Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02095 and BWI76_RS02100 are separated by 92 nucleotides BWI76_RS02100 and BWI76_RS02105 are separated by 67 nucleotides BWI76_RS02105 and BWI76_RS02110 are separated by 36 nucleotides BWI76_RS02110 and BWI76_RS02115 are separated by 203 nucleotides
BWI76_RS02095: BWI76_RS02095 - acetyl-coenzyme A synthetase, at 431,834 to 433,792
_RS02095
BWI76_RS02100: BWI76_RS02100 - hypothetical protein, at 433,885 to 434,136
_RS02100
BWI76_RS02105: BWI76_RS02105 - glutamate/aspartate:proton symporter GltP, at 434,204 to 435,517
_RS02105
BWI76_RS02110: BWI76_RS02110 - hypothetical protein, at 435,554 to 436,237
_RS02110
BWI76_RS02115: BWI76_RS02115 - formate dehydrogenase subunit alpha, at 436,441 to 438,588
_RS02115
Position (kb) 434
435
436 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 433.413 kb on + strand, within BWI76_RS02095 at 433.513 kb on - strand, within BWI76_RS02095 at 433.716 kb on + strand at 433.726 kb on + strand at 434.147 kb on + strand at 434.196 kb on + strand at 434.258 kb on - strand at 434.275 kb on - strand at 434.316 kb on + strand at 434.353 kb on - strand, within BWI76_RS02105 at 434.387 kb on + strand, within BWI76_RS02105 at 434.481 kb on + strand, within BWI76_RS02105 at 434.484 kb on - strand, within BWI76_RS02105 at 434.502 kb on + strand, within BWI76_RS02105 at 434.510 kb on - strand, within BWI76_RS02105 at 434.600 kb on + strand, within BWI76_RS02105 at 434.608 kb on - strand, within BWI76_RS02105 at 434.848 kb on + strand, within BWI76_RS02105 at 434.848 kb on + strand, within BWI76_RS02105 at 434.948 kb on + strand, within BWI76_RS02105 at 435.076 kb on + strand, within BWI76_RS02105 at 435.168 kb on - strand, within BWI76_RS02105 at 435.193 kb on + strand, within BWI76_RS02105 at 435.193 kb on + strand, within BWI76_RS02105 at 435.201 kb on - strand, within BWI76_RS02105 at 435.397 kb on - strand at 435.480 kb on + strand at 435.553 kb on - strand at 435.572 kb on + strand at 435.612 kb on - strand at 435.786 kb on + strand, within BWI76_RS02110 at 435.814 kb on - strand, within BWI76_RS02110 at 435.864 kb on - strand, within BWI76_RS02110 at 435.879 kb on + strand, within BWI76_RS02110 at 436.084 kb on - strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.130 kb on - strand, within BWI76_RS02110 at 436.130 kb on - strand, within BWI76_RS02110 at 436.130 kb on - strand, within BWI76_RS02110 at 436.267 kb on - strand at 436.269 kb on + strand at 436.269 kb on + strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.278 kb on - strand at 436.287 kb on - strand at 436.288 kb on - strand at 436.421 kb on + strand at 436.442 kb on - strand at 436.493 kb on + strand at 436.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM remove 433,413 + BWI76_RS02095 0.81 -2.3 433,513 - BWI76_RS02095 0.86 -0.7 433,716 + -0.9 433,726 + -1.0 434,147 + +1.3 434,196 + +1.3 434,258 - +2.4 434,275 - +1.9 434,316 + +1.9 434,353 - BWI76_RS02105 0.11 +1.8 434,387 + BWI76_RS02105 0.14 +1.6 434,481 + BWI76_RS02105 0.21 +1.8 434,484 - BWI76_RS02105 0.21 +2.0 434,502 + BWI76_RS02105 0.23 +1.8 434,510 - BWI76_RS02105 0.23 +0.1 434,600 + BWI76_RS02105 0.30 +4.0 434,608 - BWI76_RS02105 0.31 +0.8 434,848 + BWI76_RS02105 0.49 +1.0 434,848 + BWI76_RS02105 0.49 +2.5 434,948 + BWI76_RS02105 0.57 +1.9 435,076 + BWI76_RS02105 0.66 +2.0 435,168 - BWI76_RS02105 0.73 +1.8 435,193 + BWI76_RS02105 0.75 +2.5 435,193 + BWI76_RS02105 0.75 +1.7 435,201 - BWI76_RS02105 0.76 +2.6 435,397 - +1.5 435,480 + +1.8 435,553 - +0.5 435,572 + -0.1 435,612 - +0.6 435,786 + BWI76_RS02110 0.34 +0.6 435,814 - BWI76_RS02110 0.38 +0.5 435,864 - BWI76_RS02110 0.45 +1.9 435,879 + BWI76_RS02110 0.48 -0.7 436,084 - BWI76_RS02110 0.77 +0.3 436,122 + BWI76_RS02110 0.83 -0.3 436,122 + BWI76_RS02110 0.83 -0.7 436,122 + BWI76_RS02110 0.83 -0.1 436,122 + BWI76_RS02110 0.83 -0.9 436,122 + BWI76_RS02110 0.83 +0.5 436,122 + BWI76_RS02110 0.83 -0.1 436,130 - BWI76_RS02110 0.84 +0.3 436,130 - BWI76_RS02110 0.84 -2.1 436,130 - BWI76_RS02110 0.84 +0.0 436,267 - +0.8 436,269 + +0.3 436,269 + -1.1 436,277 - +1.1 436,277 - +0.6 436,277 - +0.1 436,277 - +0.2 436,277 - -1.3 436,277 - +0.3 436,277 - +0.4 436,278 - +0.7 436,287 - +0.3 436,288 - +1.4 436,421 + +1.3 436,442 - -0.5 436,493 + +0.1 436,501 + -0.4
Or see this region's nucleotide sequence