Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2124

Experiment: syringaldehyde 4 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2123 and Psest_2124 are separated by 51 nucleotidesPsest_2124 and Psest_2125 are separated by 61 nucleotides Psest_2123: Psest_2123 - fusion of gluconokinase (EC 2.7.1.12) and the small permease component of the D-gluconate TRAP transporter (from data), at 2,254,074 to 2,255,204 _2123 Psest_2124: Psest_2124 - D-gluconate TRAP transporter, large permease component (from data), at 2,255,256 to 2,256,533 _2124 Psest_2125: Psest_2125 - D-gluconate TRAP transporter, periplasmic component (from data), at 2,256,595 to 2,257,611 _2125 Position (kb) 2255 2256 2257Strain fitness (log2 ratio) -3 -2 -1 0 1at 2254.535 kb on - strand, within Psest_2123at 2254.664 kb on + strand, within Psest_2123at 2254.665 kb on - strand, within Psest_2123at 2254.698 kb on + strand, within Psest_2123at 2255.228 kb on - strandat 2255.228 kb on - strandat 2255.255 kb on + strandat 2255.256 kb on - strandat 2255.256 kb on - strandat 2255.256 kb on - strandat 2255.256 kb on - strandat 2255.256 kb on - strandat 2255.256 kb on - strandat 2255.508 kb on + strand, within Psest_2124at 2255.509 kb on - strand, within Psest_2124at 2255.509 kb on - strand, within Psest_2124at 2255.509 kb on - strand, within Psest_2124at 2255.730 kb on + strand, within Psest_2124at 2255.730 kb on + strand, within Psest_2124at 2255.730 kb on + strand, within Psest_2124at 2255.731 kb on - strand, within Psest_2124at 2255.994 kb on + strand, within Psest_2124at 2255.994 kb on + strand, within Psest_2124at 2256.123 kb on + strand, within Psest_2124at 2256.123 kb on + strand, within Psest_2124at 2256.123 kb on + strand, within Psest_2124at 2256.123 kb on + strand, within Psest_2124at 2256.123 kb on + strand, within Psest_2124at 2256.123 kb on + strand, within Psest_2124at 2256.124 kb on - strand, within Psest_2124at 2256.458 kb on + strandat 2256.458 kb on + strandat 2256.459 kb on - strandat 2256.462 kb on + strandat 2256.462 kb on + strandat 2256.462 kb on + strandat 2256.462 kb on + strandat 2256.462 kb on + strandat 2256.462 kb on + strandat 2256.463 kb on - strandat 2256.463 kb on - strandat 2256.463 kb on - strandat 2256.463 kb on - strandat 2256.798 kb on + strand, within Psest_2125at 2256.798 kb on + strand, within Psest_2125at 2256.799 kb on - strand, within Psest_2125at 2256.799 kb on - strand, within Psest_2125at 2256.799 kb on - strand, within Psest_2125at 2257.270 kb on + strand, within Psest_2125at 2257.271 kb on - strand, within Psest_2125at 2257.285 kb on + strand, within Psest_2125at 2257.285 kb on + strand, within Psest_2125at 2257.285 kb on + strand, within Psest_2125at 2257.309 kb on + strand, within Psest_2125

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Per-strain Table

Position Strand Gene LocusTag Fraction syringaldehyde 4 mM
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2,254,535 - Psest_2123 0.41 -0.9
2,254,664 + Psest_2123 0.52 -2.8
2,254,665 - Psest_2123 0.52 -2.5
2,254,698 + Psest_2123 0.55 +1.1
2,255,228 - +0.4
2,255,228 - -0.2
2,255,255 + +0.8
2,255,256 - +0.7
2,255,256 - +0.1
2,255,256 - -0.6
2,255,256 - +0.9
2,255,256 - +0.8
2,255,256 - -0.1
2,255,508 + Psest_2124 0.20 +0.2
2,255,509 - Psest_2124 0.20 -0.4
2,255,509 - Psest_2124 0.20 -0.1
2,255,509 - Psest_2124 0.20 -0.2
2,255,730 + Psest_2124 0.37 -0.3
2,255,730 + Psest_2124 0.37 -1.0
2,255,730 + Psest_2124 0.37 +0.6
2,255,731 - Psest_2124 0.37 -2.2
2,255,994 + Psest_2124 0.58 -0.8
2,255,994 + Psest_2124 0.58 -1.1
2,256,123 + Psest_2124 0.68 +0.2
2,256,123 + Psest_2124 0.68 -1.3
2,256,123 + Psest_2124 0.68 -1.2
2,256,123 + Psest_2124 0.68 -0.3
2,256,123 + Psest_2124 0.68 +0.4
2,256,123 + Psest_2124 0.68 -0.9
2,256,124 - Psest_2124 0.68 +0.9
2,256,458 + +0.1
2,256,458 + +0.6
2,256,459 - -0.3
2,256,462 + +0.0
2,256,462 + +0.2
2,256,462 + -0.8
2,256,462 + -0.1
2,256,462 + -0.9
2,256,462 + +0.4
2,256,463 - +1.4
2,256,463 - +0.2
2,256,463 - +0.2
2,256,463 - -1.1
2,256,798 + Psest_2125 0.20 +1.6
2,256,798 + Psest_2125 0.20 +0.5
2,256,799 - Psest_2125 0.20 -1.2
2,256,799 - Psest_2125 0.20 -0.8
2,256,799 - Psest_2125 0.20 -0.7
2,257,270 + Psest_2125 0.66 -1.3
2,257,271 - Psest_2125 0.66 -0.3
2,257,285 + Psest_2125 0.68 -0.2
2,257,285 + Psest_2125 0.68 -0.3
2,257,285 + Psest_2125 0.68 +0.9
2,257,309 + Psest_2125 0.70 -1.1

Or see this region's nucleotide sequence