Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2320

Experiment: Carbenicillin 0.0065 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2319 and Psest_2320 are separated by 74 nucleotidesPsest_2320 and Psest_2321 are separated by 92 nucleotidesPsest_2321 and Psest_2322 overlap by 4 nucleotides Psest_2319: Psest_2319 - aconitate hydratase 1/2-methylisocitrate dehydratase, Fe/S-dependent, at 2,471,353 to 2,473,956 _2319 Psest_2320: Psest_2320 - 2-methylcitrate synthase/citrate synthase II, at 2,474,031 to 2,475,158 _2320 Psest_2321: Psest_2321 - methylisocitrate lyase, at 2,475,251 to 2,476,144 _2321 Psest_2322: Psest_2322 - Transcriptional regulators, at 2,476,141 to 2,476,866 _2322 Position (kb) 2474 2475 2476Strain fitness (log2 ratio) -2 -1 0 1at 2474.078 kb on - strandat 2474.078 kb on - strandat 2474.078 kb on - strandat 2474.078 kb on - strandat 2474.218 kb on + strand, within Psest_2320at 2474.288 kb on - strand, within Psest_2320at 2474.368 kb on + strand, within Psest_2320at 2474.368 kb on + strand, within Psest_2320at 2474.539 kb on + strand, within Psest_2320at 2474.753 kb on - strand, within Psest_2320at 2474.753 kb on - strand, within Psest_2320at 2474.753 kb on - strand, within Psest_2320at 2474.891 kb on + strand, within Psest_2320at 2474.953 kb on + strand, within Psest_2320at 2474.953 kb on + strand, within Psest_2320at 2474.989 kb on + strand, within Psest_2320at 2474.990 kb on - strand, within Psest_2320at 2475.163 kb on - strandat 2475.253 kb on - strandat 2475.253 kb on - strandat 2475.309 kb on + strandat 2475.309 kb on + strandat 2475.309 kb on + strandat 2475.309 kb on + strandat 2475.309 kb on + strandat 2475.309 kb on + strandat 2475.309 kb on + strandat 2475.310 kb on - strandat 2475.310 kb on - strandat 2475.394 kb on - strand, within Psest_2321at 2475.486 kb on + strand, within Psest_2321at 2475.487 kb on - strand, within Psest_2321at 2475.487 kb on - strand, within Psest_2321at 2475.558 kb on + strand, within Psest_2321at 2475.558 kb on + strand, within Psest_2321at 2475.925 kb on + strand, within Psest_2321at 2475.932 kb on + strand, within Psest_2321at 2475.932 kb on + strand, within Psest_2321at 2475.932 kb on + strand, within Psest_2321at 2475.932 kb on + strand, within Psest_2321at 2475.933 kb on - strand, within Psest_2321at 2475.933 kb on - strand, within Psest_2321at 2475.933 kb on - strand, within Psest_2321at 2475.933 kb on - strand, within Psest_2321at 2475.933 kb on - strand, within Psest_2321at 2475.933 kb on - strand, within Psest_2321at 2476.017 kb on + strand, within Psest_2321at 2476.017 kb on + strand, within Psest_2321at 2476.017 kb on + strand, within Psest_2321at 2476.017 kb on + strand, within Psest_2321at 2476.017 kb on + strand, within Psest_2321at 2476.017 kb on + strand, within Psest_2321at 2476.018 kb on - strand, within Psest_2321at 2476.018 kb on - strand, within Psest_2321at 2476.018 kb on - strand, within Psest_2321at 2476.018 kb on - strand, within Psest_2321at 2476.018 kb on - strand, within Psest_2321

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbenicillin 0.0065 mg/ml
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2,474,078 - +0.5
2,474,078 - -2.6
2,474,078 - -0.6
2,474,078 - +0.1
2,474,218 + Psest_2320 0.17 -0.1
2,474,288 - Psest_2320 0.23 -2.1
2,474,368 + Psest_2320 0.30 -0.8
2,474,368 + Psest_2320 0.30 -1.0
2,474,539 + Psest_2320 0.45 -1.9
2,474,753 - Psest_2320 0.64 +0.2
2,474,753 - Psest_2320 0.64 -0.3
2,474,753 - Psest_2320 0.64 +0.4
2,474,891 + Psest_2320 0.76 -1.9
2,474,953 + Psest_2320 0.82 -0.2
2,474,953 + Psest_2320 0.82 +0.1
2,474,989 + Psest_2320 0.85 -0.7
2,474,990 - Psest_2320 0.85 +1.3
2,475,163 - -1.5
2,475,253 - -0.6
2,475,253 - +0.2
2,475,309 + +0.8
2,475,309 + +0.5
2,475,309 + +0.2
2,475,309 + -1.0
2,475,309 + -0.3
2,475,309 + -0.7
2,475,309 + +0.2
2,475,310 - -1.3
2,475,310 - -1.2
2,475,394 - Psest_2321 0.16 +1.6
2,475,486 + Psest_2321 0.26 +0.0
2,475,487 - Psest_2321 0.26 -0.4
2,475,487 - Psest_2321 0.26 -0.7
2,475,558 + Psest_2321 0.34 +0.1
2,475,558 + Psest_2321 0.34 +0.5
2,475,925 + Psest_2321 0.75 -1.4
2,475,932 + Psest_2321 0.76 +0.3
2,475,932 + Psest_2321 0.76 +0.9
2,475,932 + Psest_2321 0.76 +1.7
2,475,932 + Psest_2321 0.76 -0.1
2,475,933 - Psest_2321 0.76 -0.3
2,475,933 - Psest_2321 0.76 +1.1
2,475,933 - Psest_2321 0.76 -2.6
2,475,933 - Psest_2321 0.76 +1.3
2,475,933 - Psest_2321 0.76 -0.3
2,475,933 - Psest_2321 0.76 +1.1
2,476,017 + Psest_2321 0.86 -2.3
2,476,017 + Psest_2321 0.86 -0.6
2,476,017 + Psest_2321 0.86 -1.1
2,476,017 + Psest_2321 0.86 +1.0
2,476,017 + Psest_2321 0.86 +0.5
2,476,017 + Psest_2321 0.86 -2.5
2,476,018 - Psest_2321 0.86 +1.2
2,476,018 - Psest_2321 0.86 -0.3
2,476,018 - Psest_2321 0.86 +1.6
2,476,018 - Psest_2321 0.86 -2.5
2,476,018 - Psest_2321 0.86 -2.1

Or see this region's nucleotide sequence