Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_3060

Experiment: Cholin acetate 40 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_3056 and Psest_3057 are separated by 150 nucleotidesPsest_3057 and Psest_3058 are separated by 10 nucleotidesPsest_3058 and Psest_3059 overlap by 4 nucleotidesPsest_3059 and Psest_3060 are separated by 15 nucleotidesPsest_3060 and Psest_3061 are separated by 292 nucleotides Psest_3056: Psest_3056 - hypothetical protein, at 3,237,229 to 3,238,722 _3056 Psest_3057: Psest_3057 - Bacteriophage coat protein B., at 3,238,873 to 3,239,082 _3057 Psest_3058: Psest_3058 - hypothetical protein, at 3,239,093 to 3,239,308 _3058 Psest_3059: Psest_3059 - hypothetical protein, at 3,239,305 to 3,239,484 _3059 Psest_3060: Psest_3060 - hypothetical protein, at 3,239,500 to 3,239,859 _3060 Psest_3061: Psest_3061 - hypothetical protein, at 3,240,152 to 3,240,922 _3061 Position (kb) 3239 3240Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3238.506 kb on + strand, within Psest_3056at 3238.594 kb on - strandat 3238.780 kb on + strandat 3238.780 kb on + strandat 3238.780 kb on + strandat 3238.789 kb on + strandat 3238.865 kb on + strandat 3238.893 kb on - strandat 3238.893 kb on - strandat 3238.893 kb on - strandat 3238.893 kb on - strandat 3238.893 kb on - strandat 3238.893 kb on - strandat 3238.893 kb on - strandat 3239.501 kb on + strandat 3239.692 kb on + strand, within Psest_3060at 3239.692 kb on + strand, within Psest_3060at 3239.693 kb on - strand, within Psest_3060at 3239.693 kb on - strand, within Psest_3060at 3239.705 kb on - strand, within Psest_3060at 3239.790 kb on - strand, within Psest_3060at 3239.790 kb on - strand, within Psest_3060at 3239.963 kb on + strandat 3239.983 kb on + strandat 3239.983 kb on + strandat 3239.983 kb on + strandat 3239.983 kb on + strandat 3239.991 kb on + strandat 3240.014 kb on + strandat 3240.129 kb on + strandat 3240.363 kb on + strand, within Psest_3061at 3240.364 kb on - strand, within Psest_3061at 3240.364 kb on - strand, within Psest_3061at 3240.449 kb on - strand, within Psest_3061at 3240.459 kb on + strand, within Psest_3061at 3240.473 kb on + strand, within Psest_3061at 3240.473 kb on + strand, within Psest_3061at 3240.506 kb on - strand, within Psest_3061at 3240.579 kb on - strand, within Psest_3061at 3240.602 kb on + strand, within Psest_3061at 3240.602 kb on + strand, within Psest_3061at 3240.602 kb on + strand, within Psest_3061at 3240.603 kb on - strand, within Psest_3061at 3240.603 kb on - strand, within Psest_3061at 3240.603 kb on - strand, within Psest_3061at 3240.606 kb on + strand, within Psest_3061at 3240.606 kb on + strand, within Psest_3061at 3240.606 kb on + strand, within Psest_3061at 3240.606 kb on + strand, within Psest_3061at 3240.606 kb on + strand, within Psest_3061at 3240.607 kb on - strand, within Psest_3061at 3240.607 kb on - strand, within Psest_3061at 3240.607 kb on - strand, within Psest_3061at 3240.657 kb on + strand, within Psest_3061at 3240.657 kb on + strand, within Psest_3061at 3240.658 kb on - strand, within Psest_3061at 3240.659 kb on + strand, within Psest_3061at 3240.659 kb on + strand, within Psest_3061at 3240.659 kb on + strand, within Psest_3061at 3240.659 kb on + strand, within Psest_3061at 3240.659 kb on + strand, within Psest_3061at 3240.659 kb on + strand, within Psest_3061at 3240.659 kb on + strand, within Psest_3061at 3240.659 kb on + strand, within Psest_3061at 3240.659 kb on + strand, within Psest_3061at 3240.660 kb on - strand, within Psest_3061at 3240.660 kb on - strand, within Psest_3061at 3240.660 kb on - strand, within Psest_3061at 3240.660 kb on - strand, within Psest_3061at 3240.746 kb on + strand, within Psest_3061

