Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14160 and BWI76_RS14165 are separated by 243 nucleotides BWI76_RS14165 and BWI76_RS14170 are separated by 48 nucleotides BWI76_RS14170 and BWI76_RS14175 are separated by 135 nucleotides
BWI76_RS14160: BWI76_RS14160 - ABC transporter substrate-binding protein, at 2,962,550 to 2,963,512
_RS14160
BWI76_RS14165: BWI76_RS14165 - trans-acting regulatory protein of aco operon, at 2,963,756 to 2,966,509
_RS14165
BWI76_RS14170: BWI76_RS14170 - hypothetical protein, at 2,966,558 to 2,966,941
_RS14170
BWI76_RS14175: BWI76_RS14175 - dehydrogenase, at 2,967,077 to 2,967,952
_RS14175
Position (kb)
2963
2964
2965
2966
2967 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2962.964 kb on + strand, within BWI76_RS14160 at 2963.020 kb on - strand, within BWI76_RS14160 at 2963.198 kb on - strand, within BWI76_RS14160 at 2963.241 kb on + strand, within BWI76_RS14160 at 2963.375 kb on + strand, within BWI76_RS14160 at 2963.388 kb on + strand, within BWI76_RS14160 at 2963.481 kb on + strand at 2963.481 kb on + strand at 2963.557 kb on + strand at 2963.826 kb on - strand at 2964.005 kb on - strand at 2964.137 kb on - strand, within BWI76_RS14165 at 2964.246 kb on - strand, within BWI76_RS14165 at 2964.290 kb on - strand, within BWI76_RS14165 at 2964.461 kb on - strand, within BWI76_RS14165 at 2964.696 kb on - strand, within BWI76_RS14165 at 2964.803 kb on - strand, within BWI76_RS14165 at 2965.341 kb on + strand, within BWI76_RS14165 at 2965.341 kb on + strand, within BWI76_RS14165 at 2965.530 kb on - strand at 2965.808 kb on + strand, within BWI76_RS14165 at 2965.813 kb on + strand, within BWI76_RS14165 at 2966.029 kb on + strand, within BWI76_RS14165 at 2966.029 kb on - strand, within BWI76_RS14165 at 2966.341 kb on + strand at 2966.408 kb on - strand at 2966.446 kb on + strand at 2966.561 kb on + strand at 2966.569 kb on - strand at 2966.579 kb on + strand at 2966.656 kb on + strand, within BWI76_RS14170 at 2966.741 kb on - strand, within BWI76_RS14170 at 2966.767 kb on - strand, within BWI76_RS14170 at 2966.840 kb on + strand, within BWI76_RS14170 at 2966.889 kb on - strand, within BWI76_RS14170 at 2966.907 kb on + strand at 2966.979 kb on + strand at 2966.979 kb on + strand at 2967.034 kb on - strand at 2967.036 kb on + strand at 2967.075 kb on + strand at 2967.075 kb on + strand at 2967.123 kb on + strand at 2967.147 kb on - strand at 2967.156 kb on + strand at 2967.220 kb on - strand, within BWI76_RS14175 at 2967.277 kb on + strand at 2967.285 kb on - strand, within BWI76_RS14175 at 2967.335 kb on - strand, within BWI76_RS14175
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM remove 2,962,964 + BWI76_RS14160 0.43 -0.4 2,963,020 - BWI76_RS14160 0.49 -0.2 2,963,198 - BWI76_RS14160 0.67 +0.1 2,963,241 + BWI76_RS14160 0.72 -0.9 2,963,375 + BWI76_RS14160 0.86 +0.1 2,963,388 + BWI76_RS14160 0.87 -0.9 2,963,481 + -0.5 2,963,481 + -2.0 2,963,557 + +0.8 2,963,826 - -2.0 2,964,005 - -0.2 2,964,137 - BWI76_RS14165 0.14 +1.3 2,964,246 - BWI76_RS14165 0.18 +0.4 2,964,290 - BWI76_RS14165 0.19 +1.3 2,964,461 - BWI76_RS14165 0.26 -0.2 2,964,696 - BWI76_RS14165 0.34 +0.2 2,964,803 - BWI76_RS14165 0.38 -2.5 2,965,341 + BWI76_RS14165 0.58 -2.9 2,965,341 + BWI76_RS14165 0.58 +1.3 2,965,530 - +0.7 2,965,808 + BWI76_RS14165 0.75 -0.3 2,965,813 + BWI76_RS14165 0.75 -0.7 2,966,029 + BWI76_RS14165 0.83 +0.1 2,966,029 - BWI76_RS14165 0.83 +1.4 2,966,341 + +0.2 2,966,408 - +0.5 2,966,446 + +0.1 2,966,561 + -1.0 2,966,569 - -0.5 2,966,579 + -2.8 2,966,656 + BWI76_RS14170 0.26 -1.1 2,966,741 - BWI76_RS14170 0.48 +2.2 2,966,767 - BWI76_RS14170 0.54 +2.4 2,966,840 + BWI76_RS14170 0.73 -2.1 2,966,889 - BWI76_RS14170 0.86 +1.5 2,966,907 + +1.7 2,966,979 + +0.2 2,966,979 + -0.3 2,967,034 - -0.6 2,967,036 + +0.3 2,967,075 + +0.6 2,967,075 + +0.2 2,967,123 + -1.1 2,967,147 - -1.9 2,967,156 + +0.4 2,967,220 - BWI76_RS14175 0.16 +0.5 2,967,277 + -0.3 2,967,285 - BWI76_RS14175 0.24 -0.3 2,967,335 - BWI76_RS14175 0.29 -1.1
Or see this region's nucleotide sequence