Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21145 and BWI76_RS21150 are separated by 73 nucleotides BWI76_RS21150 and BWI76_RS21155 are separated by 19 nucleotides BWI76_RS21155 and BWI76_RS21160 are separated by 26 nucleotides BWI76_RS21160 and BWI76_RS21165 are separated by 177 nucleotides BWI76_RS21165 and BWI76_RS21170 are separated by 233 nucleotides
BWI76_RS21145: BWI76_RS21145 - co-chaperone HscB, at 4,428,711 to 4,429,226
_RS21145
BWI76_RS21150: BWI76_RS21150 - iron-sulfur cluster assembly accessory protein, at 4,429,300 to 4,429,623
_RS21150
BWI76_RS21155: BWI76_RS21155 - iron-sulfur cluster scaffold-like protein, at 4,429,643 to 4,430,029
_RS21155
BWI76_RS21160: BWI76_RS21160 - IscS subfamily cysteine desulfurase, at 4,430,056 to 4,431,270
_RS21160
BWI76_RS21165: BWI76_RS21165 - Fe-S cluster assembly transcriptional regulator IscR, at 4,431,448 to 4,431,939
_RS21165
BWI76_RS21170: BWI76_RS21170 - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 4,432,173 to 4,432,904
_RS21170
Position (kb) 4430
4431
4432 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4429.252 kb on + strand at 4429.284 kb on + strand at 4429.713 kb on - strand, within BWI76_RS21155 at 4431.572 kb on - strand, within BWI76_RS21165 at 4432.156 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM remove 4,429,252 + -0.6 4,429,284 + +2.1 4,429,713 - BWI76_RS21155 0.18 -0.1 4,431,572 - BWI76_RS21165 0.25 -1.9 4,432,156 + +1.9
Or see this region's nucleotide sequence