Experiment: RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10300 and BWI76_RS10305 are separated by 167 nucleotides BWI76_RS10305 and BWI76_RS10310 are separated by 100 nucleotides BWI76_RS10310 and BWI76_RS10315 are separated by 201 nucleotides
BWI76_RS10300: BWI76_RS10300 - MFS transporter, at 2,180,727 to 2,182,163
_RS10300
BWI76_RS10305: BWI76_RS10305 - transposase, at 2,182,331 to 2,182,927
_RS10305
BWI76_RS10310: BWI76_RS10310 - DNA-binding transcriptional regulator RamA, at 2,183,028 to 2,183,372
_RS10310
BWI76_RS10315: BWI76_RS10315 - MFS transporter, at 2,183,574 to 2,184,866
_RS10315
Position (kb)
2183
2184 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2182.246 kb on - strand at 2182.410 kb on + strand, within BWI76_RS10305 at 2182.495 kb on + strand, within BWI76_RS10305 at 2182.622 kb on + strand, within BWI76_RS10305 at 2182.622 kb on + strand, within BWI76_RS10305 at 2182.640 kb on + strand, within BWI76_RS10305 at 2182.651 kb on + strand, within BWI76_RS10305 at 2182.681 kb on + strand, within BWI76_RS10305 at 2182.681 kb on + strand, within BWI76_RS10305 at 2182.681 kb on + strand, within BWI76_RS10305 at 2182.705 kb on + strand, within BWI76_RS10305 at 2182.705 kb on + strand, within BWI76_RS10305 at 2182.711 kb on + strand, within BWI76_RS10305 at 2182.751 kb on + strand, within BWI76_RS10305 at 2182.751 kb on + strand, within BWI76_RS10305 at 2182.772 kb on + strand, within BWI76_RS10305 at 2182.930 kb on - strand at 2182.930 kb on - strand at 2182.943 kb on + strand at 2183.093 kb on + strand, within BWI76_RS10310 at 2183.098 kb on + strand, within BWI76_RS10310 at 2183.098 kb on + strand, within BWI76_RS10310 at 2183.098 kb on + strand, within BWI76_RS10310 at 2183.166 kb on + strand, within BWI76_RS10310 at 2183.170 kb on - strand, within BWI76_RS10310 at 2183.183 kb on + strand, within BWI76_RS10310 at 2183.183 kb on - strand, within BWI76_RS10310 at 2183.262 kb on - strand, within BWI76_RS10310 at 2183.272 kb on + strand, within BWI76_RS10310 at 2183.272 kb on + strand, within BWI76_RS10310 at 2183.280 kb on - strand, within BWI76_RS10310 at 2183.280 kb on - strand, within BWI76_RS10310 at 2183.280 kb on - strand, within BWI76_RS10310 at 2183.291 kb on - strand, within BWI76_RS10310 at 2183.291 kb on - strand, within BWI76_RS10310 at 2183.384 kb on - strand at 2183.390 kb on + strand at 2183.397 kb on + strand at 2183.398 kb on - strand at 2183.405 kb on - strand at 2183.405 kb on - strand at 2183.405 kb on - strand at 2183.405 kb on - strand at 2183.406 kb on + strand at 2183.444 kb on + strand at 2183.452 kb on - strand at 2183.465 kb on + strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.473 kb on - strand at 2183.572 kb on + strand at 2183.580 kb on - strand at 2183.659 kb on + strand at 2183.702 kb on - strand at 2183.702 kb on - strand at 2183.720 kb on + strand, within BWI76_RS10315 at 2183.728 kb on - strand, within BWI76_RS10315 at 2183.759 kb on - strand, within BWI76_RS10315 at 2183.770 kb on - strand, within