Experiment: RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09280 and BWI76_RS09285 are separated by 95 nucleotides BWI76_RS09285 and BWI76_RS09290 overlap by 1 nucleotides BWI76_RS09290 and BWI76_RS09295 are separated by 3 nucleotides BWI76_RS09295 and BWI76_RS09300 overlap by 20 nucleotides BWI76_RS09300 and BWI76_RS09305 are separated by 4 nucleotides BWI76_RS09305 and BWI76_RS09310 overlap by 4 nucleotides BWI76_RS09310 and BWI76_RS09315 overlap by 71 nucleotides BWI76_RS09315 and BWI76_RS09320 overlap by 38 nucleotides
BWI76_RS09280: BWI76_RS09280 - terminase endonuclease subunit, at 1,954,962 to 1,955,615
_RS09280
BWI76_RS09285: BWI76_RS09285 - hypothetical protein, at 1,955,711 to 1,956,175
_RS09285
BWI76_RS09290: BWI76_RS09290 - phage Tail Protein X, at 1,956,175 to 1,956,378
_RS09290
BWI76_RS09295: BWI76_RS09295 - hypothetical protein, at 1,956,382 to 1,956,597
_RS09295
BWI76_RS09300: BWI76_RS09300 - phage lysozyme, at 1,956,578 to 1,957,087
_RS09300
BWI76_RS09305: BWI76_RS09305 - hypothetical protein, at 1,957,092 to 1,957,475
_RS09305
BWI76_RS09310: BWI76_RS09310 - hypothetical protein, at 1,957,472 to 1,957,900
_RS09310
BWI76_RS09315: BWI76_RS09315 - hypothetical protein, at 1,957,830 to 1,958,033
_RS09315
BWI76_RS09320: BWI76_RS09320 - P2 phage tail completion protein R, at 1,957,996 to 1,958,427
_RS09320
Position (kb) 1956
1957
1958 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1955.985 kb on - strand, within BWI76_RS09285 at 1955.989 kb on + strand, within BWI76_RS09285 at 1956.256 kb on - strand, within BWI76_RS09290 at 1956.335 kb on - strand, within BWI76_RS09290 at 1956.443 kb on - strand, within BWI76_RS09295 at 1956.467 kb on + strand, within BWI76_RS09295 at 1956.467 kb on + strand, within BWI76_RS09295 at 1956.486 kb on + strand, within BWI76_RS09295 at 1956.656 kb on + strand, within BWI76_RS09300 at 1956.860 kb on + strand, within BWI76_RS09300 at 1956.865 kb on + strand, within BWI76_RS09300 at 1956.866 kb on - strand, within BWI76_RS09300 at 1956.868 kb on - strand, within BWI76_RS09300 at 1956.995 kb on - strand, within BWI76_RS09300 at 1957.127 kb on + strand at 1957.194 kb on + strand, within BWI76_RS09305 at 1957.207 kb on - strand, within BWI76_RS09305 at 1957.282 kb on + strand, within BWI76_RS09305 at 1957.650 kb on - strand, within BWI76_RS09310 at 1957.728 kb on - strand, within BWI76_RS09310 at 1957.922 kb on + strand, within BWI76_RS09315 at 1957.928 kb on + strand, within BWI76_RS09315 at 1958.005 kb on + strand at 1958.055 kb on - strand, within BWI76_RS09320
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM remove 1,955,985 - BWI76_RS09285 0.59 -0.3 1,955,989 + BWI76_RS09285 0.60 +0.2 1,956,256 - BWI76_RS09290 0.40 -0.3 1,956,335 - BWI76_RS09290 0.78 -0.1 1,956,443 - BWI76_RS09295 0.28 -0.7 1,956,467 + BWI76_RS09295 0.39 +0.2 1,956,467 + BWI76_RS09295 0.39 +0.2 1,956,486 + BWI76_RS09295 0.48 +2.4 1,956,656 + BWI76_RS09300 0.15 +0.4 1,956,860 + BWI76_RS09300 0.55 +0.8 1,956,865 + BWI76_RS09300 0.56 +1.7 1,956,866 - BWI76_RS09300 0.56 +0.5 1,956,868 - BWI76_RS09300 0.57 -0.2 1,956,995 - BWI76_RS09300 0.82 +0.3 1,957,127 + -1.2 1,957,194 + BWI76_RS09305 0.27 +0.1 1,957,207 - BWI76_RS09305 0.30 -1.1 1,957,282 + BWI76_RS09305 0.49 +1.0 1,957,650 - BWI76_RS09310 0.41 -0.1 1,957,728 - BWI76_RS09310 0.60 +0.2 1,957,922 + BWI76_RS09315 0.45 +0.5 1,957,928 + BWI76_RS09315 0.48 -2.1 1,958,005 + -0.0 1,958,055 - BWI76_RS09320 0.14 +0.4
Or see this region's nucleotide sequence