Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25430 and BWI76_RS25435 are separated by 103 nucleotides BWI76_RS25435 and BWI76_RS25440 are separated by 50 nucleotides BWI76_RS25440 and BWI76_RS25445 are separated by 140 nucleotides BWI76_RS25445 and BWI76_RS25450 are separated by 5 nucleotides BWI76_RS25450 and BWI76_RS25455 are separated by 7 nucleotides
BWI76_RS25430: BWI76_RS25430 - membrane protein, at 5,285,409 to 5,285,672
_RS25430
BWI76_RS25435: BWI76_RS25435 - membrane protein, at 5,285,776 to 5,286,042
_RS25435
BWI76_RS25440: BWI76_RS25440 - putative barnase inhibitor, at 5,286,093 to 5,286,368
_RS25440
BWI76_RS25445: BWI76_RS25445 - p-hydroxybenzoic acid efflux pump subunit AaeB, at 5,286,509 to 5,288,476
_RS25445
BWI76_RS25450: BWI76_RS25450 - p-hydroxybenzoic acid efflux subunit AaeA, at 5,288,482 to 5,289,414
_RS25450
BWI76_RS25455: BWI76_RS25455 - protein AaeX, at 5,289,422 to 5,289,625
_RS25455
Position (kb) 5286
5287
5288
5289 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5285.545 kb on + strand, within BWI76_RS25430 at 5285.620 kb on + strand, within BWI76_RS25430 at 5285.632 kb on + strand, within BWI76_RS25430 at 5285.637 kb on + strand, within BWI76_RS25430 at 5285.717 kb on - strand at 5285.811 kb on + strand, within BWI76_RS25435 at 5285.891 kb on - strand, within BWI76_RS25435 at 5285.908 kb on + strand, within BWI76_RS25435 at 5286.018 kb on + strand at 5286.144 kb on - strand, within BWI76_RS25440 at 5286.144 kb on - strand, within BWI76_RS25440 at 5286.166 kb on - strand, within BWI76_RS25440 at 5286.241 kb on - strand, within BWI76_RS25440 at 5286.255 kb on - strand at 5286.268 kb on - strand, within BWI76_RS25440 at 5286.290 kb on + strand, within BWI76_RS25440 at 5286.298 kb on - strand, within BWI76_RS25440 at 5286.379 kb on - strand at 5286.420 kb on + strand at 5286.457 kb on + strand at 5286.591 kb on + strand at 5286.591 kb on + strand at 5286.953 kb on - strand, within BWI76_RS25445 at 5287.103 kb on - strand, within BWI76_RS25445 at 5287.387 kb on + strand, within BWI76_RS25445 at 5287.395 kb on - strand, within BWI76_RS25445 at 5287.579 kb on + strand, within BWI76_RS25445 at 5287.646 kb on - strand, within BWI76_RS25445 at 5287.693 kb on + strand, within BWI76_RS25445 at 5287.862 kb on - strand, within BWI76_RS25445 at 5287.921 kb on - strand, within BWI76_RS25445 at 5288.159 kb on + strand, within BWI76_RS25445 at 5288.298 kb on + strand at 5288.519 kb on - strand at 5288.576 kb on - strand, within BWI76_RS25450 at 5288.596 kb on - strand, within BWI76_RS25450 at 5288.607 kb on - strand, within BWI76_RS25450 at 5288.624 kb on + strand, within BWI76_RS25450 at 5288.734 kb on - strand, within BWI76_RS25450 at 5288.804 kb on + strand, within BWI76_RS25450 at 5288.828 kb on - strand, within BWI76_RS25450 at 5289.471 kb on + strand, within BWI76_RS25455 at 5289.471 kb on + strand, within BWI76_RS25455 at 5289.471 kb on + strand, within BWI76_RS25455
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM remove 5,285,545 + BWI76_RS25430 0.52 -0.4 5,285,620 + BWI76_RS25430 0.80 +0.5 5,285,632 + BWI76_RS25430 0.84 +0.3 5,285,637 + BWI76_RS25430 0.86 -0.0 5,285,717 - -0.5 5,285,811 + BWI76_RS25435 0.13 -0.3 5,285,891 - BWI76_RS25435 0.43 -0.4 5,285,908 + BWI76_RS25435 0.49 +1.1 5,286,018 + -0.2 5,286,144 - BWI76_RS25440 0.18 -0.1 5,286,144 - BWI76_RS25440 0.18 -0.1 5,286,166 - BWI76_RS25440 0.26 +0.7 5,286,241 - BWI76_RS25440 0.54 +0.7 5,286,255 - -0.6 5,286,268 - BWI76_RS25440 0.63 +0.4 5,286,290 + BWI76_RS25440 0.71 -1.9 5,286,298 - BWI76_RS25440 0.74 -0.3 5,286,379 - +0.5 5,286,420 + -2.8 5,286,457 + -0.2 5,286,591 + +0.1 5,286,591 + +1.2 5,286,953 - BWI76_RS25445 0.23 -0.9 5,287,103 - BWI76_RS25445 0.30 -0.4 5,287,387 + BWI76_RS25445 0.45 -0.7 5,287,395 - BWI76_RS25445 0.45 -0.6 5,287,579 + BWI76_RS25445 0.54 -1.3 5,287,646 - BWI76_RS25445 0.58 -2.8 5,287,693 + BWI76_RS25445 0.60 +0.2 5,287,862 - BWI76_RS25445 0.69 +0.9 5,287,921 - BWI76_RS25445 0.72 +1.1 5,288,159 + BWI76_RS25445 0.84 -0.5 5,288,298 + +0.4 5,288,519 - -0.9 5,288,576 - BWI76_RS25450 0.10 +0.2 5,288,596 - BWI76_RS25450 0.12 +0.2 5,288,607 - BWI76_RS25450 0.13 +0.2 5,288,624 + BWI76_RS25450 0.15 +0.7 5,288,734 - BWI76_RS25450 0.27 +0.3 5,288,804 + BWI76_RS25450 0.35 +0.3 5,288,828 - BWI76_RS25450 0.37 +2.1 5,289,471 + BWI76_RS25455 0.24 +0.1 5,289,471 + BWI76_RS25455 0.24 -1.2 5,289,471 + BWI76_RS25455 0.24 +0.0
Or see this region's nucleotide sequence