Experiment: RCH2_defined_1percent_glucose with Chloride 400 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01505 and BWI76_RS01510 are separated by 409 nucleotides BWI76_RS01510 and BWI76_RS01515 are separated by 37 nucleotides BWI76_RS01515 and BWI76_RS01520 are separated by 3 nucleotides BWI76_RS01520 and BWI76_RS01525 are separated by 172 nucleotides
BWI76_RS01505: BWI76_RS01505 - DNA-directed RNA polymerase subunit beta', at 303,893 to 308,116
_RS01505
BWI76_RS01510: BWI76_RS01510 - anaerobic C4-dicarboxylate transporter, at 308,526 to 309,866
_RS01510
BWI76_RS01515: BWI76_RS01515 - PTS family cellobiose transporter subunit IIA, at 309,904 to 310,221
_RS01515
BWI76_RS01520: BWI76_RS01520 - PTS sugar transporter subunit IIB, at 310,225 to 310,530
_RS01520
BWI76_RS01525: BWI76_RS01525 - sensor domain-containing diguanylate cyclase, at 310,703 to 312,235
_RS01525
Position (kb) 308
309
310 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 308.068 kb on + strand at 308.157 kb on - strand at 308.264 kb on - strand at 308.361 kb on - strand at 308.395 kb on + strand at 308.410 kb on + strand at 308.410 kb on + strand at 308.418 kb on + strand at 308.418 kb on - strand at 308.420 kb on + strand at 308.420 kb on + strand at 308.420 kb on + strand at 308.420 kb on + strand at 308.420 kb on + strand at 308.420 kb on + strand at 308.420 kb on + strand at 308.420 kb on + strand at 308.420 kb on + strand at 308.428 kb on - strand at 308.428 kb on - strand at 308.428 kb on - strand at 308.428 kb on - strand at 308.431 kb on + strand at 308.431 kb on + strand at 308.431 kb on + strand at 308.431 kb on + strand at 308.432 kb on + strand at 308.433 kb on - strand at 308.439 kb on - strand at 308.439 kb on - strand at 308.439 kb on - strand at 308.462 kb on - strand at 308.488 kb on + strand at 308.496 kb on - strand at 308.511 kb on + strand at 308.512 kb on + strand at 308.512 kb on + strand at 308.512 kb on + strand at 308.512 kb on + strand at 308.520 kb on - strand at 308.567 kb on + strand at 308.567 kb on + strand at 308.567 kb on + strand at 308.567 kb on + strand at 308.567 kb on + strand at 308.567 kb on + strand at 308.567 kb on + strand at 308.567 kb on + strand at 308.567 kb on + strand at 308.575 kb on - strand at 308.575 kb on - strand at 308.575 kb on - strand at 308.575 kb on - strand at 308.575 kb on - strand at 308.575 kb on - strand at 308.575 kb on - strand at 308.590 kb on + strand at 308.590 kb on + strand at 308.590 kb on + strand at 308.590 kb on + strand at 308.590 kb on + strand at 308.590 kb on + strand at 308.590 kb on + strand at 308.598 kb on - strand at 308.598 kb on - strand at 308.614 kb on + strand at 308.622 kb on - strand at 308.627 kb on - strand at 308.627 kb on - strand at 308.700 kb on - strand, within BWI76_RS01510 at 308.710 kb on + strand, within BWI76_RS01510 at 308.734 kb on + strand, within BWI76_RS01510 at 308.735 kb on + strand, within BWI76_RS01510 at 308.741 kb on - strand, within BWI76_RS01510 at 308.742 kb on - strand, within BWI76_RS01510 at 308.742 kb on - strand, within BWI76_RS01510 at 308.742 kb on - strand, within BWI76_RS01510 at 308.742 kb on - strand, within BWI76_RS01510 at 308.744 kb on + strand, within BWI76_RS01510 at 308.815 kb on + strand, within BWI76_RS01510 at 308.823 kb on - strand, within BWI76_RS01510 at 308.861 kb on - strand, within BWI76_RS01510 at 308.861 kb on - strand, within BWI76_RS01510 at 308.861 kb on - strand, within BWI76_RS01510 at 308.865 kb on - strand, within BWI76_RS01510 at 308.923 kb on - strand, within BWI76_RS01510 at 308.946 kb on - strand, within BWI76_RS01510 at 308.953 kb on - strand, within BWI76_RS01510 at 308.983 kb on + strand, within