Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0519 and Psest_0520 are separated by 202 nucleotides Psest_0520 and Psest_0521 are separated by 86 nucleotides Psest_0521 and Psest_0522 are separated by 62 nucleotides Psest_0522 and Psest_0523 are separated by 93 nucleotides
Psest_0519: Psest_0519 - lipoate synthase, at 572,004 to 572,981
_0519
Psest_0520: Psest_0520 - hypothetical protein, at 573,184 to 573,528
_0520
Psest_0521: Psest_0521 - lytic murein transglycosylase, at 573,615 to 574,913
_0521
Psest_0522: Psest_0522 - Uncharacterized protein conserved in bacteria, at 574,976 to 575,134
_0522
Psest_0523: Psest_0523 - DNA polymerase III, delta subunit, at 575,228 to 576,256
_0523
Position (kb) 573
574
575 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 573.125 kb on - strand at 573.125 kb on - strand at 573.136 kb on + strand at 573.136 kb on + strand at 573.136 kb on + strand at 573.136 kb on + strand at 573.136 kb on + strand at 573.136 kb on + strand at 573.137 kb on - strand at 573.138 kb on + strand at 573.153 kb on - strand at 573.153 kb on - strand at 573.200 kb on + strand at 573.382 kb on + strand, within Psest_0520 at 573.500 kb on - strand at 573.500 kb on - strand at 573.505 kb on + strand at 573.505 kb on + strand at 573.506 kb on - strand at 573.552 kb on + strand at 573.552 kb on + strand at 573.553 kb on - strand at 573.886 kb on + strand, within Psest_0521 at 573.886 kb on + strand, within Psest_0521 at 573.904 kb on + strand, within Psest_0521 at 573.904 kb on + strand, within Psest_0521 at 573.926 kb on + strand, within Psest_0521 at 573.927 kb on - strand, within Psest_0521 at 574.144 kb on + strand, within Psest_0521 at 574.144 kb on + strand, within Psest_0521 at 574.145 kb on - strand, within Psest_0521 at 574.145 kb on - strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.571 kb on - strand, within Psest_0521 at 574.677 kb on - strand, within Psest_0521 at 574.677 kb on - strand, within Psest_0521 at 574.772 kb on + strand, within Psest_0521 at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.951 kb on - strand at 574.951 kb on - strand at 574.951 kb on - strand at 575.177 kb on + strand at 575.178 kb on - strand at 575.178 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 573,125 - +1.0 573,125 - +0.3 573,136 + +0.1 573,136 + +0.4 573,136 + -0.1 573,136 + +0.0 573,136 + -0.9 573,136 + +1.2 573,137 - +0.8 573,138 + -0.1 573,153 - +0.1 573,153 - +0.1 573,200 + -1.1 573,382 + Psest_0520 0.57 +0.4 573,500 - +2.2 573,500 - -0.5 573,505 + +0.6 573,505 + -0.2 573,506 - +0.5 573,552 + -2.1 573,552 + +0.5 573,553 - +0.5 573,886 + Psest_0521 0.21 +1.5 573,886 + Psest_0521 0.21 -1.0 573,904 + Psest_0521 0.22 -1.1 573,904 + Psest_0521 0.22 -0.9 573,926 + Psest_0521 0.24 -2.6 573,927 - Psest_0521 0.24 +0.1 574,144 + Psest_0521 0.41 -0.5 574,144 + Psest_0521 0.41 -1.7 574,145 - Psest_0521 0.41 -2.9 574,145 - Psest_0521 0.41 -3.1 574,231 + Psest_0521 0.47 +0.4 574,231 + Psest_0521 0.47 -0.5 574,231 + Psest_0521 0.47 -2.1 574,231 + Psest_0521 0.47 -0.4 574,231 + Psest_0521 0.47 -1.2 574,231 + Psest_0521 0.47 -2.3 574,231 + Psest_0521 0.47 -1.1 574,231 + Psest_0521 0.47 -2.7 574,231 + Psest_0521 0.47 -1.4 574,231 + Psest_0521 0.47 -2.3 574,231 + Psest_0521 0.47 -1.9 574,231 + Psest_0521 0.47 -1.0 574,231 + Psest_0521 0.47 -1.7 574,231 + Psest_0521 0.47 -1.3 574,231 + Psest_0521 0.47 -0.9 574,232 - Psest_0521 0.47 -0.4 574,232 - Psest_0521 0.47 -1.2 574,232 - Psest_0521 0.47 -3.1 574,232 - Psest_0521 0.47 -0.5 574,232 - Psest_0521 0.47 -1.5 574,232 - Psest_0521 0.47 -2.8 574,232 - Psest_0521 0.47 -2.2 574,232 - Psest_0521 0.47 -2.6 574,232 - Psest_0521 0.47 -1.8 574,232 - Psest_0521 0.47 -2.2 574,232 - Psest_0521 0.47 -1.5 574,232 - Psest_0521 0.47 -0.1 574,232 - Psest_0521 0.47 -3.2 574,232 - Psest_0521 0.47 -2.1 574,571 - Psest_0521 0.74 -0.4 574,677 - Psest_0521 0.82 -2.3 574,677 - Psest_0521 0.82 +0.5 574,772 + Psest_0521 0.89 -0.9 574,909 + -0.2 574,909 + -1.2 574,909 + -2.3 574,909 + -1.3 574,909 + -1.4 574,909 + +0.1 574,909 + -3.8 574,909 + -2.9 574,909 + -0.1 574,909 + -0.4 574,910 - -0.9 574,910 - -1.1 574,910 - -1.0 574,910 - -2.4 574,910 - +0.6 574,910 - -1.9 574,951 - -1.8 574,951 - +0.1 574,951 - +0.4 575,177 + -0.7 575,178 - -1.1 575,178 - -1.7
Or see this region's nucleotide sequence