81 +2.0 1,461,584 + Psest_1350 0.81 -0.3 1,461,584 + Psest_1350 0.81 +0.2 1,461,584 + Psest_1350 0.81 -0.4 1,461,584 + Psest_1350 0.81 -0.3 1,461,584 + Psest_1350 0.81 -0.8 1,461,585 - Psest_1350 0.81 -0.1 1,461,585 - Psest_1350 0.81 +0.8 1,461,585 - Psest_1350 0.81 +1.9 1,461,585 - Psest_1350 0.81 +0.9 1,461,585 - Psest_1350 0.81 +0.7 1,461,585 - Psest_1350 0.81 +0.3 1,461,585 - Psest_1350 0.81 -0.8 1,461,585 - Psest_1350 0.81 +0.5 1,461,585 - Psest_1350 0.81 +1.3 1,461,585 - Psest_1350 0.81 -2.8 1,461,585 - Psest_1350 0.81 +0.0 1,461,585 - Psest_1350 0.81 -0.1 1,461,585 - Psest_1350 0.81 +0.8 1,461,585 - Psest_1350 0.81 +1.2 1,461,585 - Psest_1350 0.81 -1.0 1,461,800 + -2.3 1,461,800 + +0.0 1,461,803 + -1.1 1,461,803 + -0.0 1,462,194 + Psest_1351 0.18 -1.3 1,462,453 + Psest_1351 0.34 -2.7 1,462,453 + Psest_1351 0.34 -0.1 1,462,453 + Psest_1351 0.34 +0.4 1,462,454 - Psest_1351 0.34 -1.2 1,462,454 - Psest_1351 0.34 -2.5 1,462,454 - Psest_1351 0.34 -1.4 1,462,456 + Psest_1351 0.34 -0.7 1,462,457 - Psest_1351 0.35 +0.2 1,462,524 + Psest_1351 0.39 -1.0 1,462,524 + Psest_1351 0.39 -1.2 1,462,525 - Psest_1351 0.39 -0.5 1,462,525 - Psest_1351 0.39 -0.8 1,462,525 - Psest_1351 0.39 -0.1 1,462,525 - Psest_1351 0.39 -0.2 1,462,534 + Psest_1351 0.39 -0.7 1,462,534 + Psest_1351 0.39 -0.2 1,462,534 + Psest_1351 0.39 +0.2 1,462,546 + Psest_1351 0.40 +1.3 1,462,547 - Psest_1351 0.40 -1.1
Or see this region's nucleotide sequence