Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0725

Experiment: Carbenicillin 0.0065 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0723 and Psest_0724 are separated by 156 nucleotidesPsest_0724 and Psest_0725 are separated by 104 nucleotidesPsest_0725 and Psest_0726 are separated by 16 nucleotides Psest_0723: Psest_0723 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 792,717 to 793,706 _0723 Psest_0724: Psest_0724 - Deoxyinosine 3'endonuclease (endonuclease V), at 793,863 to 794,591 _0724 Psest_0725: Psest_0725 - Negative regulator of beta-lactamase expression, at 794,696 to 795,247 _0725 Psest_0726: Psest_0726 - Membrane protein required for beta-lactamase induction, at 795,264 to 796,100 _0726 Position (kb) 794 795 796Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 793.714 kb on + strandat 793.718 kb on + strandat 793.718 kb on + strandat 793.718 kb on + strandat 793.718 kb on + strandat 793.718 kb on + strandat 793.718 kb on + strandat 793.718 kb on + strandat 793.718 kb on + strandat 793.718 kb on + strandat 793.718 kb on + strandat 793.719 kb on - strandat 793.719 kb on - strandat 793.719 kb on - strandat 793.719 kb on - strandat 793.719 kb on - strandat 793.719 kb on - strandat 793.719 kb on - strandat 793.719 kb on - strandat 794.008 kb on - strand, within Psest_0724at 794.121 kb on + strand, within Psest_0724at 794.121 kb on + strand, within Psest_0724at 794.121 kb on + strand, within Psest_0724at 794.121 kb on + strand, within Psest_0724at 794.122 kb on - strand, within Psest_0724at 794.122 kb on - strand, within Psest_0724at 794.266 kb on + strand, within Psest_0724at 794.395 kb on + strand, within Psest_0724at 794.605 kb on + strandat 794.781 kb on + strand, within Psest_0725at 794.781 kb on + strand, within Psest_0725at 794.793 kb on + strand, within Psest_0725at 794.794 kb on - strand, within Psest_0725at 794.794 kb on - strand, within Psest_0725at 795.055 kb on + strand, within Psest_0725at 795.238 kb on + strandat 795.239 kb on - strandat 795.239 kb on - strandat 795.415 kb on + strand, within Psest_0726at 795.519 kb on + strand, within Psest_0726at 795.519 kb on + strand, within Psest_0726at 795.520 kb on - strand, within Psest_0726at 795.520 kb on - strand, within Psest_0726at 795.690 kb on + strand, within Psest_0726at 795.690 kb on + strand, within Psest_0726at 795.690 kb on + strand, within Psest_0726at 795.690 kb on + strand, within Psest_0726at 795.690 kb on + strand, within Psest_0726at 795.690 kb on + strand, within Psest_0726at 795.690 kb on + strand, within Psest_0726at 795.690 kb on + strand, within Psest_0726at 795.690 kb on + strand, within Psest_0726at 795.690 kb on + strand, within Psest_0726at 795.690 kb on + strand, within Psest_0726at 795.691 kb on - strand, within Psest_0726at 795.691 kb on - strand, within Psest_0726at 795.691 kb on - strand, within Psest_0726at 795.691 kb on - strand, within Psest_0726at 795.691 kb on - strand, within Psest_0726at 795.691 kb on - strand, within Psest_0726at 795.691 kb on - strand, within Psest_0726at 795.854 kb on - strand, within Psest_0726at 795.859 kb on + strand, within Psest_0726at 795.934 kb on + strand, within Psest_0726at 795.934 kb on + strand, within Psest_0726at 795.934 kb on + strand, within Psest_0726at 795.934 kb on + strand, within Psest_0726at 795.934 kb on + strand, within Psest_0726at 795.935 kb on - strand, within Psest_0726at 795.935 kb on - strand, within Psest_0726at 796.045 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 0.0065 mg/ml
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793,714 + -0.2
793,718 + -1.1
793,718 + -0.2
793,718 + +0.4
793,718 + -1.6
793,718 + +0.9
793,718 + +0.5
793,718 + -0.3
793,718 + -0.4
793,718 + +0.8
793,718 + +0.3
793,719 - +3.9
793,719 - +1.1
793,719 - -0.3
793,719 - -1.9
793,719 - -0.1
793,719 - -0.5
793,719 - -2.1
793,719 - -0.4
794,008 - Psest_0724 0.20 +1.3
794,121 + Psest_0724 0.35 +0.7
794,121 + Psest_0724 0.35 +0.2
794,121 + Psest_0724 0.35 +3.9
794,121 + Psest_0724 0.35 -0.5
794,122 - Psest_0724 0.36 -0.1
794,122 - Psest_0724 0.36 -0.7
794,266 + Psest_0724 0.55 -1.4
794,395 + Psest_0724 0.73 +0.3
794,605 + +1.5
794,781 + Psest_0725 0.15 +0.3
794,781 + Psest_0725 0.15 -1.5
794,793 + Psest_0725 0.18 -1.2
794,794 - Psest_0725 0.18 -0.9
794,794 - Psest_0725 0.18 -0.7
795,055 + Psest_0725 0.65 -0.7
795,238 + +2.5
795,239 - -0.9
795,239 - +1.0
795,415 + Psest_0726 0.18 -1.5
795,519 + Psest_0726 0.30 -0.7
795,519 + Psest_0726 0.30 +0.0
795,520 - Psest_0726 0.31 -0.6
795,520 - Psest_0726 0.31 +0.2
795,690 + Psest_0726 0.51 +0.3
795,690 + Psest_0726 0.51 -0.4
795,690 + Psest_0726 0.51 -1.5
795,690 + Psest_0726 0.51 +0.6
795,690 + Psest_0726 0.51 +0.1
795,690 + Psest_0726 0.51 +0.8
795,690 + Psest_0726 0.51 -0.4
795,690 + Psest_0726 0.51 -1.0
795,690 + Psest_0726 0.51 -0.0
795,690 + Psest_0726 0.51 -0.0
795,690 + Psest_0726 0.51 -1.4
795,691 - Psest_0726 0.51 -0.4
795,691 - Psest_0726 0.51 +0.7
795,691 - Psest_0726 0.51 +1.5
795,691 - Psest_0726 0.51 -0.1
795,691 - Psest_0726 0.51 -0.3
795,691 - Psest_0726 0.51 -1.2
795,691 - Psest_0726 0.51 -1.0
795,854 - Psest_0726 0.70 -1.0
795,859 + Psest_0726 0.71 -0.6
795,934 + Psest_0726 0.80 -0.7
795,934 + Psest_0726 0.80 +1.1
795,934 + Psest_0726 0.80 -0.4
795,934 + Psest_0726 0.80 -2.1
795,934 + Psest_0726 0.80 -2.3
795,935 - Psest_0726 0.80 -3.1
795,935 - Psest_0726 0.80 +1.5
796,045 + -1.0

Or see this region's nucleotide sequence