Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16160

Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16150 and BWI76_RS16155 overlap by 4 nucleotidesBWI76_RS16155 and BWI76_RS16160 are separated by 101 nucleotidesBWI76_RS16160 and BWI76_RS16165 are separated by 166 nucleotides BWI76_RS16150: BWI76_RS16150 - IclR family transcriptional regulator, at 3,350,484 to 3,351,290 _RS16150 BWI76_RS16155: BWI76_RS16155 - hypothetical protein, at 3,351,287 to 3,351,850 _RS16155 BWI76_RS16160: BWI76_RS16160 - LysR family transcriptional regulator, at 3,351,952 to 3,352,860 _RS16160 BWI76_RS16165: BWI76_RS16165 - peptidase M20, at 3,353,027 to 3,354,337 _RS16165 Position (kb) 3351 3352 3353Strain fitness (log2 ratio) -1 0 1 2at 3351.301 kb on - strandat 3351.925 kb on + strandat 3351.929 kb on - strandat 3351.978 kb on - strandat 3352.211 kb on + strand, within BWI76_RS16160at 3353.122 kb on + strandat 3353.211 kb on + strand, within BWI76_RS16165at 3353.271 kb on - strand, within BWI76_RS16165at 3353.544 kb on - strand, within BWI76_RS16165at 3353.690 kb on + strand, within BWI76_RS16165at 3353.833 kb on - strand, within BWI76_RS16165

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM
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3,351,301 - +1.3
3,351,925 + -0.2
3,351,929 - -0.0
3,351,978 - +0.3
3,352,211 + BWI76_RS16160 0.28 -0.9
3,353,122 + +2.6
3,353,211 + BWI76_RS16165 0.14 +0.0
3,353,271 - BWI76_RS16165 0.19 -0.3
3,353,544 - BWI76_RS16165 0.39 +0.4
3,353,690 + BWI76_RS16165 0.51 +0.1
3,353,833 - BWI76_RS16165 0.61 -0.2

Or see this region's nucleotide sequence