Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10785 and BWI76_RS10790 overlap by 4 nucleotides BWI76_RS10790 and BWI76_RS10795 are separated by 193 nucleotides BWI76_RS10795 and BWI76_RS10800 are separated by 427 nucleotides
BWI76_RS10785: BWI76_RS10785 - transcriptional regulator, at 2,276,216 to 2,276,854
_RS10785
BWI76_RS10790: BWI76_RS10790 - hypothetical protein, at 2,276,851 to 2,277,246
_RS10790
BWI76_RS10795: BWI76_RS10795 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 2,277,440 to 2,281,402
_RS10795
BWI76_RS10800: BWI76_RS10800 - sodium:proline symporter, at 2,281,830 to 2,283,338
_RS10800
Position (kb)
2277
2278
2279
2280
2281
2282 Strain fitness (log2 ratio)
-1
0
1
2 at 2276.482 kb on - strand, within BWI76_RS10785 at 2276.525 kb on - strand, within BWI76_RS10785 at 2276.525 kb on - strand, within BWI76_RS10785 at 2276.728 kb on + strand, within BWI76_RS10785 at 2276.728 kb on + strand, within BWI76_RS10785 at 2276.942 kb on + strand, within BWI76_RS10790 at 2277.019 kb on + strand, within BWI76_RS10790 at 2277.082 kb on + strand, within BWI76_RS10790 at 2277.125 kb on + strand, within BWI76_RS10790 at 2277.133 kb on - strand, within BWI76_RS10790 at 2277.303 kb on + strand at 2277.330 kb on - strand at 2277.515 kb on - strand at 2277.846 kb on - strand, within BWI76_RS10795 at 2277.961 kb on + strand, within BWI76_RS10795 at 2278.182 kb on + strand, within BWI76_RS10795 at 2278.635 kb on + strand, within BWI76_RS10795 at 2278.709 kb on + strand, within BWI76_RS10795 at 2279.086 kb on + strand, within BWI76_RS10795 at 2279.486 kb on + strand, within BWI76_RS10795 at 2279.608 kb on - strand, within BWI76_RS10795 at 2280.414 kb on - strand, within BWI76_RS10795 at 2280.519 kb on + strand, within BWI76_RS10795 at 2280.737 kb on + strand, within BWI76_RS10795 at 2280.848 kb on + strand, within BWI76_RS10795 at 2280.922 kb on + strand, within BWI76_RS10795 at 2280.930 kb on - strand, within BWI76_RS10795 at 2281.167 kb on + strand at 2281.183 kb on - strand at 2281.375 kb on + strand at 2281.383 kb on - strand at 2281.383 kb on - strand at 2281.456 kb on - strand at 2281.693 kb on + strand at 2281.736 kb on - strand at 2281.736 kb on - strand at 2281.765 kb on - strand at 2281.833 kb on + strand at 2281.915 kb on - strand at 2281.951 kb on + strand at 2281.951 kb on + strand at 2281.951 kb on + strand at 2281.951 kb on + strand at 2281.951 kb on + strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2281.959 kb on - strand at 2282.067 kb on - strand, within BWI76_RS10800 at 2282.070 kb on + strand, within BWI76_RS10800 at 2282.072 kb on - strand, within BWI76_RS10800 at 2282.073 kb on - strand, within BWI76_RS10800 at 2282.073 kb on - strand, within BWI76_RS10800 at 2282.085 kb on - strand, within BWI76_RS10800 at 2282.102 kb on + strand, within BWI76_RS10800 at 2282.102 kb on + strand, within BWI76_RS10800 at 2282.108 kb on + strand, within BWI76_RS10800 at 2282.195 kb on - strand, within BWI76_RS10800 at 2282.195 kb on - strand, within BWI76_RS10800 at 2282.270 kb on - strand, within BWI76_RS10800 at 2282.272 kb on + strand, within BWI76_RS10800 at 2282.280 kb on - strand, within BWI76_RS10800 at 2282.280 kb on - strand, within BWI76_RS10800 at 2282.282 kb on + strand, within BWI76_RS10800 at 2282.282 kb on + strand, within BWI76_RS10800 at 2282.285 kb on - strand, within BWI76_RS10800 at 2282.301 kb on - strand, within BWI76_RS10800 at 2282.349 kb on - strand, within BWI76_RS10800 at 2282.386 kb on - strand, within BWI76_RS10800 at 2282.394 kb on + strand, within BWI76_RS10800 at 2282.402 kb on - strand, within BWI76_RS10800
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM remove 2,276,482 - BWI76_RS10785 0.42 -0.4 2,276,525 - BWI76_RS10785 0.48 -0.7 2,276,525 - BWI76_RS10785 0.48 -0.6 2,276,728 + BWI76_RS10785 0.80 +0.2 2,276,728 + BWI76_RS10785 0.80 +0.0 2,276,942 + BWI76_RS10790 0.23 +0.4 2,277,019 + BWI76_RS10790 0.42 +1.0 2,277,082 + BWI76_RS10790 0.58 +0.6 2,277,125 + BWI76_RS10790 0.69 -0.3 2,277,133 - BWI76_RS10790 0.71 -0.2 2,277,303 + +0.3 2,277,330 - -0.4 2,277,515 - -1.4 2,277,846 - BWI76_RS10795 0.10 +0.5 2,277,961 + BWI76_RS10795 0.13 +0.1 2,278,182 + BWI76_RS10795 0.19 +0.1 2,278,635 + BWI76_RS10795 0.30 +0.4 2,278,709 + BWI76_RS10795 0.32 -1.2 2,279,086 + BWI76_RS10795 0.42 +0.7 2,279,486 + BWI76_RS10795 0.52 -0.0 2,279,608 - BWI76_RS10795 0.55 +0.2 2,280,414 - BWI76_RS10795 0.75 +0.1 2,280,519 + BWI76_RS10795 0.78 +0.2 2,280,737 + BWI76_RS10795 0.83 -0.5 2,280,848 + BWI76_RS10795 0.86 -0.6 2,280,922 + BWI76_RS10795 0.88 -0.2 2,280,930 - BWI76_RS10795 0.88 +0.9 2,281,167 + -0.4 2,281,183 - -0.7 2,281,375 + +1.8 2,281,383 - -0.7 2,281,383 - +0.1 2,281,456 - +0.3 2,281,693 + -0.8 2,281,736 - -0.3 2,281,736 - +0.4 2,281,765 - -0.2 2,281,833 + -0.1 2,281,915 - -0.5 2,281,951 + -0.0 2,281,951 + -0.2 2,281,951 + +0.3 2,281,951 + -0.4 2,281,951 + +1.3 2,281,959 - -0.4 2,281,959 - -0.3 2,281,959 - +0.7 2,281,959 - -1.3 2,281,959 - +0.7 2,281,959 - +0.6 2,281,959 - -0.0 2,281,959 - -1.0 2,281,959 - +0.3 2,282,067 - BWI76_RS10800 0.16 +0.7 2,282,070 + BWI76_RS10800 0.16 +0.4 2,282,072 - BWI76_RS10800 0.16 -0.9 2,282,073 - BWI76_RS10800 0.16 +0.0 2,282,073 - BWI76_RS10800 0.16 +0.3 2,282,085 - BWI76_RS10800 0.17 -0.5 2,282,102 + BWI76_RS10800 0.18 +0.2 2,282,102 + BWI76_RS10800 0.18 +0.9 2,282,108 + BWI76_RS10800 0.18 +0.1 2,282,195 - BWI76_RS10800 0.24 -0.9 2,282,195 - BWI76_RS10800 0.24 +0.2 2,282,270 - BWI76_RS10800 0.29 -1.6 2,282,272 + BWI76_RS10800 0.29 -0.1 2,282,280 - BWI76_RS10800 0.30 -0.6 2,282,280 - BWI76_RS10800 0.30 -0.5 2,282,282 + BWI76_RS10800 0.30 +1.8 2,282,282 + BWI76_RS10800 0.30 -0.0 2,282,285 - BWI76_RS10800 0.30 -0.8 2,282,301 - BWI76_RS10800 0.31 -0.4 2,282,349 - BWI76_RS10800 0.34 -0.4 2,282,386 - BWI76_RS10800 0.37 -0.2 2,282,394 + BWI76_RS10800 0.37 -0.6 2,282,402 - BWI76_RS10800 0.38 -0.6
Or see this region's nucleotide sequence