BWI76_RS10315 at 2183.777 kb on - strand, within BWI76_RS10315 at 2183.777 kb on - strand, within BWI76_RS10315 at 2183.777 kb on - strand, within BWI76_RS10315 at 2183.777 kb on - strand, within BWI76_RS10315 at 2183.782 kb on + strand, within BWI76_RS10315 at 2183.790 kb on - strand, within BWI76_RS10315 at 2183.839 kb on + strand, within BWI76_RS10315 at 2183.839 kb on + strand, within BWI76_RS10315 at 2183.847 kb on - strand at 2184.087 kb on + strand, within BWI76_RS10315 at 2184.097 kb on + strand, within BWI76_RS10315 at 2184.325 kb on + strand, within BWI76_RS10315 at 2184.325 kb on + strand, within BWI76_RS10315 at 2184.332 kb on - strand, within BWI76_RS10315
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 600 mM and Betaine 100 uM remove 2,182,246 - -1.6 2,182,410 + BWI76_RS10305 0.13 +0.2 2,182,495 + BWI76_RS10305 0.27 +2.8 2,182,622 + BWI76_RS10305 0.49 -0.6 2,182,622 + BWI76_RS10305 0.49 +0.1 2,182,640 + BWI76_RS10305 0.52 -1.4 2,182,651 + BWI76_RS10305 0.54 +0.0 2,182,681 + BWI76_RS10305 0.59 +0.5 2,182,681 + BWI76_RS10305 0.59 -0.1 2,182,681 + BWI76_RS10305 0.59 +0.5 2,182,705 + BWI76_RS10305 0.63 -0.6 2,182,705 + BWI76_RS10305 0.63 -0.2 2,182,711 + BWI76_RS10305 0.64 -0.4 2,182,751 + BWI76_RS10305 0.70 +0.7 2,182,751 + BWI76_RS10305 0.70 -0.5 2,182,772 + BWI76_RS10305 0.74 -1.2 2,182,930 - -0.7 2,182,930 - +1.3 2,182,943 + +0.1 2,183,093 + BWI76_RS10310 0.19 -1.6 2,183,098 + BWI76_RS10310 0.20 -0.3 2,183,098 + BWI76_RS10310 0.20 -0.7 2,183,098 + BWI76_RS10310 0.20 +1.3 2,183,166 + BWI76_RS10310 0.40 -2.2 2,183,170 - BWI76_RS10310 0.41 -0.3 2,183,183 + BWI76_RS10310 0.45 +0.3 2,183,183 - BWI76_RS10310 0.45 -0.5 2,183,262 - BWI76_RS10310 0.68 +2.3 2,183,272 + BWI76_RS10310 0.71 -0.2 2,183,272 + BWI76_RS10310 0.71 +1.0 2,183,280 - BWI76_RS10310 0.73 -0.7 2,183,280 - BWI76_RS10310 0.73 -0.3 2,183,280 - BWI76_RS10310 0.73 +1.3 2,183,291 - BWI76_RS10310 0.76 -1.6 2,183,291 - BWI76_RS10310 0.76 +1.3 2,183,384 - -0.6 2,183,390 + +0.6 2,183,397 + -1.3 2,183,398 - +0.3 2,183,405 - +0.7 2,183,405 - +0.6 2,183,405 - -2.1 2,183,405 - +0.5 2,183,406 + +0.3 2,183,444 + +0.7 2,183,452 - -0.2 2,183,465 + -1.2 2,183,473 - -0.6 2,183,473 - -1.2 2,183,473 - +0.3 2,183,473 - -0.0 2,183,473 - +0.2 2,183,473 - +1.0 2,183,473 - +0.2 2,183,572 + -1.1 2,183,580 - +0.1 2,183,659 + -1.3 2,183,702 - +1.1 2,183,702 - +0.4 2,183,720 + BWI76_RS10315 0.11 -0.8 2,183,728 - BWI76_RS10315 0.12 -1.1 2,183,759 - BWI76_RS10315 0.14 -0.5 2,183,770 - BWI76_RS10315 0.15 -1.1 2,183,777 - BWI76_RS10315 0.16 +0.5 2,183,777 - BWI76_RS10315 0.16 -1.0 2,183,777 - BWI76_RS10315 0.16 -0.5 2,183,777 - BWI76_RS10315 0.16 +0.6 2,183,782 + BWI76_RS10315 0.16 -0.5 2,183,790 - BWI76_RS10315 0.17 +0.9 2,183,839 + BWI76_RS10315 0.20 +0.0 2,183,839 + BWI76_RS10315 0.20 -1.1 2,183,847 - +1.2 2,184,087 + BWI76_RS10315 0.40 +0.8 2,184,097 + BWI76_RS10315 0.40 +0.0 2,184,325 + BWI76_RS10315 0.58 -0.5 2,184,325 + BWI76_RS10315 0.58 +1.0 2,184,332 - BWI76_RS10315 0.59 -0.0
Or see this region's nucleotide sequence