Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03605

Experiment: RCH2_defined_1percent_glucose with Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03590 and BWI76_RS03595 are separated by 53 nucleotidesBWI76_RS03595 and BWI76_RS03600 are separated by 15 nucleotidesBWI76_RS03600 and BWI76_RS03605 are separated by 17 nucleotidesBWI76_RS03605 and BWI76_RS03610 are separated by 406 nucleotidesBWI76_RS03610 and BWI76_RS03615 overlap by 4 nucleotides BWI76_RS03590: BWI76_RS03590 - acetaldehyde dehydrogenase (acetylating), at 744,308 to 745,909 _RS03590 BWI76_RS03595: BWI76_RS03595 - ethanolamine utilization protein EutM, at 745,963 to 746,241 _RS03595 BWI76_RS03600: BWI76_RS03600 - BMC domain-containing protein, at 746,257 to 746,541 _RS03600 BWI76_RS03605: BWI76_RS03605 - BMC domain-containing protein, at 746,559 to 746,837 _RS03605 BWI76_RS03610: BWI76_RS03610 - hypothetical protein, at 747,244 to 747,762 _RS03610 BWI76_RS03615: BWI76_RS03615 - putative transcriptional regulatory protein, at 747,759 to 748,559 _RS03615 Position (kb) 746 747Strain fitness (log2 ratio) -2 -1 0 1at 745.869 kb on - strandat 745.883 kb on + strandat 746.034 kb on - strand, within BWI76_RS03595at 746.173 kb on - strand, within BWI76_RS03595at 746.505 kb on - strand, within BWI76_RS03600at 746.523 kb on + strandat 746.557 kb on - strandat 746.557 kb on - strandat 746.695 kb on + strand, within BWI76_RS03605at 746.725 kb on - strand, within BWI76_RS03605at 746.769 kb on - strand, within BWI76_RS03605at 746.819 kb on + strandat 746.819 kb on + strandat 746.827 kb on - strandat 746.827 kb on - strandat 747.301 kb on + strand, within BWI76_RS03610at 747.338 kb on + strand, within BWI76_RS03610at 747.652 kb on + strand, within BWI76_RS03610at 747.722 kb on + strandat 747.809 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Betaine 100 uM
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745,869 - +0.2
745,883 + -0.6
746,034 - BWI76_RS03595 0.25 -2.2
746,173 - BWI76_RS03595 0.75 -1.2
746,505 - BWI76_RS03600 0.87 +0.1
746,523 + -0.4
746,557 - +1.2
746,557 - -0.0
746,695 + BWI76_RS03605 0.49 +0.2
746,725 - BWI76_RS03605 0.59 -0.4
746,769 - BWI76_RS03605 0.75 -0.3
746,819 + -1.9
746,819 + +1.1
746,827 - +0.1
746,827 - +0.0
747,301 + BWI76_RS03610 0.11 +0.8
747,338 + BWI76_RS03610 0.18 -0.1
747,652 + BWI76_RS03610 0.79 +0.0
747,722 + +0.7
747,809 + +1.6

Or see this region's nucleotide sequence