Experiment: Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0104 and Psest_0105 overlap by 8 nucleotides Psest_0105 and Psest_0106 overlap by 4 nucleotides Psest_0106 and Psest_0107 are separated by 72 nucleotides Psest_0107 and Psest_0108 overlap by 4 nucleotides
Psest_0104: Psest_0104 - malonate decarboxylase acyl carrier protein, at 103,898 to 104,197
_0104
Psest_0105: Psest_0105 - malonate decarboxylase, beta subunit, at 104,190 to 105,056
_0105
Psest_0106: Psest_0106 - malonate decarboxylase, gamma subunit, at 105,053 to 105,838
_0106
Psest_0107: Psest_0107 - malonate decarboxylase holo-[acyl-carrier-protein] synthase, at 105,911 to 106,528
_0107
Psest_0108: Psest_0108 - malonate decarboxylase, epsilon subunit, at 106,525 to 107,451
_0108
Position (kb)
105
106 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 104.640 kb on + strand, within Psest_0105 at 104.640 kb on + strand, within Psest_0105 at 104.718 kb on + strand, within Psest_0105 at 104.719 kb on - strand, within Psest_0105 at 104.719 kb on - strand, within Psest_0105 at 104.797 kb on - strand, within Psest_0105 at 104.874 kb on + strand, within Psest_0105 at 104.874 kb on + strand, within Psest_0105 at 104.874 kb on + strand, within Psest_0105 at 104.874 kb on + strand, within Psest_0105 at 104.874 kb on + strand, within Psest_0105 at 104.874 kb on + strand, within Psest_0105 at 104.874 kb on + strand, within Psest_0105 at 104.874 kb on + strand, within Psest_0105 at 104.875 kb on - strand, within Psest_0105 at 104.875 kb on - strand, within Psest_0105 at 104.875 kb on - strand, within Psest_0105 at 105.155 kb on - strand, within Psest_0106 at 105.164 kb on + strand, within Psest_0106 at 105.164 kb on + strand, within Psest_0106 at 105.164 kb on + strand, within Psest_0106 at 105.165 kb on - strand, within Psest_0106 at 105.165 kb on - strand, within Psest_0106 at 105.325 kb on - strand, within Psest_0106 at 105.339 kb on - strand, within Psest_0106 at 105.621 kb on - strand, within Psest_0106 at 105.635 kb on + strand, within Psest_0106 at 105.635 kb on + strand, within Psest_0106 at 105.636 kb on - strand, within Psest_0106 at 105.636 kb on - strand, within Psest_0106 at 105.636 kb on - strand, within Psest_0106 at 105.636 kb on - strand, within Psest_0106 at 105.636 kb on - strand, within Psest_0106 at 105.908 kb on + strand at 105.908 kb on + strand at 105.908 kb on + strand at 105.909 kb on - strand at 105.909 kb on - strand at 105.909 kb on - strand at 105.909 kb on - strand at 106.230 kb on - strand, within Psest_0107 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.765 kb on + strand, within Psest_0108 at 106.766 kb on - strand, within Psest_0108 at 106.766 kb on - strand, within Psest_0108 at 106.766 kb on - strand, within Psest_0108 at 106.766 kb on - strand, within Psest_0108 at 106.766 kb on - strand, within Psest_0108 at 106.792 kb on + strand, within Psest_0108 at 106.792 kb on + strand, within Psest_0108 at 106.792 kb on + strand, within Psest_0108 at 106.826 kb on - strand, within Psest_0108 at 106.826 kb on - strand, within Psest_0108 at 106.826 kb on - strand, within Psest_0108
Per-strain Table
Position Strand Gene LocusTag Fraction Carbenicillin 0.008 mg/ml remove 104,640 + Psest_0105 0.52 -1.1 104,640 + Psest_0105 0.52 -1.9 104,718 + Psest_0105 0.61 +0.9 104,719 - Psest_0105 0.61 +1.3 104,719 - Psest_0105 0.61 -0.7 104,797 - Psest_0105 0.70 -1.6 104,874 + Psest_0105 0.79 +1.9 104,874 + Psest_0105 0.79 -1.7 104,874 + Psest_0105 0.79 +0.4 104,874 + Psest_0105 0.79 +1.1 104,874 + Psest_0105 0.79 -0.5 104,874 + Psest_0105 0.79 -0.6 104,874 + Psest_0105 0.79 +1.1 104,874 + Psest_0105 0.79 -1.6 104,875 - Psest_0105 0.79 -0.3 104,875 - Psest_0105 0.79 +1.3 104,875 - Psest_0105 0.79 +1.1 105,155 - Psest_0106 0.13 -0.9 105,164 + Psest_0106 0.14 -3.8 105,164 + Psest_0106 0.14 -1.3 105,164 + Psest_0106 0.14 -1.3 105,165 - Psest_0106 0.14 -0.8 105,165 - Psest_0106 0.14 -2.1 105,325 - Psest_0106 0.35 +0.4 105,339 - Psest_0106 0.36 -1.3 105,621 - Psest_0106 0.72 -1.2 105,635 + Psest_0106 0.74 -0.5 105,635 + Psest_0106 0.74 -0.8 105,636 - Psest_0106 0.74 +1.4 105,636 - Psest_0106 0.74 +0.9 105,636 - Psest_0106 0.74 -1.1 105,636 - Psest_0106 0.74 -0.4 105,636 - Psest_0106 0.74 -1.0 105,908 + +0.8 105,908 + -0.3 105,908 + -1.0 105,909 - -2.3 105,909 - -0.9 105,909 - +1.5 105,909 - -0.4 106,230 - Psest_0107 0.52 -1.4 106,765 + Psest_0108 0.26 +0.3 106,765 + Psest_0108 0.26 +1.7 106,765 + Psest_0108 0.26 +1.0 106,765 + Psest_0108 0.26 +0.4 106,765 + Psest_0108 0.26 +0.4 106,765 + Psest_0108 0.26 +0.3 106,765 + Psest_0108 0.26 +1.2 106,765 + Psest_0108 0.26 +0.6 106,766 - Psest_0108 0.26 +0.6 106,766 - Psest_0108 0.26 -0.9 106,766 - Psest_0108 0.26 +0.5 106,766 - Psest_0108 0.26 -1.2 106,766 - Psest_0108 0.26 -0.6 106,792 + Psest_0108 0.29 +0.5 106,792 + Psest_0108 0.29 +1.6 106,792 + Psest_0108 0.29 -0.1 106,826 - Psest_0108 0.32 +1.4 106,826 - Psest_0108 0.32 -2.3 106,826 - Psest_0108 0.32 -2.6
Or see this region's nucleotide sequence