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholin acetate 40 mM
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3,238,506 + Psest_3056 0.85 -0.0
3,238,594 - -0.0
3,238,780 + -1.5
3,238,780 + -1.1
3,238,780 + -1.0
3,238,789 + +0.5
3,238,865 + +1.3
3,238,893 - -0.2
3,238,893 - -2.5
3,238,893 - -0.5
3,238,893 - -1.6
3,238,893 - -1.7
3,238,893 - +0.7
3,238,893 - -0.1
3,239,501 + -1.4
3,239,692 + Psest_3060 0.53 -1.7
3,239,692 + Psest_3060 0.53 -0.1
3,239,693 - Psest_3060 0.54 -1.6
3,239,693 - Psest_3060 0.54 -0.1
3,239,705 - Psest_3060 0.57 -0.9
3,239,790 - Psest_3060 0.81 -0.3
3,239,790 - Psest_3060 0.81 -1.6
3,239,963 + +0.9
3,239,983 + +0.3
3,239,983 + -2.9
3,239,983 + +2.4
3,239,983 + -1.2
3,239,991 + -1.9
3,240,014 + -1.3
3,240,129 + +1.2
3,240,363 + Psest_3061 0.27 -0.8
3,240,364 - Psest_3061 0.27 -1.2
3,240,364 - Psest_3061 0.27 -1.1
3,240,449 - Psest_3061 0.39 -2.4
3,240,459 + Psest_3061 0.40 -0.0
3,240,473 + Psest_3061 0.42 +0.7
3,240,473 + Psest_3061 0.42 -0.1
3,240,506 - Psest_3061 0.46 -1.6
3,240,579 - Psest_3061 0.55 -1.2
3,240,602 + Psest_3061 0.58 -1.0
3,240,602 + Psest_3061 0.58 +0.3
3,240,602 + Psest_3061 0.58 -0.4
3,240,603 - Psest_3061 0.58 -0.1
3,240,603 - Psest_3061 0.58 +0.0
3,240,603 - Psest_3061 0.58 -1.3
3,240,606 + Psest_3061 0.59 -0.2
3,240,606 + Psest_3061 0.59 -1.8
3,240,606 + Psest_3061 0.59 +0.4
3,240,606 + Psest_3061 0.59 +0.1
3,240,606 + Psest_3061 0.59 -0.0
3,240,607 - Psest_3061 0.59 -1.7
3,240,607 - Psest_3061 0.59 -0.0
3,240,607 - Psest_3061 0.59 -2.1
3,240,657 + Psest_3061 0.65 -0.8
3,240,657 + Psest_3061 0.65 +1.2
3,240,658 - Psest_3061 0.66 +0.6
3,240,659 + Psest_3061 0.66 +0.5
3,240,659 + Psest_3061 0.66 +0.3
3,240,659 + Psest_3061 0.66 +0.5
3,240,659 + Psest_3061 0.66 -0.8
3,240,659 + Psest_3061 0.66 -1.2
3,240,659 + Psest_3061 0.66 -0.5
3,240,659 + Psest_3061 0.66 +3.5
3,240,659 + Psest_3061 0.66 +0.7
3,240,659 + Psest_3061 0.66 +0.1
3,240,660 - Psest_3061 0.66 -0.9
3,240,660 - Psest_3061 0.66 -2.0
3,240,660 - Psest_3061 0.66 -3.2
3,240,660 - Psest_3061 0.66 -1.2
3,240,746 + Psest_3061 0.77 -0.5

Or see this region's nucleotide sequence