BWI76_RS01510 at 309.007 kb on + strand, within BWI76_RS01510 at 309.060 kb on + strand, within BWI76_RS01510 at 309.224 kb on - strand, within BWI76_RS01510 at 309.324 kb on - strand, within BWI76_RS01510 at 309.517 kb on - strand, within BWI76_RS01510 at 309.553 kb on - strand, within BWI76_RS01510 at 309.559 kb on - strand, within BWI76_RS01510 at 309.747 kb on + strand at 309.976 kb on + strand, within BWI76_RS01515 at 309.981 kb on + strand, within BWI76_RS01515 at 310.030 kb on + strand, within BWI76_RS01515 at 310.030 kb on + strand, within BWI76_RS01515 at 310.038 kb on - strand, within BWI76_RS01515 at 310.178 kb on + strand, within BWI76_RS01515 at 310.237 kb on + strand at 310.325 kb on + strand, within BWI76_RS01520 at 310.325 kb on + strand, within BWI76_RS01520 at 310.325 kb on + strand, within BWI76_RS01520 at 310.325 kb on + strand, within BWI76_RS01520 at 310.333 kb on - strand, within BWI76_RS01520 at 310.359 kb on + strand, within BWI76_RS01520 at 310.518 kb on - strand at 310.548 kb on - strand at 310.616 kb on - strand at 310.695 kb on - strand at 310.810 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 400 mM and Betaine 100 uM remove 308,068 + +0.4 308,157 - -0.3 308,264 - -0.6 308,361 - -0.0 308,395 + +1.8 308,410 + +0.9 308,410 + -0.0 308,418 + +0.7 308,418 - -0.3 308,420 + -0.2 308,420 + +0.1 308,420 + +0.8 308,420 + +0.4 308,420 + +0.1 308,420 + -2.0 308,420 + +0.3 308,420 + -0.2 308,420 + +0.6 308,428 - +0.5 308,428 - -0.2 308,428 - -1.5 308,428 - +0.4 308,431 + +0.3 308,431 + +0.9 308,431 + +0.2 308,431 + +0.5 308,432 + -0.2 308,433 - -0.0 308,439 - +0.4 308,439 - -2.5 308,439 - -0.3 308,462 - +0.2 308,488 + -1.7 308,496 - -2.9 308,511 + +0.7 308,512 + -0.7 308,512 + +0.4 308,512 + -2.2 308,512 + +0.2 308,520 - +0.3 308,567 + -0.5 308,567 + -1.1 308,567 + -0.2 308,567 + -0.4 308,567 + +0.3 308,567 + -0.0 308,567 + -2.4 308,567 + -0.1 308,567 + -0.6 308,575 - +0.0 308,575 - -0.5 308,575 - +1.9 308,575 - +1.4 308,575 - +0.1 308,575 - +0.3 308,575 - -0.2 308,590 + -0.3 308,590 + +0.6 308,590 + -0.4 308,590 + -1.0 308,590 + +0.0 308,590 + -0.0 308,590 + -0.3 308,598 - -2.2 308,598 - -0.2 308,614 + +0.6 308,622 - -0.8 308,627 - -0.5 308,627 - -0.7 308,700 - BWI76_RS01510 0.13 -3.0 308,710 + BWI76_RS01510 0.14 +0.7 308,734 + BWI76_RS01510 0.16 +0.0 308,735 + BWI76_RS01510 0.16 +2.6 308,741 - BWI76_RS01510 0.16 +1.0 308,742 - BWI76_RS01510 0.16 +0.7 308,742 - BWI76_RS01510 0.16 -0.6 308,742 - BWI76_RS01510 0.16 -0.2 308,742 - BWI76_RS01510 0.16 +1.6 308,744 + BWI76_RS01510 0.16 +0.2 308,815 + BWI76_RS01510 0.22 +0.5 308,823 - BWI76_RS01510 0.22 +0.4 308,861 - BWI76_RS01510 0.25 +0.0 308,861 - BWI76_RS01510 0.25 +0.7 308,861 - BWI76_RS01510 0.25 -0.3 308,865 - BWI76_RS01510 0.25 -0.0 308,923 - BWI76_RS01510 0.30 +0.3 308,946 - BWI76_RS01510 0.31 +0.9 308,953 - BWI76_RS01510 0.32 -0.0 308,983 + BWI76_RS01510 0.34 +1.0 309,007 + BWI76_RS01510 0.36 +0.1 309,060 + BWI76_RS01510 0.40 +0.1 309,224 - BWI76_RS01510 0.52 -0.1 309,324 - BWI76_RS01510 0.60 +1.4 309,517 - BWI76_RS01510 0.74 -0.1 309,553 - BWI76_RS01510 0.77 -0.5 309,559 - BWI76_RS01510 0.77 -2.1 309,747 + -0.0 309,976 + BWI76_RS01515 0.23 +0.7 309,981 + BWI76_RS01515 0.24 -1.2 310,030 + BWI76_RS01515 0.40 -0.3 310,030 + BWI76_RS01515 0.40 +0.6 310,038 - BWI76_RS01515 0.42 -1.7 310,178 + BWI76_RS01515 0.86 +0.1 310,237 + -0.4 310,325 + BWI76_RS01520 0.33 -0.2 310,325 + BWI76_RS01520 0.33 +0.1 310,325 + BWI76_RS01520 0.33 -0.1 310,325 + BWI76_RS01520 0.33 -0.3 310,333 - BWI76_RS01520 0.35 +0.1 310,359 + BWI76_RS01520 0.44 +0.7 310,518 - -0.4 310,548 - +0.2 310,616 - +0.0 310,695 - -0.2 310,810 - -0.5
Or see this region's nucleotide sequence