Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15130 and BWI76_RS15135 are separated by 300 nucleotides BWI76_RS15135 and BWI76_RS15140 are separated by 35 nucleotides BWI76_RS15140 and BWI76_RS15145 overlap by 4 nucleotides
BWI76_RS15130: BWI76_RS15130 - hypothetical protein, at 3,171,192 to 3,171,638
_RS15130
BWI76_RS15135: BWI76_RS15135 - hypothetical protein, at 3,171,939 to 3,172,229
_RS15135
BWI76_RS15140: BWI76_RS15140 - CusA/CzcA family heavy metal efflux RND transporter, at 3,172,265 to 3,175,378
_RS15140
BWI76_RS15145: BWI76_RS15145 - efflux transporter periplasmic adaptor subunit, at 3,175,375 to 3,176,844
_RS15145
Position (kb)
3172
3173
3174
3175
3176 Strain fitness (log2 ratio)
-1
0
1
2 at 3171.359 kb on + strand, within BWI76_RS15130 at 3171.413 kb on - strand, within BWI76_RS15130 at 3171.745 kb on - strand at 3171.904 kb on - strand at 3172.060 kb on - strand, within BWI76_RS15135 at 3172.090 kb on - strand, within BWI76_RS15135 at 3172.159 kb on + strand, within BWI76_RS15135 at 3172.167 kb on - strand, within BWI76_RS15135 at 3172.346 kb on + strand at 3172.542 kb on - strand at 3172.695 kb on + strand, within BWI76_RS15140 at 3172.703 kb on - strand, within BWI76_RS15140 at 3172.801 kb on + strand, within BWI76_RS15140 at 3173.250 kb on - strand, within BWI76_RS15140 at 3173.250 kb on - strand, within BWI76_RS15140 at 3173.368 kb on + strand, within BWI76_RS15140 at 3173.578 kb on - strand, within BWI76_RS15140 at 3173.597 kb on - strand, within BWI76_RS15140 at 3173.904 kb on - strand, within BWI76_RS15140 at 3173.910 kb on - strand, within BWI76_RS15140 at 3173.945 kb on + strand, within BWI76_RS15140 at 3173.960 kb on - strand, within BWI76_RS15140 at 3174.009 kb on - strand, within BWI76_RS15140 at 3174.366 kb on - strand, within BWI76_RS15140 at 3174.394 kb on + strand, within BWI76_RS15140 at 3174.404 kb on + strand, within BWI76_RS15140 at 3174.404 kb on + strand, within BWI76_RS15140 at 3174.404 kb on + strand, within BWI76_RS15140 at 3174.412 kb on - strand, within BWI76_RS15140 at 3174.422 kb on + strand, within BWI76_RS15140 at 3174.490 kb on + strand, within BWI76_RS15140 at 3174.769 kb on + strand, within BWI76_RS15140 at 3174.858 kb on - strand, within BWI76_RS15140 at 3175.080 kb on - strand at 3175.182 kb on - strand at 3175.386 kb on + strand at 3175.394 kb on - strand at 3175.394 kb on - strand at 3175.594 kb on - strand, within BWI76_RS15145 at 3175.681 kb on - strand, within BWI76_RS15145 at 3175.681 kb on - strand, within BWI76_RS15145 at 3175.683 kb on + strand, within BWI76_RS15145 at 3175.691 kb on - strand, within BWI76_RS15145 at 3176.300 kb on + strand, within BWI76_RS15145 at 3176.374 kb on - strand, within BWI76_RS15145
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM remove 3,171,359 + BWI76_RS15130 0.37 +0.5 3,171,413 - BWI76_RS15130 0.49 +0.3 3,171,745 - +1.0 3,171,904 - +0.2 3,172,060 - BWI76_RS15135 0.42 +0.6 3,172,090 - BWI76_RS15135 0.52 +0.9 3,172,159 + BWI76_RS15135 0.76 +0.0 3,172,167 - BWI76_RS15135 0.78 +0.2 3,172,346 + +1.8 3,172,542 - +0.2 3,172,695 + BWI76_RS15140 0.14 +0.5 3,172,703 - BWI76_RS15140 0.14 +0.2 3,172,801 + BWI76_RS15140 0.17 +1.2 3,173,250 - BWI76_RS15140 0.32 +0.1 3,173,250 - BWI76_RS15140 0.32 +0.2 3,173,368 + BWI76_RS15140 0.35 +0.6 3,173,578 - BWI76_RS15140 0.42 +0.5 3,173,597 - BWI76_RS15140 0.43 +0.3 3,173,904 - BWI76_RS15140 0.53 +0.6 3,173,910 - BWI76_RS15140 0.53 +1.5 3,173,945 + BWI76_RS15140 0.54 +0.3 3,173,960 - BWI76_RS15140 0.54 -0.2 3,174,009 - BWI76_RS15140 0.56 +1.1 3,174,366 - BWI76_RS15140 0.67 +0.3 3,174,394 + BWI76_RS15140 0.68 -0.9 3,174,404 + BWI76_RS15140 0.69 +0.1 3,174,404 + BWI76_RS15140 0.69 +1.0 3,174,404 + BWI76_RS15140 0.69 +1.3 3,174,412 - BWI76_RS15140 0.69 -0.1 3,174,422 + BWI76_RS15140 0.69 -0.8 3,174,490 + BWI76_RS15140 0.71 -0.4 3,174,769 + BWI76_RS15140 0.80 -0.5 3,174,858 - BWI76_RS15140 0.83 +0.6 3,175,080 - +0.1 3,175,182 - +0.3 3,175,386 + +0.3 3,175,394 - -0.0 3,175,394 - +0.3 3,175,594 - BWI76_RS15145 0.15 -0.1 3,175,681 - BWI76_RS15145 0.21 -0.0 3,175,681 - BWI76_RS15145 0.21 +0.3 3,175,683 + BWI76_RS15145 0.21 -0.2 3,175,691 - BWI76_RS15145 0.21 +0.3 3,176,300 + BWI76_RS15145 0.63 +0.2 3,176,374 - BWI76_RS15145 0.68 -0.6
Or see this region's nucleotide sequence