Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0155 and Psest_0156 are separated by 98 nucleotides Psest_0156 and Psest_0157 are separated by 86 nucleotides Psest_0157 and Psest_0158 are separated by 230 nucleotides Psest_0158 and Psest_0159 are separated by 3 nucleotides
Psest_0155: Psest_0155 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 153,628 to 154,371
_0155
Psest_0156: Psest_0156 - imidazoleglycerol phosphate synthase, cyclase subunit, at 154,470 to 155,240
_0156
Psest_0157: Psest_0157 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 155,327 to 156,076
_0157
Psest_0158: Psest_0158 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 156,307 to 157,053
_0158
Psest_0159: Psest_0159 - Uncharacterized protein conserved in bacteria, at 157,057 to 157,848
_0159
Position (kb)
155
156
157 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 154.357 kb on + strand at 154.357 kb on + strand at 154.357 kb on + strand at 154.357 kb on + strand at 154.357 kb on + strand at 154.358 kb on - strand at 154.412 kb on + strand at 154.412 kb on + strand at 154.412 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.670 kb on - strand, within Psest_0156 at 155.184 kb on + strand at 155.184 kb on + strand at 155.184 kb on + strand at 155.185 kb on - strand at 155.185 kb on - strand at 155.185 kb on - strand at 155.185 kb on - strand at 155.208 kb on + strand at 155.209 kb on - strand at 155.266 kb on + strand at 155.267 kb on - strand at 155.267 kb on - strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.368 kb on - strand at 155.438 kb on + strand, within Psest_0157 at 155.439 kb on - strand, within Psest_0157 at 155.540 kb on + strand, within Psest_0157 at 155.540 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.577 kb on - strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.604 kb on - strand, within Psest_0157 at 155.604 kb on - strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.759 kb on + strand, within Psest_0157 at 155.759 kb on + strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.951 kb on - strand, within Psest_0157 at 155.963 kb on + strand, within Psest_0157 at 155.964 kb on - strand, within Psest_0157 at 156.069 kb on - strand at 156.069 kb on - strand at 156.323 kb on + strand at 156.344 kb on + strand at 156.345 kb on - strand at 156.345 kb on - strand at 156.345 kb on - strand at 156.442 kb on + strand, within Psest_0158 at 156.443 kb on - strand, within Psest_0158 at 156.443 kb on - strand, within Psest_0158 at 156.443 kb on - strand, within Psest_0158 at 156.443 kb on - strand, within Psest_0158 at 156.553 kb on + strand, within Psest_0158 at 156.554 kb on - strand, within Psest_0158 at 156.554 kb on - strand, within Psest_0158 at 156.554 kb on - strand, within Psest_0158 at 156.652 kb on - strand, within Psest_0158 at 156.736 kb on + strand, within Psest_0158 at 156.736 kb on + strand, within Psest_0158 at 156.874 kb on + strand, within Psest_0158 at 156.987 kb on - strand at 157.059 kb on - strand at 157.065 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 154,357 + -0.1 154,357 + +0.1 154,357 + -1.4 154,357 + -1.0 154,357 + -0.5 154,358 - +0.5 154,412 + +0.5 154,412 + -0.4 154,412 + -0.4 154,419 + -1.5 154,419 + -0.8 154,419 + -0.8 154,419 + -0.7 154,419 + +1.8 154,420 - +0.8 154,420 - +0.2 154,420 - -1.0 154,420 - +2.5 154,420 - -1.1 154,670 - Psest_0156 0.26 +0.7 155,184 + -0.0 155,184 + -0.6 155,184 + +0.5 155,185 - -0.9 155,185 - +1.6 155,185 - -0.6 155,185 - +2.1 155,208 + +1.0 155,209 - -0.1 155,266 + -0.1 155,267 - +0.6 155,267 - +1.8 155,277 + -0.2 155,277 + -0.1 155,277 + -0.7 155,277 + -0.2 155,277 + +0.5 155,278 - +1.3 155,278 - -0.3 155,278 - +1.0 155,278 - +0.6 155,278 - +2.3 155,278 - +2.1 155,368 - -0.9 155,438 + Psest_0157 0.15 -0.6 155,439 - Psest_0157 0.15 +0.2 155,540 + Psest_0157 0.28 -2.4 155,540 + Psest_0157 0.28 -1.8 155,576 + Psest_0157 0.33 -0.4 155,576 + Psest_0157 0.33 +1.6 155,576 + Psest_0157 0.33 +2.5 155,577 - Psest_0157 0.33 +0.9 155,603 + Psest_0157 0.37 +1.4 155,603 + Psest_0157 0.37 -0.6 155,603 + Psest_0157 0.37 -1.1 155,603 + Psest_0157 0.37 -1.0 155,604 - Psest_0157 0.37 +2.1 155,604 - Psest_0157 0.37 +2.8 155,744 + Psest_0157 0.56 +1.1 155,744 + Psest_0157 0.56 -0.8 155,744 + Psest_0157 0.56 -0.1 155,744 + Psest_0157 0.56 +1.2 155,744 + Psest_0157 0.56 +0.3 155,744 + Psest_0157 0.56 -0.6 155,745 - Psest_0157 0.56 -0.1 155,745 - Psest_0157 0.56 +0.7 155,745 - Psest_0157 0.56 +2.3 155,759 + Psest_0157 0.58 -1.5 155,759 + Psest_0157 0.58 +2.7 155,760 - Psest_0157 0.58 +1.5 155,760 - Psest_0157 0.58 -0.5 155,760 - Psest_0157 0.58 +0.5 155,760 - Psest_0157 0.58 +0.2 155,951 - Psest_0157 0.83 +1.4 155,963 + Psest_0157 0.85 -1.6 155,964 - Psest_0157 0.85 +1.1 156,069 - -0.9 156,069 - -1.0 156,323 + +1.5 156,344 + +1.7 156,345 - -0.9 156,345 - +0.3 156,345 - +0.3 156,442 + Psest_0158 0.18 +1.0 156,443 - Psest_0158 0.18 -1.6 156,443 - Psest_0158 0.18 +0.9 156,443 - Psest_0158 0.18 +1.2 156,443 - Psest_0158 0.18 +0.6 156,553 + Psest_0158 0.33 -0.2 156,554 - Psest_0158 0.33 -2.7 156,554 - Psest_0158 0.33 -0.5 156,554 - Psest_0158 0.33 +0.6 156,652 - Psest_0158 0.46 -0.5 156,736 + Psest_0158 0.57 -2.2 156,736 + Psest_0158 0.57 +2.0 156,874 + Psest_0158 0.76 -0.2 156,987 - +0.8 157,059 - -1.1 157,065 - +0.0
Or see this region's nucleotide sequence