Experiment: Cholin acetate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0507 and Psest_0508 are separated by 216 nucleotides Psest_0508 and Psest_0509 overlap by 1 nucleotides Psest_0509 and Psest_0510 are separated by 15 nucleotides
Psest_0507: Psest_0507 - Uncharacterized membrane-associated protein, at 560,440 to 561,759
_0507
Psest_0508: Psest_0508 - gamma-glutamyl phosphate reductase, at 561,976 to 563,241
_0508
Psest_0509: Psest_0509 - nicotinate (nicotinamide) nucleotide adenylyltransferase, at 563,241 to 563,900
_0509
Psest_0510: Psest_0510 - iojap-like ribosome-associated protein, at 563,916 to 564,275
_0510
Position (kb) 561
562
563
564 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 561.211 kb on + strand, within Psest_0507 at 561.212 kb on - strand, within Psest_0507 at 561.212 kb on - strand, within Psest_0507 at 561.288 kb on - strand, within Psest_0507 at 561.288 kb on - strand, within Psest_0507 at 561.288 kb on - strand, within Psest_0507 at 561.440 kb on + strand, within Psest_0507 at 561.440 kb on + strand, within Psest_0507 at 561.440 kb on + strand, within Psest_0507 at 561.441 kb on - strand, within Psest_0507 at 561.441 kb on - strand, within Psest_0507 at 561.763 kb on + strand at 561.800 kb on + strand at 561.800 kb on + strand at 561.801 kb on - strand at 561.801 kb on - strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.937 kb on + strand at 561.969 kb on + strand at 561.969 kb on + strand at 561.969 kb on + strand at 561.969 kb on + strand at 561.997 kb on + strand at 561.997 kb on + strand at 561.997 kb on + strand at 562.248 kb on + strand, within Psest_0508 at 562.248 kb on + strand, within Psest_0508 at 562.288 kb on + strand, within Psest_0508 at 562.288 kb on + strand, within Psest_0508 at 562.288 kb on + strand, within Psest_0508 at 562.351 kb on + strand, within Psest_0508 at 562.351 kb on + strand, within Psest_0508 at 562.351 kb on + strand, within Psest_0508 at 562.351 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.924 kb on + strand, within Psest_0508 at 562.924 kb on + strand, within Psest_0508 at 563.206 kb on + strand at 563.206 kb on + strand at 563.206 kb on + strand at 563.206 kb on + strand at 563.206 kb on + strand at 563.206 kb on + strand at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.058 kb on + strand, within Psest_0510 at 564.058 kb on + strand, within Psest_0510 at 564.058 kb on + strand, within Psest_0510 at 564.207 kb on + strand, within Psest_0510 at 564.207 kb on + strand, within Psest_0510
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 40 mM remove 561,211 + Psest_0507 0.58 -0.2 561,212 - Psest_0507 0.58 +3.2 561,212 - Psest_0507 0.58 -1.1 561,288 - Psest_0507 0.64 -3.2 561,288 - Psest_0507 0.64 +0.0 561,288 - Psest_0507 0.64 +0.3 561,440 + Psest_0507 0.76 -0.4 561,440 + Psest_0507 0.76 +0.1 561,440 + Psest_0507 0.76 -1.5 561,441 - Psest_0507 0.76 -0.1 561,441 - Psest_0507 0.76 +0.3 561,763 + +2.5 561,800 + -1.5 561,800 + +0.3 561,801 - +0.1 561,801 - +0.2 561,935 + -0.2 561,935 + +0.2 561,935 + +0.8 561,935 + -1.8 561,935 + +0.2 561,935 + -1.7 561,935 + +1.0 561,935 + +0.3 561,935 + +0.5 561,935 + +0.7 561,935 + +2.2 561,935 + -1.3 561,935 + +0.7 561,935 + -0.3 561,935 + +1.2 561,935 + +0.4 561,935 + -0.4 561,935 + -0.3 561,936 - -3.4 561,936 - +0.2 561,936 - -3.3 561,936 - -3.0 561,936 - -0.9 561,936 - +0.7 561,936 - -0.7 561,936 - -2.5 561,936 - -0.6 561,936 - -2.7 561,936 - -3.4 561,936 - -1.6 561,936 - +1.0 561,936 - -2.5 561,936 - -0.9 561,936 - -1.2 561,936 - +0.7 561,936 - -0.6 561,936 - -2.4 561,936 - +0.2 561,936 - -1.3 561,937 + -0.5 561,969 + -0.4 561,969 + +2.8 561,969 + -0.3 561,969 + +0.6 561,997 + -1.6 561,997 + -0.5 561,997 + -2.1 562,248 + Psest_0508 0.21 -0.5 562,248 + Psest_0508 0.21 -0.6 562,288 + Psest_0508 0.25 +0.2 562,288 + Psest_0508 0.25 -2.5 562,288 + Psest_0508 0.25 -1.7 562,351 + Psest_0508 0.30 -3.1 562,351 + Psest_0508 0.30 -2.1 562,351 + Psest_0508 0.30 -2.0 562,351 + Psest_0508 0.30 +0.6 562,678 + Psest_0508 0.55 -2.9 562,678 + Psest_0508 0.55 -1.5 562,678 + Psest_0508 0.55 +0.1 562,678 + Psest_0508 0.55 -0.9 562,678 + Psest_0508 0.55 -1.0 562,678 + Psest_0508 0.55 -3.1 562,678 + Psest_0508 0.55 -0.8 562,678 + Psest_0508 0.55 +0.0 562,678 + Psest_0508 0.55 -1.1 562,678 + Psest_0508 0.55 -0.8 562,678 + Psest_0508 0.55 +0.1 562,678 + Psest_0508 0.55 -2.1 562,924 + Psest_0508 0.75 +1.0 562,924 + Psest_0508 0.75 +0.1 563,206 + -0.7 563,206 + -1.7 563,206 + -1.1 563,206 + +2.1 563,206 + -0.2 563,206 + -1.6 564,021 + Psest_0510 0.29 +0.4 564,021 + Psest_0510 0.29 +0.2 564,021 + Psest_0510 0.29 +0.5 564,021 + Psest_0510 0.29 -2.3 564,021 + Psest_0510 0.29 -0.8 564,021 + Psest_0510 0.29 +1.3 564,021 + Psest_0510 0.29 +0.8 564,021 + Psest_0510 0.29 -0.9 564,058 + Psest_0510 0.39 +0.5 564,058 + Psest_0510 0.39 +1.4 564,058 + Psest_0510 0.39 -0.6 564,207 + Psest_0510 0.81 -0.4 564,207 + Psest_0510 0.81 +0.5
Or see this region's nucleotide sequence