Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27255

Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27250 and BWI76_RS27255 are separated by 9 nucleotidesBWI76_RS27255 and BWI76_RS27260 are separated by 214 nucleotides BWI76_RS27250: BWI76_RS27250 - L-threonine 3-dehydrogenase, at 5,655,478 to 5,656,503 _RS27250 BWI76_RS27255: BWI76_RS27255 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (from data), at 5,656,513 to 5,657,706 _RS27255 BWI76_RS27260: BWI76_RS27260 - ADP-L-glycero-D-mannoheptose-6-epimerase, at 5,657,921 to 5,658,853 _RS27260 Position (kb) 5656 5657 5658Strain fitness (log2 ratio) -2 -1 0 1 2at 5655.525 kb on + strandat 5655.574 kb on - strandat 5655.605 kb on - strand, within BWI76_RS27250at 5655.798 kb on - strand, within BWI76_RS27250at 5655.798 kb on - strand, within BWI76_RS27250at 5655.815 kb on + strandat 5655.823 kb on - strand, within BWI76_RS27250at 5655.839 kb on + strand, within BWI76_RS27250at 5655.887 kb on - strand, within BWI76_RS27250at 5655.987 kb on - strand, within BWI76_RS27250at 5655.987 kb on - strand, within BWI76_RS27250at 5656.263 kb on + strand, within BWI76_RS27250at 5656.271 kb on - strand, within BWI76_RS27250at 5656.278 kb on + strand, within BWI76_RS27250at 5656.278 kb on + strand, within BWI76_RS27250at 5656.286 kb on - strand, within BWI76_RS27250at 5656.311 kb on + strand, within BWI76_RS27250at 5656.364 kb on - strand, within BWI76_RS27250at 5656.388 kb on - strand, within BWI76_RS27250at 5656.465 kb on + strandat 5656.510 kb on + strandat 5656.525 kb on + strandat 5656.545 kb on + strandat 5656.546 kb on + strandat 5656.560 kb on - strandat 5656.700 kb on + strand, within BWI76_RS27255at 5656.718 kb on - strand, within BWI76_RS27255at 5656.783 kb on + strand, within BWI76_RS27255at 5657.029 kb on - strand, within BWI76_RS27255at 5657.029 kb on - strand, within BWI76_RS27255at 5657.134 kb on - strand, within BWI76_RS27255at 5657.137 kb on + strand, within BWI76_RS27255at 5657.153 kb on + strand, within BWI76_RS27255at 5657.275 kb on - strand, within BWI76_RS27255at 5657.277 kb on + strand, within BWI76_RS27255at 5657.305 kb on + strand, within BWI76_RS27255at 5657.313 kb on - strand, within BWI76_RS27255at 5657.313 kb on - strand, within BWI76_RS27255at 5657.394 kb on - strand, within BWI76_RS27255at 5657.447 kb on - strandat 5657.529 kb on + strand, within BWI76_RS27255at 5657.629 kb on - strandat 5657.663 kb on + strandat 5657.663 kb on + strandat 5657.671 kb on - strandat 5657.671 kb on - strandat 5657.673 kb on - strandat 5657.751 kb on + strandat 5657.758 kb on + strandat 5657.759 kb on - strandat 5657.759 kb on - strandat 5657.759 kb on - strandat 5657.759 kb on - strandat 5657.814 kb on + strandat 5657.815 kb on + strandat 5657.815 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM
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5,655,525 + +0.7
5,655,574 - +1.8
5,655,605 - BWI76_RS27250 0.12 +0.6
5,655,798 - BWI76_RS27250 0.31 +1.0
5,655,798 - BWI76_RS27250 0.31 +1.4
5,655,815 + +1.2
5,655,823 - BWI76_RS27250 0.34 +1.1
5,655,839 + BWI76_RS27250 0.35 +1.0
5,655,887 - BWI76_RS27250 0.40 +1.1
5,655,987 - BWI76_RS27250 0.50 +1.3
5,655,987 - BWI76_RS27250 0.50 +0.2
5,656,263 + BWI76_RS27250 0.77 +1.5
5,656,271 - BWI76_RS27250 0.77 +1.8
5,656,278 + BWI76_RS27250 0.78 -1.2
5,656,278 + BWI76_RS27250 0.78 +2.3
5,656,286 - BWI76_RS27250 0.79 +0.9
5,656,311 + BWI76_RS27250 0.81 +1.2
5,656,364 - BWI76_RS27250 0.86 +1.0
5,656,388 - BWI76_RS27250 0.89 +2.1
5,656,465 + +1.1
5,656,510 + +0.9
5,656,525 + +1.1
5,656,545 + +0.9
5,656,546 + +1.3
5,656,560 - -1.8
5,656,700 + BWI76_RS27255 0.16 +1.3
5,656,718 - BWI76_RS27255 0.17 +1.6
5,656,783 + BWI76_RS27255 0.23 -0.7
5,657,029 - BWI76_RS27255 0.43 +0.8
5,657,029 - BWI76_RS27255 0.43 +1.9
5,657,134 - BWI76_RS27255 0.52 +1.0
5,657,137 + BWI76_RS27255 0.52 +1.2
5,657,153 + BWI76_RS27255 0.54 +2.2
5,657,275 - BWI76_RS27255 0.64 +1.1
5,657,277 + BWI76_RS27255 0.64 -2.7
5,657,305 + BWI76_RS27255 0.66 +1.8
5,657,313 - BWI76_RS27255 0.67 +1.7
5,657,313 - BWI76_RS27255 0.67 +1.0
5,657,394 - BWI76_RS27255 0.74 +1.3
5,657,447 - +0.8
5,657,529 + BWI76_RS27255 0.85 +1.2
5,657,629 - -0.8
5,657,663 + +1.1
5,657,663 + +1.2
5,657,671 - +1.8
5,657,671 - +1.5
5,657,673 - +1.8
5,657,751 + +1.2
5,657,758 + +1.0
5,657,759 - +0.6
5,657,759 - -0.1
5,657,759 - -1.5
5,657,759 - -0.7
5,657,814 + +0.6
5,657,815 + +0.6
5,657,815 + -0.7

Or see this region's nucleotide sequence