Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27405

Experiment: RCH2_defined_1percent_glucose with Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27400 and BWI76_RS27405 are separated by 275 nucleotidesBWI76_RS27405 and BWI76_RS27410 are separated by 5 nucleotides BWI76_RS27400: BWI76_RS27400 - chloride channel protein, at 5,684,883 to 5,686,142 _RS27400 BWI76_RS27405: BWI76_RS27405 - membrane protein, at 5,686,418 to 5,687,035 _RS27405 BWI76_RS27410: BWI76_RS27410 - DNA ligase B, at 5,687,041 to 5,688,717 _RS27410 Position (kb) 5686 5687 5688Strain fitness (log2 ratio) -1 0 1at 5685.446 kb on + strand, within BWI76_RS27400at 5685.587 kb on - strand, within BWI76_RS27400at 5685.619 kb on + strand, within BWI76_RS27400at 5685.683 kb on + strand, within BWI76_RS27400at 5685.773 kb on - strand, within BWI76_RS27400at 5685.784 kb on - strand, within BWI76_RS27400at 5685.797 kb on - strand, within BWI76_RS27400at 5685.843 kb on - strand, within BWI76_RS27400at 5685.890 kb on + strand, within BWI76_RS27400at 5685.954 kb on - strand, within BWI76_RS27400at 5685.967 kb on - strand, within BWI76_RS27400at 5685.998 kb on + strand, within BWI76_RS27400at 5686.006 kb on - strand, within BWI76_RS27400at 5686.152 kb on + strandat 5686.224 kb on + strandat 5686.224 kb on + strandat 5686.231 kb on - strandat 5686.231 kb on - strandat 5686.232 kb on - strandat 5686.421 kb on - strandat 5686.421 kb on - strandat 5686.460 kb on + strandat 5686.545 kb on - strand, within BWI76_RS27405at 5686.590 kb on + strand, within BWI76_RS27405at 5686.592 kb on + strand, within BWI76_RS27405at 5686.598 kb on - strand, within BWI76_RS27405at 5686.634 kb on + strand, within BWI76_RS27405at 5686.784 kb on + strand, within BWI76_RS27405at 5686.816 kb on - strand, within BWI76_RS27405at 5686.846 kb on - strand, within BWI76_RS27405at 5686.864 kb on - strand, within BWI76_RS27405at 5686.899 kb on - strand, within BWI76_RS27405at 5686.899 kb on - strandat 5686.986 kb on + strandat 5687.283 kb on + strand, within BWI76_RS27410at 5687.316 kb on + strand, within BWI76_RS27410at 5687.413 kb on - strand, within BWI76_RS27410at 5687.515 kb on + strand, within BWI76_RS27410at 5687.515 kb on + strand, within BWI76_RS27410at 5687.557 kb on + strand, within BWI76_RS27410at 5687.557 kb on + strand, within BWI76_RS27410at 5687.580 kb on - strand, within BWI76_RS27410at 5687.757 kb on + strand, within BWI76_RS27410at 5687.786 kb on - strand, within BWI76_RS27410at 5687.941 kb on - strand, within BWI76_RS27410at 5688.026 kb on - strand, within BWI76_RS27410

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Betaine 100 uM
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5,685,446 + BWI76_RS27400 0.45 -0.1
5,685,587 - BWI76_RS27400 0.56 +0.0
5,685,619 + BWI76_RS27400 0.58 +0.3
5,685,683 + BWI76_RS27400 0.63 +0.3
5,685,773 - BWI76_RS27400 0.71 +0.1
5,685,784 - BWI76_RS27400 0.72 +0.0
5,685,797 - BWI76_RS27400 0.73 +1.4
5,685,843 - BWI76_RS27400 0.76 -0.9
5,685,890 + BWI76_RS27400 0.80 -0.0
5,685,954 - BWI76_RS27400 0.85 +0.1
5,685,967 - BWI76_RS27400 0.86 +0.5
5,685,998 + BWI76_RS27400 0.88 +0.4
5,686,006 - BWI76_RS27400 0.89 +0.6
5,686,152 + +0.4
5,686,224 + +0.2
5,686,224 + -0.1
5,686,231 - +0.2
5,686,231 - +0.1
5,686,232 - -1.4
5,686,421 - +0.0
5,686,421 - -0.0
5,686,460 + +1.4
5,686,545 - BWI76_RS27405 0.21 +0.3
5,686,590 + BWI76_RS27405 0.28 +0.3
5,686,592 + BWI76_RS27405 0.28 +1.2
5,686,598 - BWI76_RS27405 0.29 +0.1
5,686,634 + BWI76_RS27405 0.35 +1.6
5,686,784 + BWI76_RS27405 0.59 +1.3
5,686,816 - BWI76_RS27405 0.64 +0.7
5,686,846 - BWI76_RS27405 0.69 -0.0
5,686,864 - BWI76_RS27405 0.72 +0.1
5,686,899 - BWI76_RS27405 0.78 +1.2
5,686,899 - +1.6
5,686,986 + -0.7
5,687,283 + BWI76_RS27410 0.14 -0.4
5,687,316 + BWI76_RS27410 0.16 -0.2
5,687,413 - BWI76_RS27410 0.22 -0.2
5,687,515 + BWI76_RS27410 0.28 +0.1
5,687,515 + BWI76_RS27410 0.28 +0.4
5,687,557 + BWI76_RS27410 0.31 +0.5
5,687,557 + BWI76_RS27410 0.31 -0.2
5,687,580 - BWI76_RS27410 0.32 -0.0
5,687,757 + BWI76_RS27410 0.43 -0.7
5,687,786 - BWI76_RS27410 0.44 -0.1
5,687,941 - BWI76_RS27410 0.54 -0.2
5,688,026 - BWI76_RS27410 0.59 +0.5

Or see this region's nucleotide sequence